Results 1 to 10 of about 148,445 (252)

Evolutionary pathways of repeat protein topology in bacterial outer membrane proteins [PDF]

eLife, 2018
Outer membrane proteins (OMPs) are the proteins in the surface of Gram-negative bacteria. These proteins have diverse functions but a single topology: the β-barrel.
Meghan Whitney Franklin, Sergey Nepomnyachyi, Ryan Feehan, Nir Ben-Tal, Rachel Kolodny, Joanna SG Slusky   +5 more
doaj   +4 more sources

Insertion of proteins and lipopolysaccharide into the bacterial outer membrane [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2017
The bacterial outer membrane contains phospholipids in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet. Both proteins and LPS must be frequently inserted into the outer membrane to preserve its integrity. The protein complex that inserts LPS into the outer membrane is called LptDE, and consists of an integral membrane protein, LptD,
Nicholas Noinaj, Susan Buchanan, István Botos   +2 more
exaly   +3 more sources

Entry and exit of bacterial outer membrane proteins. [PDF]

Trends Microbiol, 2015
The sites of new outer membrane protein (OMP) deposition and the fate of pre-existing OMPs are still enigmatic despite numerous concerted efforts. Rassam et al. identified mid-cell regions as the primary entry points for new OMP insertion in clusters, driving the pre-existing OMP clusters towards cell poles for long-term storage.
Misra R.
europepmc   +3 more sources

Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli [PDF]

MicrobiologyOpen, 2022
Gaining structural information on membrane proteins in their native lipid environment is a long‐standing challenge in molecular biology. Instead, it is common to employ membrane mimetics, which has been shown to affect protein structure, dynamics, and ...
Mohammed Mouhib, Celestine N. Chi
doaj   +2 more sources

Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity [PDF]

eLife
Outer membrane proteins (OMPs) are essential components of the outer membrane of Gram-negative bacteria. In terms of protein targeting and assembly, the current dogma holds that a ‘β-signal’ imprinted in the final β-strand of the OMP engages the β-barrel
Edward M Germany, Nakajohn Thewasano, Kenichiro Imai, Yuki Maruno, Rebecca S Bamert, Christopher J Stubenrauch, Rhys A Dunstan, Yue Ding, Yukari Nakajima, XiangFeng Lai, Chaille T Webb, Kentaro Hidaka, Kher Shing Tan, Hsinhui Shen, Trevor Lithgow, Takuya Shiota   +15 more
doaj   +2 more sources

Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins

Bio-Protocol, 2021
Lipopolysaccharides (LPS) (or lipooligosaccharides [LOS], which lack the O-antigen side chains characteristic of LPS), and outer membrane proteins (OMP) are major cell-surface molecules in the outer membrane (OM) of gram-negative bacteria.
Yue Lee, Thomas Inzana
doaj   +3 more sources

Not All Bacterial Outer-Membrane Proteins Are β-Barrels. [PDF]

MicroPubl Biol
The discovery of Wza, an octomeric helical barrel integral bacterial outer-transmembrane protein, has challenged the widely held understanding that all integral outer-membrane proteins of Gram-negative bacteria are closed β-barrels composed of transmembrane β- strands.
Heido J, Keng S, Poyrazoglu H, Priyam E, Jayasinghe S.   +4 more
europepmc   +3 more sources

Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel [PDF]

Nature Communications, 2019
The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). Here authors develop a method to trap an E.
Matthew T. Doyle, Harris D. Bernstein
doaj   +2 more sources

Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins.

Curr Opin Struct Biol, 2015
It has until recently been unclear whether outer membrane proteins (OMPs) of Gram-negative bacteria are organized or distributed randomly. Studies now suggest promiscuous protein-protein interactions (PPIs) between β-barrel OMPs in Escherichia coli govern their local and global dynamics, engender spatiotemporal patterning of the outer membrane into ...
Kleanthous C, Rassam P, Baumann CG.
europepmc   +3 more sources

Living on the edge: Simulations of bacterial outer-membrane proteins

Biochimica Et Biophysica Acta - Biomembranes, 2016
Gram-negative bacteria are distinguished in part by a second, outer membrane surrounding them. This membrane is distinct from others, possessing an outer leaflet composed not of typical phospholipids but rather large, highly charged molecules known as lipopolysaccharides.
James C Gumbart, Karl Lundquist, Anna Pavlova   +2 more
exaly   +3 more sources