Results 31 to 40 of about 41,285 (266)

Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

Human Genomics, 2004
Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to lower
Belousov Yevgeniy S, Welch Robert A, Sanders Silvia, Mills Alan, Kulchenko Alena, Dempcy Robert, Afonina Irina A, Walburger David K, Glaser Cynthia L, Yadavalli Sunita, Vermeulen Nicolaas MJ, Mahoney Walt   +11 more
doaj   +1 more source

Understanding Rad51 function is a prerequisite for progress in cancer research

QRB Discovery, 2020
The human protein Rad51 is double-edged in cancer contexts: on one hand, preventing tumourigenesis by eliminating potentially carcinogenic DNA damage and, on the other, promoting tumours by introducing new mutations.
Bengt Nordén, Masayuki Takahashi
doaj   +1 more source

Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers

Biosensors, 2023
The multiplexed detection of disease biomarkers is part of an ongoing effort toward improving the quality of diagnostic testing, reducing the cost of analysis, and accelerating the treatment processes.
Anisa Kaur, Roaa Mahmoud, Anoja Megalathan, Sydney Pettit, Soma Dhakal   +4 more
doaj   +1 more source

Influence of pH on the conformation and stability of mismatch base-pairs in DNA

Journal of Molecular Biology, 1990
A series of self-complementary dodecanucleotide duplexes containing two symmetrically disposed mismatches have been studied by pH-dependent, ultraviolet light melting techniques. The results indicate that A.C, and C.C mismatches are strongly stabilized by protonation and that the degree of stabilization of the A.C mismatch depends greatly on the ...
Brown, T, Leonard, G, Booth, E, Kneale, G   +3 more
openaire   +3 more sources

Highly sensitive and selective mercury sensor based on mismatched base pairing with dioxT [PDF]

Chemical Communications, 2019
A highly selective and sensitive sensor for mercury was designed based on a new fluorescent nucleobase, dioxT.
Ji Hoon Han, Shingo Hirashima, Soyoung Park, Hiroshi Sugiyama   +3 more
openaire   +2 more sources

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system. [PDF]

The EMBO Journal, 1986
The Escherichia coli mismatch repair system does not recognize and/or repair all mismatched base pairs with equal efficiency: whereas transition mismatches (G X T and A X C) are well repaired, the repair of some transversion mismatches (e.g. A X G or C X T) appears to depend on their position in heteroduplex DNA of phage lambda.
G V, Fazakerley, E, Quignard, A, Woisard, W, Guschlbauer, G A, van der Marel, J H, van Boom, M, Jones, M, Radman   +7 more
openaire   +2 more sources

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

Molecular Dynamics Simulation of Melting of the DNA Duplex with Silver-Mediated Cytosine–Cytosine Base Pair

Computation
Metal-mediated base pairs in DNA double helix molecules open up broad opportunities for biosensors based on DNA clusters with silver due to their low toxicity and applicability in drug design.
Elena B. Gusarova, Natalya A. Kovaleva
doaj   +1 more source

Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs [PDF]

Nucleic Acids Research, 1987
The dissociation kinetics of 19 base paired oligonucleotide-DNA duplex containing a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount ...
S, Ikuta, K, Takagi, R B, Wallace, K, Itakura   +3 more
openaire   +2 more sources