Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity [PDF]
eLifeOuter membrane proteins (OMPs) are essential components of the outer membrane of Gram-negative bacteria. In terms of protein targeting and assembly, the current dogma holds that a ‘β-signal’ imprinted in the final β-strand of the OMP engages the β-barrel
Edward M Germany +15 more
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Evolutionary pathways of repeat protein topology in bacterial outer membrane proteins [PDF]
eLife, 2018 Outer membrane proteins (OMPs) are the proteins in the surface of Gram-negative bacteria. These proteins have diverse functions but a single topology: the β-barrel.
Meghan Whitney Franklin +5 more
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Construction of a bacterial surface display system based on outer membrane protein F [PDF]
Microbial Cell Factories, 2019 Background Bacterial surface display systems were developed to surface expose heterologous proteins or peptides for different applications, such as peptide libraries screening and live bacterial vaccine design.
Tingting Chen +12 more
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Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins.
Curr Opin Struct Biol, 2015 It has until recently been unclear whether outer membrane proteins (OMPs) of Gram-negative bacteria are organized or distributed randomly. Studies now suggest promiscuous protein-protein interactions (PPIs) between β-barrel OMPs in Escherichia coli govern their local and global dynamics, engender spatiotemporal patterning of the outer membrane into ...
Kleanthous C, Rassam P, Baumann CG.
europepmc +4 more sources
Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli [PDF]
MicrobiologyOpen, 2022 Gaining structural information on membrane proteins in their native lipid environment is a long‐standing challenge in molecular biology. Instead, it is common to employ membrane mimetics, which has been shown to affect protein structure, dynamics, and ...
Mohammed Mouhib, Celestine N. Chi
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Bio-Protocol, 2020 Bacterial outer membrane vesicles (OMVs) are naturally formed by budding from the outer membrane of Gram-negative bacteria. OMVs consist of a lipid bilayer identical in composition to the original outer membrane and contain periplasmic content within ...
Johannes Thoma, Björn Burmann
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Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel [PDF]
Nature Communications, 2019 The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). Here authors develop a method to trap an E.
Matthew T. Doyle, Harris D. Bernstein
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Cryo-EM structure of the QseG-QseE complex reveals an accessory protein-driven two-component system activation mechanism [PDF]
mBioThe two-component system (TCS) enables bacteria to sense and respond to environmental changes through histidine kinase-mediated signaling cascades. Although the core components of TCSs have been extensively studied, the molecular basis of accessory ...
Piqian Gong +7 more
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The density of Braun's Lipoprotein determines vesicle production in E. coli. [PDF]
PLoS ONEProduction of extracellular vesicles by Gram-negative bacteria is known to be associated with blebbing of the outer membrane. Several proteins within Gram-negative bacteria crosslink the outer membrane to the cell wall, and thereby stabilize the cell ...
Brian P Weaver +4 more
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Gram-negative bacterial outer membrane proteins and lipopolysaccharides key factors linking chicken coop environment and oxidative stress. [PDF]
Redox BiolLiu X +5 more
europepmc +2 more sources