Results 61 to 70 of about 40,087 (166)

Real-Time PCR Assay for Quantitative Mismatch Detection

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
doaj   +1 more source

Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs

Analytical Sciences, 2007
Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex ...
Kosuke, Mukumoto, Sadayoshi, Watanabe, Takahiko, Nojima, Michinori, Waki, Shigeori, Takenaka   +4 more
openaire   +3 more sources

Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction

Sultan Qaboos University Medical Journal, 2000
Objective and Method – To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3•– end of a primer to amplify a 268 bp (base pair) region of the human •–globin gene using different annealing temperatures (45 to 65•C).
Simsek M, Adnan H
doaj  

A Paper-Based Sandwich Format Hybridization Assay for Unlabeled Nucleic Acid Detection Using Upconversion Nanoparticles as Energy Donors in Luminescence Resonance Energy Transfer

Nanomaterials, 2015
Bioassays based on cellulose paper substrates are gaining increasing popularity for the development of field portable and low-cost diagnostic applications.
Feng Zhou, M. Omair Noor, Ulrich J. Krull   +2 more
doaj   +1 more source

Studies of the structure and stability of base pair mismatches, base pairs involving modified bases, and DNA-drug complexes

Journal of Chemical Crystallography, 1994
Of the DNA bases, the hydrogen bonding characteristics of guanine (G) are complementary only to those of cytidine (C) and, similarly, the hydrogen bonding characteristics of adenine (A) are complementary only to those of thymine (T) (Fig. 1). This is the fundamental basis of Watson-Crick base pairing in DNA and ensures the extremely high fidelity of ...
Leonard, G, Hunter, W, Brown, T
openaire   +2 more sources

Unraveling mutagenic processes influencing the tumor mutational patterns of individuals with constitutional mismatch repair deficiency

Nature Communications
Constitutional mismatch repair deficiency (CMMRD), caused by bi-allelic germline variants in mismatch repair (MMR) genes, is associated with high cancer incidence early in life.
Dilys D. Weijers, Snežana Hinić, Emma Kroeze, Mark AJ Gorris, Gerty Schreibelt, Sjors Middelkamp, Arjen R. Mensenkamp, Reno Bladergroen, Kiek Verrijp, Nicoline Hoogerbrugge, Pieter Wesseling, Rachel S. van der Post, Jan LC Loeffen, Corrie EM Gidding, Mariëtte CA van Kouwen, I. Jolanda M. de Vries, Ruben van Boxtel, Richarda M. de Voer, Marjolijn CJ Jongmans, Roland P. Kuiper   +19 more
doaj   +1 more source

Muver, a computational framework for accurately calling accumulated mutations

BMC Genomics, 2018
Background Identification of mutations from next-generation sequencing data typically requires a balance between sensitivity and accuracy. This is particularly true of DNA insertions and deletions (indels), that can impart significant phenotypic ...
Adam B. Burkholder, Scott A. Lujan, Christopher A. Lavender, Sara A. Grimm, Thomas A. Kunkel, David C. Fargo   +5 more
doaj   +1 more source

High fidelity DNA ligation prevents single base insertions in the yeast genome

Nature Communications
Finalization of eukaryotic nuclear DNA replication relies on DNA ligase 1 (LIG1) to seal DNA nicks generated during Okazaki Fragment Maturation (OFM). Using a mutational reporter in Saccharomyces cerevisiae, we previously showed that mutation of the high-
Jessica S. Williams, Scott. A. Lujan, Mercedes E. Arana, Adam B. Burkholder, Percy P. Tumbale, R. Scott Williams, Thomas A. Kunkel   +6 more
doaj   +1 more source

RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

BMC Biotechnology, 2011
Background The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings.
Powers Kristy M, Rupp Susan M, Beltz Kristin R, Rose Scott D, Dobosy Joseph R, Behlke Mark A, Walder Joseph A   +6 more
doaj   +1 more source

Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

Beilstein Journal of Nanotechnology, 2016
Background: DNA hybridization is at the basis of most current technologies for genotyping and sequencing, due to the unique properties of DNA base-pairing that guarantee a high grade of selectivity.
Maryse D. Nkoua Ngavouka, Pietro Capaldo, Elena Ambrosetti, Giacinto Scoles, Loredana Casalis, Pietro Parisse   +5 more
doaj   +1 more source