Results 21 to 30 of about 532,110 (332)

Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Scientific Reports, 2022
Bovine enterokinase light chain (EKL) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals.
Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby   +6 more
doaj   +1 more source

Rational Enzyme Engineering Through Biophysical and Biochemical Modeling [PDF]

, 2016
Due to its importance in the pharmaceutical industry, ligand dynamic simulations have experienced a great expansion. Using all-atom models and cutting edge hardware, one can perform non-biased ligand migration, active site search and binding studies.
Acebes, Sandra, Almendral, David, Fernández-Fueyo, Elena, Guallar, Víctor, Lucas, Fatima, Lund, Henrik, Martínez, Ángel T., Monza, Emanuele, Ruiz-Dueñas, Fracisco J.   +8 more
core   +2 more sources

Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer in Shewanella oneidensis

iScience, 2022
Summary: Bio-electrochemical systems are based on extracellular electron transfer (EET), whose efficiency relates to the expression level of numerous genes.
Yaru Chen, Meijie Cheng, Hao Song, Yingxiu Cao   +3 more
doaj  

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Microbial Cell Factories, 2018
Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs   +6 more
doaj   +1 more source

Highly efficient fermentation of 5-keto-d-fructose with Gluconobacter oxydans at different scales

Microbial Cell Factories, 2022
Background The global market for sweeteners is increasing, and the food industry is constantly looking for new low-caloric sweeteners. The natural sweetener 5-keto-d-fructose is one such candidate.
Svenja Battling, Tobias Engel, Elena Herweg, Paul-Joachim Niehoff, Matthias Pesch, Theresa Scholand, Marie Schöpping, Nina Sonntag, Jochen Büchs   +8 more
doaj   +1 more source

Optical control of the β2-adrenergic receptor with opto-prop-2: A cis-active azobenzene analog of propranolol

iScience, 2022
Summary: In this study, we synthesized and evaluated new photoswitchable ligands for the beta-adrenergic receptors β1-AR and β2-AR, applying an azologization strategy to the first-generation beta-blocker propranolol. The resulting compounds (Opto-prop-1,
Reggie Bosma, Nicola C. Dijon, Yang Zheng, Hannes Schihada, Niels J. Hauwert, Shuang Shi, Marta Arimont, Rick Riemens, Hans Custers, Andrea van de Stolpe, Henry F. Vischer, Maikel Wijtmans, Nicholas D. Holliday, Diederik W.D. Kuster, Rob Leurs   +14 more
doaj  

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

Microbial Cell Factories, 2017
Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip, K. Meier, D. Kern, J. Goldmanns, F. Stockmeier, C. Bähr, J. Büchs   +6 more
doaj   +1 more source

Interaction and Mechanism of Probiotics with Gut Flora and Immune Regulation: A Review

Shipin gongye ke-ji, 2022
Probiotics play beneficial roles in improving the host intestinal microecological balance and improving the host health level and health state. It mainly affects the host health by regulating the intestinal microbiota and participating in the immune ...
Manyu TANG, Wanqing WANG, Jingwen QIANG, Wei HUA, Shuang WU, Yali LI, Xin ZHEN, Chungeng LI, Yanling CHENG   +8 more
doaj   +1 more source

Biochemical engineering’s grand adventure

Chemical Engineering Science, 2017
Building on the recent revolution in molecular biology, enabling a wealth of bio-product innovations made from renewable feedstocks, the biotechnology field is in a transition phase to bring the products to the market. This requires a shift from natural sciences to engineering sciences with first conception of new, efficient large-scale bioprocess ...
Henk J. Noorman, Henk J. Noorman, Joseph J. Heijnen   +2 more
openaire   +4 more sources

An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics [PDF]

, 2016
The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside ...
De Sutter, Delphine, Eyckerman, Sven, Gevaert, Kris, Gonnelli, Giulia, Martens, Lennart, Samyn, Noortje, Staes, An, Timmerman, Evy, Vandemoortele, Giel, Vandermarliere, Elien   +9 more
core   +2 more sources