Results 211 to 220 of about 8,224,245 (384)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase

open access: yesFEBS Open Bio, EarlyView.
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni   +4 more
wiley   +1 more source

InDeepNet: a web platform for predicting functional binding sites in proteins using InDeep. [PDF]

open access: yesNucleic Acids Res
Mareuil F   +6 more
europepmc   +1 more source

The nuclear envelope. The major site of insulin binding in rat liver nuclei.

open access: hybrid, 1978
Riccardo Vigneri   +4 more
openalex   +1 more source

Assessing computational tools for the discovery of transcription factor binding sites

open access: yesNature Biotechnology, 2005
M. Tompa   +24 more
semanticscholar   +1 more source

Characterization of the thermophilic xylanase Fsa02490Xyn from the hyperthermophile Fervidibacter sacchari belonging to glycoside hydrolase family 10

open access: yesFEBS Open Bio, EarlyView.
Fervidibacter sacchari is a member of the bacterial phylum Armatimonadota, only a few of which have been cultivated. It is a hyperthermophilic polysaccharide degradation specialist. We show that a secreted enzyme, Fsa02490Xyn, is an endoxylanase belonging to glycoside hydrolase family 10.
Nicole Torosian   +10 more
wiley   +1 more source

Identification of druggable binding sites and small molecules as modulators of TMC1. [PDF]

open access: yesCommun Biol
De-la-Torre P   +8 more
europepmc   +1 more source

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