SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Accurate prediction of protein-ATP binding sites based on a protein pretrained large language model and a fractional-order convolutional neural network. [PDF]
Sci RepGuo M, Tu Y, Yu J, Wang Y.
europepmc +1 more source
Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. [PDF]
, 1993 Stephen Morris +4 more
openalex +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
FEBS Open Bio, EarlyView.Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
RNA language model and graph attention network for RNA and small molecule binding sites prediction. [PDF]
BioinformaticsSun S, Yang J, Gao L, Li P, Liu Y.
europepmc +1 more source
A structural model of the tetrodotoxin and saxitoxin binding site of the Na+ channel
, 1994 Gregory M. Lipkind, Harry A. Fozzard
openalex +2 more sources
Identification of Cell-binding Sites on the Laminin α5 N-terminal Domain by Site-directed Mutagenesis [PDF]
, 2001 Peter Kresten Nielsen, Yoshihiko Yamada
openalex +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
FEBS Open Bio, EarlyView.Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
wiley +1 more source
OptimDase: An Algorithm for Predicting DNA Binding Sites with Combined Feature Encoding. [PDF]
Interdiscip SciLiu Z +7 more
europepmc +1 more source