Results 11 to 20 of about 253,228 (248)

Competitive ELISA for Protein-lipopolysaccharide (LPS) Binding

Bio-Protocol, 2014
Lipopolysaccharide is the major constituent of the outer membrane of gram-negative bacteria and, once released from the bacterial surface into the bloodstream, is a potent activator of the host immune system, which can lead to septic shock.
Victoria Martínez-Sernández, Florencio M Ubeira   +1 more
doaj   +1 more source

Discovery and Characterization of a Cryptic Secondary Binding Site in the Molecular Chaperone HSP70

Molecules, 2022
Heat Shock Protein 70s (HSP70s) are key molecular chaperones that are overexpressed in many cancers and often associated with metastasis and poor prognosis. It has proven difficult to develop ATP-competitive, drug-like small molecule inhibitors of HSP70s
Suzanne O’Connor, Yann-Vaï Le Bihan, Isaac M. Westwood, Manjuan Liu, Oi Wei Mak, Gabriel Zazeri, Ana P. R. Povinelli, Alan M. Jones, Rob van Montfort, Jóhannes Reynisson, Ian Collins   +10 more
doaj   +1 more source

Involvement of the DNA Phosphorothioation System in TorR Binding and Anaerobic TMAO Respiration in Salmonella enterica

mBio, 2022
Although the phosphorothioate (PT) modification, in which the nonbridging oxygen in the DNA sugar-phosphate backbone is replaced by sulfur, has been reported to play versatile roles in multiple cellular processes, very little data have been obtained to ...
You Tang, Yue Hong, Li Liu, Xuan Du, Yaqi Ren, Susu Jiang, Wanjing Liu, Chen Chao, Zixin Deng, Lianrong Wang, Shi Chen   +10 more
doaj   +1 more source

Competitive interactions of ligands and macromolecular crowders with maltose binding protein. [PDF]

PLoS ONE, 2013
Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules.
Andrew C Miklos, Matthew Sumpter, Huan-Xiang Zhou   +2 more
doaj   +1 more source

Discrimination of Oviposition Deterrent Volatile β-Ionone by Odorant-Binding Proteins 1 and 4 in the Whitefly Bemisia tabaci

Biomolecules, 2019
: The whitefly, Bemisia tabaci, is an important invasive economic pest of agricultural crops worldwide. β-ionone has a significant oviposition repellent effect against B. tabaci, but the olfactory molecular mechanism of this insect for recognizing &#
Fengqi Li, Du Li, Youssef Dewer, Cheng Qu, Zhen Yang, Jiahui Tian, Chen Luo   +6 more
doaj   +1 more source

Thermodynamics of competitive molecular channel transport: application to artificial nuclear pores. [PDF]

PLoS ONE, 2010
In an analytical model channel transport is analyzed as a function of key parameters, determining efficiency and selectivity of particle transport in a competitive molecular environment.
Wolfgang R Bauer, Walter Nadler
doaj   +1 more source

Crystal structure of death-associated protein kinase 1 in complex with the dietary compound resveratrol

IUCrJ, 2021
Death-associated protein kinase 1 (DAPK1) is a large multidomain protein with an N-terminal serine/threonine protein kinase domain. DAPK1 is considered to be a promising molecular target for the treatment of Alzheimer's disease (AD). In the present study,
Takeshi Yokoyama, Ryoya Suzuki, Mineyuki Mizuguchi   +2 more
doaj   +1 more source

Competitive Carboxylate–Silicate Binding at Iron Oxyhydroxide Surfaces [PDF]

Langmuir, 2021
Dissolved silicate ions in wet and dry soils can determine the fate of organic contaminants via competitive binding. While fundamental surface science studies have advanced knowledge of binding in competitive systems, little is still known about the ranges of solution conditions, the time dependence, and the molecular processes controlling competitive ...
Cheng, Wei, Marsac, Remi, Hanna, Khalil, Boily, Jean-François   +3 more
openaire   +5 more sources

A Library Screening Strategy Combining the Concepts of MS Binding Assays and Affinity Selection Mass Spectrometry

Frontiers in Chemistry, 2019
The primary objective of early drug development is to identify hits and leads for a target of interest. To achieve this aim, rapid, and reliable screening techniques for a huge number of compounds are needed.
Jürgen Gabriel, Georg Höfner, Klaus T. Wanner   +2 more
doaj   +1 more source

Establishment of competitive binding assay to detect and differentiate hepatitis E virus infection

Annals of Hepatology, 2019
Introduction and Objectives: This study was undertaken to demonstrate a promising approach for detection and differentiation the serum immunoglobulin G (IgG) against hepatitis E virus (anti-HEV IgG) using a competitive binding assay established with ...
Jiyue Wen, Weizhuo Lu, Jihong Meng
doaj   +1 more source