Evolutionary pathways of repeat protein topology in bacterial outer membrane proteins [PDF]
eLife, 2018 Outer membrane proteins (OMPs) are the proteins in the surface of Gram-negative bacteria. These proteins have diverse functions but a single topology: the β-barrel.
Meghan Whitney Franklin +5 more
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Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel [PDF]
Nature Communications, 2019 The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). Here authors develop a method to trap an E.
Matthew T. Doyle, Harris D. Bernstein
doaj +3 more sources
Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins.
Curr Opin Struct Biol, 2015 Highlights • We discuss spatiotemporal patterning in the bacterial outer membrane.• Promiscuous interactions between outer membrane proteins govern their behaviour.• Turnover and biogenesis of outer membrane proteins linked to formation of clusters ...
Kleanthous C, Rassam P, Baumann CG.
europepmc +4 more sources
Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins
mBio, 2017 The Gram-negative bacterial cell wall component lipopolysaccharide (LPS) is recognized by the noncanonical inflammasome protein caspase-11 in the cytosol of infected host cells and thereby prompts an inflammatory immune response linked to sepsis.
Ryan Finethy +8 more
doaj +4 more sources
Bacterial Outer Membrane Proteins Are Targeted to the Bam Complex by Two Parallel Mechanisms. [PDF]
mBio, 2021 Proteins that are embedded in the outer membrane of Gram-negative bacteria (OMPs) play an important role in protecting the cell from harmful chemicals. OMPs share a architecture and often contain a conserved sequence motif (β motif) of unknown function ...
Wang X, Peterson JH, Bernstein HD.
europepmc +2 more sources
How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins. [PDF]
Nat Commun, 2018 The spatiotemporal organisation of membranes is often characterised by the formation of large protein clusters. In Escherichia coli, outer membrane protein (OMP) clustering leads to OMP islands, the formation of which underpins OMP turnover and drives ...
Chavent M +10 more
europepmc +2 more sources
The Bam complex catalyzes efficient insertion of bacterial outer membrane proteins into membrane vesicles of variable lipid composition. [PDF]
J Biol Chem, 2018 Most proteins that reside in the bacterial outer membrane (OM) have a distinctive “β-barrel” architecture, but the assembly of these proteins is poorly understood.
Hussain S, Bernstein HD.
europepmc +2 more sources
The structure of bacterial outer membrane proteins.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2002 Integral membrane proteins come in two types, alpha-helical and beta-barrel proteins. In both types, all hydrogen bonding donors and acceptors of the polypeptide backbone are completely compensated and buried while nonpolar side chains point to the membrane. The alpha-helical type is more abundant and occurs in cytoplasmic (or inner) membranes, whereas
G. Schulz
semanticscholar +4 more sources
Interactions between folding factors and bacterial outer membrane proteins [PDF]
Molecular Microbiology, 2005 SummaryThe outer membrane is the first line of contact between Gram‐negative bacteria and their external environment. Embedded in the outer membrane are integral outer membrane proteins (OMPs) that perform a diverse range of tasks. OMPs are synthesized in the cytoplasm and are translocated across the inner membrane and probably diffuse through the ...
J. Mogensen, D. Otzen
semanticscholar +4 more sources
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane [PDF]
Philosophical Transactions of the Royal Society B: Biological Sciences, 2017 The bacterial outer membrane contains phospholipids in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet. Both proteins and LPS must be frequently inserted into the outer membrane to preserve its integrity. The protein complex that inserts LPS into the outer membrane is called LptDE, and consists of an integral membrane protein, LptD,
I. Botos, N. Noinaj, S. Buchanan
semanticscholar +3 more sources