Results 21 to 30 of about 225,988 (282)

Thin-Film Nanocomposite Reverse Osmosis Membrane with High Flux and Antifouling Performance via Incorporating Maleic Anhydride-Grafted Graphene Oxide

Advances in Polymer Technology, 2022
Water flux is one of the most important performance parameters of the reverse osmosis (RO) membrane. The higher water flux means lower energy cost when treating the same volume of feed solution with membrane separation technology.
Zhongyan Chen, Boqun Zhao, Yuhang Qin, Yi Zhou, Yinghua Lu, Wenyao Shao   +5 more
doaj   +1 more source

Spotting priming-active compounds using parsley cell cultures in microtiter plates

BMC Plant Biology, 2023
Background Conventional crop protection has major drawbacks, such as developing pest and pathogen insensitivity to pesticides and low environmental compatibility. Therefore, alternative crop protection strategies are needed. One promising approach treats
Kyra Hoffmann, Jana Viola Schilling, Georg Wandrey, Tim Welters, Stefan Mahr, Uwe Conrath, Jochen Büchs   +6 more
doaj   +1 more source

Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Scientific Reports, 2022
Bovine enterokinase light chain (EKL) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals.
Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby   +6 more
doaj   +1 more source

Improving Haemophilus influenzae Type b Polysaccharide Productivity Through Continuous Culture for Pentavalent Vaccine Manufacturing

Fermentation
Haemophilus influenzae type b (Hib) is a Gram-negative bacterium that causes severe infections in children under five, especially in developing countries.
Lucas Santos Solidade, Lucas Dias Vieira, Mickie Takagi   +2 more
doaj   +1 more source

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Microbial Cell Factories, 2018
Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs   +6 more
doaj   +1 more source

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

Microbial Cell Factories, 2017
Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip, K. Meier, D. Kern, J. Goldmanns, F. Stockmeier, C. Bähr, J. Büchs   +6 more
doaj   +1 more source

Highly efficient fermentation of 5-keto-d-fructose with Gluconobacter oxydans at different scales

Microbial Cell Factories, 2022
Background The global market for sweeteners is increasing, and the food industry is constantly looking for new low-caloric sweeteners. The natural sweetener 5-keto-d-fructose is one such candidate.
Svenja Battling, Tobias Engel, Elena Herweg, Paul-Joachim Niehoff, Matthias Pesch, Theresa Scholand, Marie Schöpping, Nina Sonntag, Jochen Büchs   +8 more
doaj   +1 more source

4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase in malaria parasites and enhances atovaquone efficacy

FEBS Letters, EarlyView.
Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer, Matheus Felipe Santos, Maurício Mazzine Filho, Gabriela Oliveira Castro, Agustín Hernández, Manoel Aparecido Peres, Alejandro Miguel Katzin, Marcell Crispim   +7 more
wiley   +1 more source

Chinese herbal Jin-Ying-Tang attenuates the inflammatory response by inhibiting the activation of TLR4/MyD88/TRAF-6/NIK pathway at the mRNA level in LPS-stimulated mouse mammary epithelial cells

Journal of Veterinary Research, 2016
Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined.
Yi Qiong, Li Xin, Li Yuan-Fang, Yang Hang, Zhang Xiao-Yi, Ma Zhe, Wang Lu   +6 more
doaj   +1 more source

Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates

BMC Biotechnology, 2023
Background One critical parameter in microbial cultivations is the composition of the cultivation medium. Nowadays, the application of chemically defined media increases, due to a more defined and reproducible fermentation performance than in complex ...
Jennifer Goldmanns, Georg Andreas Röhling, Marie Kristine Lipa, Theresa Scholand, Alexander Deitert, Tobias May, Evangeline Priya Haas, Matthias Boy, Andrea Herold, Jochen Büchs   +9 more
doaj   +1 more source