Results 41 to 50 of about 20,525 (171)

Label‐Free and Immobilization‐Free Protein‐Binding Assays by Ultraviolet Transient Absorption Microscopy

Advanced Science, EarlyView.
A femtosecond UV pump–probe microscope resolves binding‐induced changes in protein excited‐state dynamics and converts these intrinsic contrasts into an equilibrium affinity readout. By probing tryptophan responses directly in solution, the approach enables quantitative determination of dissociation constants without labeling or immobilization ...
Jianghao Shen, Qiangqiang Wang, Fan Wu, Yang Gao, Lu Lan, Pu Wang   +5 more
wiley   +1 more source

Accelerating Primary Screening of USP8 Inhibitors from Drug Repurposing Databases with Tree‐Based Machine Learning

Advanced Intelligent Discovery, EarlyView.
This study introduces a tree‐based machine learning approach to accelerate USP8 inhibitor discovery. The best‐performing model identified 100 high‐confidence repurposable compounds, half already approved or in clinical trials, and uncovered novel scaffolds not previously studied. These findings offer a solid foundation for rapid experimental follow‐up,
Yik Kwong Ng, Sharon Ai Er Lam, Wei Chun Lim, Hannah Hui Min Toh, Shi Jun Ang   +4 more
wiley   +1 more source

Dissecting the Effect of Berberine on the Intestinal Microbiome in the Weaned Piglets by Metagenomic Sequencing

Frontiers in Microbiology, 2022
This study aimed to investigate the microbial structure and function in the rectum of weaned piglets with berberine supplementation. Twelve healthy 21-day-old Duorc × (Landrace × Large White) weaned piglets (similar body weight) were evenly divided into ...
Hong Hu, Kexing Xu, Kunping Wang, Feng Zhang, Feng Zhang, Xi Bai   +5 more
doaj   +1 more source

Heat Stress in Quail: Impacts on Health and Productivity, and Mitigation Strategies

Animal Research and One Health, EarlyView.
Heat stress disrupts physiological homeostasis in quail, inducing oxidative stress, immune dysregulation, and metabolic imbalance, which impair growth, reproduction, product quality, and welfare. Integrating nutritional, environmental, and genetic–epigenetic strategies enhances thermotolerance, sustains productivity, and supports climate‐smart quail ...
T. A. Eletu, I. A. Anjola, L. O. Fasasi, D. P. Fadipe, A. T. Afuye, F. O. Fasanya, O. E. Oke   +6 more
wiley   +1 more source

Berberine Ameliorates Subarachnoid Hemorrhage Injury via Induction of Sirtuin 1 and Inhibiting HMGB1/Nf-κB Pathway

Frontiers in Pharmacology, 2020
Excessive cerebral inflammation plays a key role in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Berberine, an isoquinoline alkaloid isolated from Chinese herb Coptis chinensis, possesses anti-inflammatory, and neuroprotective effects ...
Xiang-Hua Zhang, Lei Peng, Jing Zhang, Yi-Peng Dong, Cheng-Jun Wang, Cang Liu, Da-Yong Xia, Xiang-Sheng Zhang   +7 more
doaj   +1 more source

Chemoproteomics reveals berberine directly binds to PKM2 to inhibit the progression of colorectal cancer

iScience, 2022
Summary: Colorectal cancer is one of the most serious tumors and berberine can inhibit the recurrence and transformation of colorectal adenoma into colorectal cancer.
Shi-Hai Yan, Li-Mu Hu, Xue-Hui Hao, Jiang Liu, Xi-Ying Tan, Zhi-Rong Geng, Jing Ma, Zhi-Lin Wang   +7 more
doaj   +1 more source

New opportunities for bioscaffold‐enabled spinal cord injury repair

BMEMat, EarlyView.
Schematic illustration of bioscaffolds for spinal cord injury repair. We summarize the effects of bioscaffold properties on SCI repair, highlight different types of bioscaffolds, various fabrication strategies, and in vivo transformations for the clinical development of SCI‐repairing bioscaffolds.
Xiaoqing Qi, Yicheng Fu, Zhaoliang Su, Li Li, Subrata Chakrabarti, Peng Li, Yilun Wu, Fang Liu, Teng Gao, Zhifeng Dong, Lei Liu, Pei Cao   +11 more
wiley   +1 more source

Berberine Improves Intestinal Motility and Visceral Pain in the Mouse Models Mimicking Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D) Symptoms in an Opioid-Receptor Dependent Manner.

PLoS ONE, 2015
Background and aimsBerberine and its derivatives display potent analgesic, anti-inflammatory and anticancer activity. Here we aimed at characterizing the mechanism of action of berberine in the gastrointestinal (GI) tract and cortical neurons using ...
Chunqiu Chen, Meiling Lu, Qiuhui Pan, Jakub Fichna, Lijun Zheng, Kesheng Wang, Zhen Yu, Yongyu Li, Kun Li, Aihong Song, Zhongchen Liu, Zhenshun Song, Martin Kreis   +12 more
doaj   +1 more source

In Vitro Assessment of Berberine against Ichthyophthirius multifiliis in Goldfish

Pathogens, 2022
Ichthyophthirius multifiliis is a pathogenic ciliate parasite, which infects almost all freshwater teleost fish and leads to significant economic losses.
Ke Huang, Guangran Hu, Runqiu Wang, Qingwen Zeng, Wenxiang Li, Hong Zou, Shangong Wu, Guitang Wang, Ming Li   +8 more
doaj   +1 more source

Deciphering the catalytic and pharmacological mechanisms of Coptis chinensis herbzymes to renovate intestinal microenvironment for colitis alleviation

BMEMat, EarlyView.
The synthesized CCzymes possess both antioxidant enzyme activity and pharmacological properties inherent to Coptis chinensis. By their antioxidant enzyme activity, CCzymes can attenuate oxidative stress within the inflammatory region of ulcerative colitis (UC), while their pharmacological activity acts on macrophage polarization and the intestinal ...
Zhichao Deng, Can Li, Yujie Zhang, Yuanyuan Zhu, Chenxi Xu, Bowen Gao, Mingxin Zhang, Martina H. Stenzel, Kelong Fan, Mingzhen Zhang, Yanyan Jiang   +10 more
wiley   +1 more source