Results 31 to 40 of about 7,742 (179)

Molecular Full Adder Based on DNA Strand Displacement

IEEE Access, 2020
A new method, namely DNA strand displacement (DSD), has recently emerged in the field of biocomputing. DSD uses the toehold domain as a starting point to cover the strand in the migration domain.
Wei Xiao, Xinjian Zhang, Zheng Zhang, Congzhou Chen, Xiaolong Shi   +4 more
doaj   +1 more source

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

SeqBMC: Single‐cell data processing using iterative block matrix completion algorithm based on matrix factorisation

IET Systems Biology
With the development of high‐throughput sequencing technology, the analysis of single‐cell RNA sequencing data has become the focus of current research.
Gong Lejun, Yu Like, Wei Xinyi, Zhou Shehai, Xu Shuhua   +4 more
doaj   +1 more source

Open and remotely accessible Neuroplatform for research in wetware computing

Frontiers in Artificial Intelligence
Wetware computing and organoid intelligence is an emerging research field at the intersection of electrophysiology and artificial intelligence. The core concept involves using living neurons to perform computations, similar to how Artificial Neural ...
Fred D. Jordan, Martin Kutter, Jean-Marc Comby, Flora Brozzi, Ewelina Kurtys   +4 more
doaj   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

iGATTLDA: Integrative graph attention and transformer‐based model for predicting lncRNA‐Disease associations

IET Systems Biology
Long non‐coding RNAs (lncRNAs) have emerged as significant contributors to the regulation of various biological processes, and their dysregulation has been linked to a variety of human disorders.
Biffon Manyura Momanyi, Sebu Aboma Temesgen, Tian‐Yu Wang, Hui Gao, Ru Gao, Hua Tang, Li‐Xia Tang   +6 more
doaj   +1 more source

Large-scale prediction and analysis of protein sub-mitochondrial localization with DeepMito

BMC Bioinformatics, 2020
Background The prediction of protein subcellular localization is a key step of the big effort towards protein functional annotation. Many computational methods exist to identify high-level protein subcellular compartments such as nucleus, cytoplasm or ...
Castrense Savojardo, Pier Luigi Martelli, Giacomo Tartari, Rita Casadio   +3 more
doaj   +1 more source

Sclerostin regulates bone morphogenetic protein (BMP) bioavailability through interactions with BMP complexes and fibrillin microfibrils

The FEBS Journal, EarlyView.
Sclerostin (SOST) is a multifunctional extracellular modulator of BMP and Wnt signaling. SOST binds with high affinity to fibrillin‐1 and fibrillin‐2, localizing it to fibrillin microfibrils and regulating its extracellular bioavailability. Fibrillin‐bound SOST cannot engage BMP‐7 prodomain (PD) complexes, revealing a competitive mechanism that ...
Annkatrin Correns, Fady Marcous, Steffen Lütke, Matthias Mörgelin, Philipp Barnowski, Ariane G. Furlan, Nathalie H. Schröder, Bodo Levkau, Sarah Riek, Martin Mollenhauer, Manuel Koch, Gerhard Sengle   +11 more
wiley   +1 more source

Quantitative insights into the spatio‐temporal variation of Atlantic salmon (Salmo salar) biomass in a river catchment using eDNA metabarcoding

Journal of Fish Biology, EarlyView.
Abstract Effective species conservation and management requires comprehensive biomonitoring, enhanced by combining traditional and newer methodologies, such as environmental DNA (eDNA) analyses. A seasonal pulse of spawning adult Atlantic salmon (Salmo salar) was detected by normalised eDNA 12S reads from metabarcoding, which facilitated estimation of ...
William Bernard Perry, Nigel Milner, Ifan Bryn Jâms, Mark de Bruyn, Steve J. Ormerod, Kristy Deiner, Holly M. Bik, Gary Carvalho, John Colbourne, Bernard Jack Cosby, Isabelle Durance, Simon Creer   +11 more
wiley   +1 more source

Probing the molecular determinants of emission enhancement in RNA aptamer–metal complex systems

Photochemistry and Photobiology, EarlyView.
Light up aptamer: fluorophore pairs are widely used in sensing and imaging, with coordination complex fluorophores providing specific benefits due to their long‐lived emission. Here, we examined key structural and electronic features of these metal complexes to identify the requirements for productive engagement with an RNA aptamer.
Sarah M. Kriger, Joseph Spiconardi, Nathan Ennist, Edith C. Glazer   +3 more
wiley   +1 more source