Results 61 to 70 of about 827,513 (260)

Proteinaceous determinants of surface colonization in bacteria: Bacterial adhesion and biofilm formation from a protein secretion perspective

Frontiers in Microbiology, 2013
Bacterial colonization of biotic or abiotic surfaces results from two quite distinct physiological processes, namely bacterial adhesion and biofilm formation. Broadly speaking, a biofilm is defined as the sessile development of microbial cells.
Caroline eCHAGNOT, Caroline eCHAGNOT, Mohamed Amine eZORGANI, Thierry eASTRUC, Mickael eDesvaux   +4 more
doaj   +1 more source

Bacterial Drug Efflux Proteins.

Nippon Saikingaku Zasshi, 1995
近年,数多くの薬剤排出系が様々な菌種に見いだされ,有力な化学療法剤耐性機構として認識されつつある。特に,臨床上問題視されているMRSAや緑膿菌などの多剤耐性菌に薬剤排出蛋白が多く存在することは注目すべきである。遺伝子解析により多くの排出蛋白のアミノ酸配列が明らかにされた。薬剤排出蛋白はいずれも細胞質膜に存在するが,薬剤排出のエネルギー源として,H+の電気化学的勾配を用いるものと,ATPを用いるものと2種類ある。また,構造上関連性のない多種の薬剤を認識し排出する多剤排出蛋白の存在が排出による薬剤耐性のひとつの特徴であるが,特異性の高い排出蛋白との間で一次配列の相同性が高く,多剤排出蛋白がどのように基質を認識しているのか興味が持たれる ...
SOMEYA, Yuichi, YAMAGUCHI, Akihito
openaire   +3 more sources

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The Composition of the Bacterial Culture Media Influences the Secretion and Sorting of Proteins into the Bacterial Extracellular Vesicle

Journal of Pure and Applied Microbiology
Bacterial Extracellular vesicles or BEVs are infinitesimal bi-layered lipid vesicles secreted from non-pathogenic and pathogenic bacteria to be used as a targeted drug-delivering system (DDS).
Harini Hariharan, Yasodha Kesavan, Habeeb Shaik Mohideen, Natesan Sella Raja, Subha Loganathan   +4 more
doaj   +1 more source

Exploitation of host polyubiquitination machinery through molecular mimicry by eukaryotic-like bacterial F-box effectors

Frontiers in Cellular and Infection Microbiology, 2010
Microbial pathogens have evolved exquisite mechanisms to interfere and intercept host biological processes, often through molecular mimicry of specific host proteins.
Yousef eAbu Kwaik, Christopher T. Price
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Comparison of bacterial intracellular and secreted proteins produced in milk versus medium for Escherichia coli by proteomic analysis

Journal of Dairy Science
: The growth and reproduction of microorganisms are dependent on nutrient supply. Here, milk and Luria-Bertani (LB) media were used as nutrition sources for Escherichia coli, and the changes in bacterial and secretory proteins at 3 time points (3, 9, and
Qiyue Sun, Yanxin Li, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +7 more
doaj   +1 more source

Bacterial flagellar capping proteins adopt diverse oligomeric states

eLife, 2016
Flagella are crucial for bacterial motility and pathogenesis. The flagellar capping protein (FliD) regulates filament assembly by chaperoning and sorting flagellin (FliC) proteins after they traverse the hollow filament and exit the growing flagellum tip.
Sandra Postel, Daniel Deredge, Daniel A Bonsor, Xiong Yu, Kay Diederichs, Saskia Helmsing, Aviv Vromen, Assaf Friedler, Michael Hust, Edward H Egelman, Dorothy Beckett, Patrick L Wintrode, Eric J Sundberg   +12 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source