Results 31 to 40 of about 50,190 (266)

THE GROWTH OF BACTERIOPHAGE [PDF]

Journal of General Physiology, 1939
1. An anti-Escherichia coli phage has been isolated and its behavior studied. 2. A plaque counting method for this phage is described, and shown to give a number of plaques which is proportional to the phage concentration. The number of plaques is shown to be independent of agar concentration, temperature of plate incubation, and ...
Ellis, Emory L., Delbrück, Max
openaire   +3 more sources

Application of bacteriophages [PDF]

Microbiology Australia, 2017
The emergence of antibiotic-resistant bacteria and decrease in the discovery rate of novel antibiotics takes mankind back to the ‘pre-antibiotic era’ and search for alternative treatments. Bacteriophages have been one of promising alternative agents which can be utilised for medicinal and biological control purposes in agriculture and related fields ...
Aminov, Rustam, Caplin, Jonathan, Chanishvili, Nina, Coffey, Aidan, Cooper, Ian, De Vos, Daniel, Doskar, Jiri, Friman, Ville-Petri, Kurtboke, Ipek, Pantucek, Roman, Pirnay, Jean-Paul, Resch, Gregory, Rohde, Christine, Sybesma, Wilbert, Wittmann, Johannes   +14 more
openaire   +4 more sources

Ethics framework for treatment use of investigational drugs

BMC Medical Ethics, 2020
Background Expanded access is the use of investigational drugs (IDs) outside of clinical trials. Generally it is performed in patients with serious and life-threatening diseases who cannot be treated satisfactorily with authorized drugs.
Jan Borysowski, Andrzej Górski
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The Potential of Phage Therapy in Sepsis

Frontiers in Immunology, 2017
Sepsis remains a difficult clinical challenge, since our understanding of its immunopathology is incomplete and no efficacious treatment currently exists. Its earlier stage results from an uncontrolled inflammatory response to bacteria while in the later
Andrzej Górski, Andrzej Górski, Ewa Jończyk-Matysiak, Marzanna Łusiak-Szelachowska, Ryszard Międzybrodzki, Ryszard Międzybrodzki, Beata Weber-Dąbrowska, Jan Borysowski   +7 more
doaj   +1 more source

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

Frontiers in Microbiology, 2021
Vibrio parahaemolyticus is a foodborne pathogen that is frequently isolated from a variety of seafood. To control this pathogenic Vibrio spp., the implementation of bacteriophages in aquaculture and food industries have shown a promising alternative to ...
Chia Wanq Tan, Yaya Rukayadi, Hanan Hasan, Noor-Azira Abdul-Mutalib, Noor-Azira Abdul-Mutalib, Nuzul Noorahya Jambari, Nuzul Noorahya Jambari, Hirofumi Hara, Tze Young Thung, Epeng Lee, Son Radu, Son Radu   +11 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The Role of Moderate and Virulent Endogenous Bacteriophages in the Treatment of Pyoinflammatory Infections in Intensive Care Unit Patients

Неотложная медицинская помощь, 2020
. In connection with the growth of resistance of pathogens of pyoinflammatory infections (PII) to antibiotics, physicians began to use bacteriophages, which are widespread where there are homologous bacteria.
E. B. Lazareva, T. V. Chernenkaya, A. K. Shabanov, N. V. Yevdokimova, E. L. Zhilenkov, M. A. Godkov, S. S. Petrikov   +6 more
doaj   +1 more source