Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Innovating on Well Biofouling Remediation: A Bacteriophage Cocktail Approach [PDF]
Waller Creek Working ...
Bae, Sungwoo +3 more
core +1 more source
The past three years have seen an escalation in the number of sequenced bacteriophage genomes with more than 500 now in the NCBI phage database, representing a more than threefold increase since 2005. These span at least 70 different bacterial hosts, with two-thirds of the sequenced genomes of phages representing only eight bacterial hosts.
openaire +2 more sources
A dual‐layer living hydrogel, ProΦGel, integrates bacteriophages and probiotics for synergistic wound infection therapy. The outer gelatin‐based matrix releases phages on demand in response to P. aeruginosa infections, while inner alginate beads sustain probiotic delivery.
Siyuan Tao +6 more
wiley +1 more source
Klebsiella pneumoniae Phage M198 and Its Therapeutic Potential
The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including Klebsiella ...
Lika Leshkasheli +6 more
doaj +1 more source
DNA content of a functioning chicken kinetochore [PDF]
© The Author(s) 2014. In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and ...
A Alonso +39 more
core +3 more sources
Color Routing and Beam Steering of Single‐Molecule Emission with a Spherical Silicon Nanoantenna
We experimentally demonstrate broadband directional emission from single molecules using a single spherical silicon nanoparticle assembled via DNA origami. By varying nanoparticle (NP) size and emitter position, we achieve unidirectional emission, beam steering, and color routing at the nanoscale, revealing modal interference as the underlying ...
María Sanz‐Paz +9 more
wiley +1 more source
Treatability of U.S. Environmental Protection Agency Contaminant Candidate List Viruses: Removal of Coxsackievirus and Echovirus using Enhanced Coagulation [PDF]
Enhanced coagulation was evaluated for removal efficacy of coxsackievirus and echovirus (Contaminant Candidate List [CCL] enteroviruses), poliovirus, four potential surrogate bacteriophages, and dissolved organic carbon (DOC).
Abbaszadegan, Morteza +2 more
core +1 more source
Effective elimination of Staphylococcal contamination from hospital surfaces by a bacteriophage-probiotic sanitation strategy: a monocentric study. [PDF]
Persistent contamination of hospital surfaces and antimicrobial resistance (AMR) are recognized major causes of healthcare-associated infections (HAI). We recently showed that a probiotic-based sanitation (PCHS) can stably decrease surface pathogens and ...
BISI, Matteo +6 more
core +1 more source
DNA‐Origami‐Assembled Rhodium Nanoantennas for Deep‐UV Label‐Free Single‐Protein Detection
Deep‐UV autofluorescence of single proteins in DNA origami‐based rhodium nanoantennas. Precisely positioned within the plasmonic nanogap, proteins retain their native state while their intrinsic emission is strongly amplified, enabling label‐free single‐molecule detection and opening new opportunities for highly sensitive and specific biosensing ...
Nicco Corduri +8 more
wiley +1 more source

