Results 51 to 60 of about 168,088 (301)
PRISE2: software for designing sequence-selective PCR primers and probes. [PDF]
, 2014 BackgroundPRISE2 is a new software tool for designing sequence-selective PCR primers and probes. To achieve high level of selectivity, PRISE2 allows the user to specify a collection of target sequences that the primers are supposed to amplify, as well as
Borneman, James +3 more
core +2 more sources
Molecular Oncology, EarlyView.Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova +25 more
wiley +1 more source
Human Genomics, 2004 Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to lower
Belousov Yevgeniy S +11 more
doaj +1 more source
Understanding Rad51 function is a prerequisite for progress in cancer research
QRB Discovery, 2020 The human protein Rad51 is double-edged in cancer contexts: on one hand, preventing tumourigenesis by eliminating potentially carcinogenic DNA damage and, on the other, promoting tumours by introducing new mutations.
Bengt Nordén, Masayuki Takahashi
doaj +1 more source
Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers
Biosensors, 2023 The multiplexed detection of disease biomarkers is part of an ongoing effort toward improving the quality of diagnostic testing, reducing the cost of analysis, and accelerating the treatment processes.
Anisa Kaur +4 more
doaj +1 more source
Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer
Molecular Oncology, EarlyView.COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann +61 more
wiley +1 more source
Repair of defined single base-pair mismatches in Escherichia coli [PDF]
Proceedings of the National Academy of Sciences, 1985 Heteroduplexes with single base-pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage λ DNA and used to transfect Escherichia coli . Each of the eight possible single base-pair mismatches was constructed.
C, Dohet, R, Wagner, M, Radman
openaire +2 more sources
The Error and Repair Catastrophes: A Two-Dimensional Phase Diagram in the Quasispecies Model
, 2003 This paper develops a two gene, single fitness peak model for determining the equilibrium distribution of genotypes in a unicellular population which is capable of genetic damage repair.
A. Giraud +29 more
core +1 more source
Molecular Oncology, EarlyView.Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena +15 more
wiley +1 more source
ComputationMetal-mediated base pairs in DNA double helix molecules open up broad opportunities for biosensors based on DNA clusters with silver due to their low toxicity and applicability in drug design.
Elena B. Gusarova, Natalya A. Kovaleva
doaj +1 more source