Evolutionary pathways of repeat protein topology in bacterial outer membrane proteins [PDF]
eLife, 2018 Outer membrane proteins (OMPs) are the proteins in the surface of Gram-negative bacteria. These proteins have diverse functions but a single topology: the β-barrel.
Meghan Whitney Franklin +5 more
doaj +5 more sources
Entry and exit of bacterial outer membrane proteins. [PDF]
Trends Microbiol, 2015 The sites of new outer membrane protein (OMP) deposition and the fate of pre-existing OMPs are still enigmatic despite numerous concerted efforts. Rassam et al. identified mid-cell regions as the primary entry points for new OMP insertion in clusters, driving the pre-existing OMP clusters towards cell poles for long-term storage.
Misra R.
europepmc +6 more sources
Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli [PDF]
MicrobiologyOpen, 2022 Gaining structural information on membrane proteins in their native lipid environment is a long‐standing challenge in molecular biology. Instead, it is common to employ membrane mimetics, which has been shown to affect protein structure, dynamics, and ...
Mohammed Mouhib, Celestine N. Chi
doaj +2 more sources
Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity [PDF]
eLifeOuter membrane proteins (OMPs) are essential components of the outer membrane of Gram-negative bacteria. In terms of protein targeting and assembly, the current dogma holds that a ‘β-signal’ imprinted in the final β-strand of the OMP engages the β-barrel
Edward M Germany +15 more
doaj +2 more sources
Bio-Protocol, 2020 Bacterial outer membrane vesicles (OMVs) are naturally formed by budding from the outer membrane of Gram-negative bacteria. OMVs consist of a lipid bilayer identical in composition to the original outer membrane and contain periplasmic content within ...
Johannes Thoma, Björn Burmann
doaj +3 more sources
Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel [PDF]
Nature Communications, 2019 The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). Here authors develop a method to trap an E.
Matthew T. Doyle, Harris D. Bernstein
doaj +2 more sources
The density of Braun's Lipoprotein determines vesicle production in E. coli. [PDF]
PLoS ONEProduction of extracellular vesicles by Gram-negative bacteria is known to be associated with blebbing of the outer membrane. Several proteins within Gram-negative bacteria crosslink the outer membrane to the cell wall, and thereby stabilize the cell ...
Brian P Weaver +4 more
doaj +2 more sources
Bacterial Adaptor Membrane Fusion Proteins and the Structurally Dissimilar Outer Membrane Auxiliary Proteins Have Exchanged Central Domains in α-Proteobacteria [PDF]
International Journal of Microbiology, 2010 Transport systems frequently include auxiliary proteins that perform subfunctions within the transporter protein complex. Two such proteins found in Gram-negative bacteria are the Membrane Fusion Proteins (MFPs) and the Outer Membrane Auxiliary (OMA ...
Anthony Y. Xiao +2 more
doaj +2 more sources
The Bam complex catalyzes efficient insertion of bacterial outer membrane proteins into membrane vesicles of variable lipid composition. [PDF]
J Biol Chem, 2018 Hussain S, Bernstein HD.
europepmc +2 more sources
The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins in vitro. [PDF]
Nat CommunWang X, Nyenhuis SB, Bernstein HD.
europepmc +3 more sources