Results 1 to 10 of about 66,620 (264)

Gαs slow conformational transition upon GTP binding and a novel Gαs regulator

iScience, 2023
Summary: G proteins are major signaling partners for G protein-coupled receptors (GPCRs). Although stepwise structural changes during GPCR–G protein complex formation and guanosine diphosphate (GDP) release have been reported, no information is available
Donghoon Ahn, Davide Provasi, Nguyen Minh Duc, Jun Xu, Leslie Salas-Estrada, Aleksandar Spasic, Min Woo Yun, Juyeong Kang, Dongmin Gim, Jaecheol Lee, Yang Du, Marta Filizola, Ka Young Chung   +12 more
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In honor of Hartmut Karl Lichtenthaler

Photosynthetica, 2021
We honor here Hartmut Karl Lichtenthaler, a pioneer of plant physiology, plant biochemistry, plant biophysics, plant molecular biology, and stress physiology.
G. GOVINDJEE
doaj   +1 more source

Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates

STAR Protocols, 2022
Summary: Heat stress triggers a specific set of proteins in budding yeast to form solid-like biomolecular condensates, which are dispersed by molecular chaperones.
Haneul Yoo, D. Allan Drummond
doaj   +1 more source

Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing

STAR Protocols, 2023
Summary: A critical step in the removal of polyubiquitinated proteins from macromolecular complexes and membranes for subsequent proteasomal degradation is the unfolding of an ubiquitin moiety by the cofactor Ufd1/Npl4 (UN) and its insertion into the ...
Cameron Williams, Ken C. Dong, Connor Arkinson, Andreas Martin   +3 more
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Site-directed fluorescence approaches to monitor the structural dynamics of proteins using intrinsic Trp and labeled with extrinsic fluorophores

STAR Protocols, 2022
Summary: Comprehensive understanding of a protein’s function depends on having reliable, sophisticated tools to study protein structural dynamics in physiologically-relevant conditions.
Rupasree Brahma, Anindita Das, H. Raghuraman   +2 more
doaj   +1 more source

CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

iScience, 2021
Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann   +8 more
doaj   +1 more source

Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration

STAR Protocols, 2021
Summary: We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration.
Yukino Matsui, Toshiki Akui, Nobuhide Doi, Kei Fujiwara   +3 more
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Biophysical and flavonoid-binding studies of the G protein ectodomain of group A human respiratory syncytial virus

Heliyon, 2019
The human Respiratory Syncytial Virus (hRSV) is the major causative agent of lower respiratory tract diseases in infants, young children and elderly. The membrane protein G is embedded in the viral lipid envelope and plays an adhesion function of the ...
Vitor Brassolatti Machado, Jéssica Maróstica de Sá, Ana Karla Miranda Prado, Karina Alves de Toledo, Luis Octávio Regasini, Fátima Pereira de Souza, Ícaro Putinhon Caruso, Marcelo Andres Fossey   +7 more
doaj   +1 more source

Watson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases

mBio, 2023
Replication-initiating HUH endonucleases (Reps) are sequence-specific nucleases that cleave and rejoin single-stranded DNA (ssDNA) during rolling-circle replication.
Adam T. Smiley, Kassidy J. Tompkins, Matthew R. Pawlak, August J. Krueger, Robert L. Evans, Ke Shi, Hideki Aihara, Wendy R. Gordon   +7 more
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Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators

Scientific Reports, 2023
We have used FRET-based biosensors in live cells, in a robust high-throughput screening (HTS) platform, to identify small-molecules that alter the structure and activity of the cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA2a). Our primary aim
Osha Roopnarine, Samantha L. Yuen, Andrew R. Thompson, Lauren N. Roelike, Robyn T. Rebbeck, Philip A. Bidwell, Courtney C. Aldrich, Razvan L. Cornea, David D. Thomas   +8 more
doaj   +1 more source