Results 41 to 50 of about 41,285 (266)
Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis
Molecular Oncology, EarlyView.This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht +3 more
wiley +1 more source
Dependence of nucleosome mechanical stability on DNA mismatches
eLifeThe organization of nucleosomes into chromatin and their accessibility are shaped by local DNA mechanics. Conversely, nucleosome positions shape genetic variations, which may originate from mismatches during replication and chemical modification of DNA ...
Thuy TM Ngo +5 more
doaj +1 more source
Mismatch repair in recombination of bacteriophage T4
Biomolecular Concepts, 2012 The review focuses on the mechanism of mismatch repair in bacteriophage T4. It was first observed in T4 as an extra recombination mechanism, which contributed to the general recombination only when particular rII mutations were used as genetic markers ...
Shcherbakov Victor P.
doaj +1 more source
DNA Polymerase Inhibition by High Kinetic Stability of T-HgII-T Base Pairs
CHIMIA, 2017 A fluorescent surrogate of thymidine called DMAT was used for the first fluorescence-based study of HgII binding to discrete T-T sites in duplex DNA.
Olivia P. Schmidt
doaj +1 more source
Cellular Target of a Rhodium Metalloinsertor is the DNA Base Pair Mismatch
Chemistry – A European Journal, 2019 AbstractDefects in DNA mismatch repair (MMR) are commonly found in various cancers, especially in colorectal cancers. Despite the high prevalence of MMR‐deficient cancers, mismatch‐targeted therapeutics are limited and diagnostic tools are indirect. Here, we examine the cytotoxic properties of a rhodium metalloinsertor, [Rh(phen)(chrysi)(PPO)]2+ (RhPPO)
Kelsey M. Boyle +3 more
openaire +4 more sources
Selective oxidation of methylcytosine in single base pair mismatches by osmium complex [PDF]
Nucleic Acids Symposium Series, 2007 We have reported a novel method for methylcytosine (M)-positive epigenotyping, in which an osmium ion site-specifically binds to M in a single strand or a bulge structure of DNA on oxidation of an M C5-C6 double bond. Herein, we have examined the osmium oxidation reactivity of M that formed a mismatched base pair with various bases, and observed its ...
Akiko, Nomura +2 more
openaire +2 more sources
Molecular Oncology, EarlyView.Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim +3 more
wiley +1 more source
Screening of anomalies in a double-stranded DNA via global electrical measurements
AIP AdvancesWe conduct two-terminal charge transport calculations to detect biological defects in three types of DNA sequences: periodic, quasi-periodic and random.
Sourav Kundu
doaj +1 more source
DNA functionalization by dynamic chemistry
Beilstein Journal of Organic Chemistry, 2016 Dynamic combinatorial chemistry (DCC) is an attractive method to efficiently generate libraries of molecules from simpler building blocks by reversible reactions under thermodynamic control.
Zeynep Kanlidere +3 more
doaj +1 more source
Population genetic structure of Marbled Rockfish, Sebastiscus marmoratus (Cuvier, 1829), in the northwestern Pacific Ocean [PDF]
ZooKeys, 2019 Sebastiscus marmoratus is an ovoviviparous fish widely distributed in the northwestern Pacific. To examine the gene flow and test larval dispersal strategy of S.
Lu Liu +6 more
doaj +3 more sources