Results 61 to 70 of about 169,837 (200)
Biotechnology and Bioengineering, EarlyView.ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner +4 more
wiley +1 more source
Molecular Systems Biology, 2012 Viral infection depends on a complex interplay between host and viral factors. Here, we link host susceptibility to viral infection to a network encompassing sulfur metabolism, tRNA modification, competitive binding, and programmed ribosomal ...
Nathaniel D Maynard +3 more
doaj +1 more source
Stochastic Cell Fate and Longevity of Offspring
Cell Journal, 2017 Objective: Cellular decision-making is a key process in which cells with similar genetic and environmental background make dissimilar decisions. This stochastic process, which happens in prokaryotic and eukaryotic cells including stem cells, causes ...
Faezeh Dorri +2 more
doaj +1 more source
Natural biased coin encoded in the genome determines cell strategy. [PDF]
PLoS ONE, 2014 Decision making at a cellular level determines different fates for isogenic cells. However, it is not yet clear how rational decisions are encoded in the genome, how they are transmitted to their offspring, and whether they evolve and become optimized ...
Faezeh Dorri +6 more
doaj +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin +3 more
wiley +1 more source
Bacteriophage Lambda as a Cloning Vector
Microbiological Reviews, 1993 [This corrects the article on p. 582 in vol. 56.].
openaire +2 more sources
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT Predicted protein structures now enable fold discovery beyond the reach of sequence homology, but scalable and interpretable methods remain limited. We present Cooper‐Beta, a resource‐efficient, chain‐resolved framework for detecting β‐barrel‐like folds from strand connectivity.
Shuyu Zhong, Yuying Jiang
wiley +1 more source
Can painting human cells with exogenous maltoporin enable efficient therapeutic gene transfer by bacteriophage lambda vectors? [PDF]
, 2010 Many gene therapy strategies require transfer of high-molecular weight DNA into human cells. To enable clinical trials, these vectors need to be produced on a large scale and at low cost.
Tolmachov, O.
core +1 more source
Nature CommunicationsA major challenge in evolutionary biology is explaining how populations navigate rugged fitness landscapes without getting trapped on local optima.
Michael B. Doud +5 more
doaj +1 more source
Programmable Assembly of Higher‐Order DNA Nanostructures from Microbial ssDNA Staple Libraries
Small, EarlyView.A phage‐free, microbial platform produces programmable circular ssDNA libraries that are enzymatically converted into DNA origami staple pools. Rolling‐circle replication generates sequence‐complex ssDNA with >99% fidelity, enabling assembly of DNA tiles and tubules and supporting nanostructure formation even in crude cellular lysates.
Ming Hung Yen +4 more
wiley +1 more source