Results 71 to 80 of about 842,010 (246)

Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations.

PLoS ONE, 2020
The programmable sequence specificity of CRISPR has found uses in gene editing and diagnostics. This manuscript describes an additional application of CRISPR through a family of novel DNA enrichment technologies.
Jennifer L Steele, Richard C Stevens, Oscar A Cabrera, Gary J Bassill, Sabrina M Cramer, Felipe Guzman, Anthony P Shuber   +6 more
doaj   +1 more source

Aβ42 promotes the aggregation of α‐synuclein splice isoforms via heterogeneous nucleation

FEBS Letters, EarlyView.
The aggregation of amyloid‐β (Aβ) and α‐synuclein (αSyn) is associated with Alzheimer's and Parkinson's diseases. This study reveals that Aβ aggregates serve as potent nucleation sites for the aggregation of αSyn and its splice isoforms, shedding light on the intricate interplay between these two pathogenic proteins.
Alexander Röntgen, Zenon Toprakcioglu, Michele Vendruscolo   +2 more
wiley   +1 more source

On the Fuzzy Convergence

Journal of Applied Mathematics, 2011
The aim of this paper is to introduce and study the fuzzy neighborhood, the limit fuzzy number, the convergent fuzzy sequence, the bounded fuzzy sequence, and the Cauchy fuzzy sequence on the base which is adopted by Abdul Hameed (every real number r is ...
Abdul Hameed Q. A. Al-Tai
doaj   +1 more source

Low Base-Substitution Mutation Rate but High Rate of Slippage Mutations in the Sequence Repeat-Rich Genome of Dictyostelium discoideum

G3: Genes, Genomes, Genetics, 2020
We describe the rate and spectrum of spontaneous mutations for the social amoeba Dictyostelium discoideum, a key model organism in molecular, cellular, evolutionary and developmental biology. Whole-genome sequencing of 37 mutation accumulation lines of D.
Sibel Kucukyildirim, Megan Behringer, Way Sung, Debra A. Brock, Thomas G. Doak, Hatice Mergen, David C. Queller, Joan E. Strassmann, Michael Lynch   +8 more
doaj   +1 more source

ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling

FEBS Letters, EarlyView.
In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li, Gerard van der Zon, Peter ten Dijke, Tong Shen   +3 more
wiley   +1 more source

Almost multiphase sequences based on Chu sequences

Electronics Letters, 2015
A new family of almost multiphase sequences (AMPSs) with perfect periodic autocorrelation function (PACF) is offered. The obtained sequences are based on Chu sequences and differ from them by the structure, the peak‐factor which is nearly equal to one, and by fewer phases for the same periods.
V.E. Gantmakher, M.V. Zaleshin
openaire   +2 more sources

Application of Sequence Embedding in Protein Sequence-Based Predictions

, 2022
In sequence-based predictions, conventionally an input sequence is represented by a multiple sequence alignment (MSA) or a representation derived from MSA, such as a position-specific scoring matrix. Recently, inspired by the development in natural language processing, several applications of sequence embedding have been observed.
Ibtehaz, Nabil, Kihara, Daisuke
openaire   +2 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

A repetitive sequence assembler based on next-generation sequencing

Genetics and Molecular Research, 2016
Repetitive sequences of variable length are common in almost all eukaryotic genomes, and most of them are presumed to have important biomedical functions and can cause genomic instability. Next-generation sequencing (NGS) technologies provide the possibility of identifying capturing these repetitive sequences directly from the NGS data.
Y Wang, Y Tu, Xinwu Chen, Shuaibin Lian, Ling-Ling Wang   +4 more
openaire   +3 more sources

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source