Results 71 to 80 of about 169,837 (200)

High‐Throughput FRET Affinity Screening Technique (HTFAST) for Cell‐Free Expressed Binding Protein Characterization

Small, EarlyView.
High‐Throughput FRET Affinity Screening Technique (HTFAST) for quantitative, purification‐free affinity screening of cell‐free expressed binders. A fluorescent‐protein‐fused binder (donor) is expressed directly in crude E. coli cell‐free lysate and titrated against a dye‐labeled antigen (acceptor).
Sepehr Hejazi, Bret Lange, Kimia Noroozi, Vito Jurasic, Laura R Jarboe, Nigel F Reuel   +5 more
wiley   +1 more source

Novel bacteriophages containing a genome of another bacteriophage within their genomes [PDF]

, 2012
The work was funded by the Scottish Government’s Rural and Environment Science and Analytical Services (RESAS) DivisionA novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils.
Makarova Kira S., Koonin, Eugene V, Peter J Cock, Torrance Lesley, Kira S Makarova, Swanson, Maud M, Lesley Torrance, Torrance, Lesley, Reavy Brian, Hopkins, David W, Reavy, Brian, Hopkins, David W., Michael Taliansky, Cock, Peter J, Hopkins David W., Makarova, Kira S, Maud M Swanson, Swanson, Maud M., Makarova, Kira S., Koonin, Eugene V., Koonin Eugene V., Swanson Maud M., Taliansky, Michael, Cock Peter J., David W Hopkins, Taliansky Michael, Cock, Peter J., Brian Reavy, Eugene V Koonin   +28 more
core   +1 more source

Precision targeting of genetic variations in mixed bacterial cultures using CRISPR-Cas12a-programmed λ phages

Frontiers in Microbiology
The CRISPR-Cas system, an adaptive immune mechanism in prokaryotes against bacteriophages, has been developed into a versatile tool for recognizing and cleaving target nucleic acid sequences.
Chan Kyeong Lee, Ho Joung Lee, Song Hee Jeong, Sang Jun Lee   +3 more
doaj   +1 more source

Asymptotic r2 Enhancement in Formulation‐Optimized Lipid‐Iron Oxide Nanoparticles for Assessing Glioblastoma Heterogeneity at Ultrahigh‐Field MRI

Small, EarlyView.
Lipid‐coated superparamagnetic iron oxide nanoparticles (LSPIONs) are developed as MRI‐visible lipid nanocarriers for glioblastoma imaging. By tuning nanoparticle size and SPION loading, their transverse relaxivity (r2) can be systematically controlled over one order of magnitude, reaching 390 mm−1 s−1 at 9.4 T.
Marcos Bazarra‐Barreiros, María Teresa Abengoza‐Bello, Sabela Fernández‐Rodicio, Sara Ortega‐Espina, Lucía García‐Astray, José Castillo, Ramón Iglesias‐Rey, Pelayo García‐Acevedo, Pablo Hervella   +8 more
wiley   +1 more source

The NMR structure of the Ea22 lysogenic developmental protein from lambda bacteriophage

Scientific Reports
The ea22 gene resides in a relatively uncharacterized region of the lambda bacteriophage genome between the exo and xis genes and is among the earliest genes transcribed upon infection.
Cameron Goddard, Bożena Nejman-Faleńczyk, Logan W. Donaldson   +2 more
doaj   +1 more source

Microfluidic Molecular Diagnostic Chips: Functional Modules, Integration Strategies, and Emerging Applications

Small Methods, EarlyView.
This comprehensive review delivers an engineering‐centric analysis of microfluidic molecular diagnostic chips, breaking them down into foundational modules from fluid manipulation to bio‐sensing. Beyond chip design, it evaluates sample‐to‐answer integration strategies and commercial readiness levels.
Donghoon Koo, Egor Kokin, Sangyul Baik, Chanyong Park, Minji Kim, Jeongmin Kim, Ryungeun Song, Hosub Lim   +7 more
wiley   +1 more source

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli : Tuning inclusion body architecture and protein yield via dissolved oxygen control

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 12, Page 2550-2562, December 2026.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil, Norma A Valdez‐Cruz, Sara Restrepo‐Pineda, Nuria Sánchez‐Puig, Rafael Vázquez‐Duhalt, Rubén D Cadena‐Nava, Néstor O Pérez, Mauricio A Trujillo‐Roldán   +7 more
wiley   +1 more source

Expression of Human CYP2D6 in Artificial Cells Based on Polymer‐Lipid Hybrid Vesicles

ChemBioChem, Volume 27, Issue 21, 12 November 2026.
Expression of eGFP and human cytochrome P450 2D6 (CYP2D6) within the lumen of giant polymer‐lipid hybrid vesicles using a Leishmania tarentolae‐based cell‐free protein expression system is illustrated. Both proteins were expressed in the vesicle lumen, and low‐polymer‐content hybrid vesicles were the most suitable scaffolds for CYP2D6 expression with ...
Sarah Dahl, Paula De Dios Andres, Grégory Beaune, Jaakko V. I. Timonen, James Behrendorff, Kirill Alexandrov, Wayne Johnston, Brigitte Städler   +7 more
wiley   +1 more source

Imaging data of E. coli infected with bacteriophage lambda

, 2020
Fluorescence microscopy data (DAPI green and FM4-64 red) of E. coli JP313 cells with a plasmid-encoded CBASS system from E. coli MS115-1. pLAC22 = Empty Vector. pKDC3719 = Full CBASS system from E. coli MS115-1.
Corbett, K (via Mendeley Data), Corbett, Kevin, Kevin Corbett (8254563), Erica Birkholz (8254566)   +3 more
core   +2 more sources

The Potential Role of Gut Microbiome Dysbiosis and Functional Reprogramming in Colorectal Liver Metastasis via the Gut–Liver Axis

Cancer Reports, Volume 9, Issue 11, November 2026.
ABSTRACT Background Colorectal liver metastasis (CRLM) is a leading cause of mortality in colorectal cancer (CRC), yet current surveillance often misses occult micrometastases, underscoring an urgent need for early non‐invasive biomarkers and mechanistic insights.
Yuejin Sheng, Xiaoyu Dai, Jiaqi Lu, Hao Zhang, Ping Cai   +4 more
wiley   +1 more source