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Autophagosome Biogenesis

open access: yesCells, 2023
Autophagy–the lysosomal degradation of cytoplasm–plays a central role in cellular homeostasis and protects cells from potentially harmful agents that may accumulate in the cytoplasm, including pathogens, protein aggregates, and dysfunctional organelles. This process is initiated by the formation of a phagophore membrane, which wraps around a portion of
Yan Zhen, Harald Stenmark
openaire   +4 more sources

Ancestral Stem Cell Reprogramming Genes Active in Hemichordate Regeneration

open access: yesFrontiers in Ecology and Evolution, 2022
Hemichordate enteropneust worms regenerate extensively in a manner that resembles the regeneration for which planaria and hydra are well known.
Tom Humphreys   +11 more
doaj   +1 more source

Golgi Biogenesis [PDF]

open access: yesCold Spring Harbor Perspectives in Biology, 2011
The Golgi is an essential membrane-bound organelle in the secretary pathway of eukaryotic cells. In mammalian cells, the Golgi stacks are integrated into a continuous perinuclear ribbon, which poses a challenge for the daughter cells to inherit this membrane organelle during cell division.
Yanzhuang, Wang, Joachim, Seemann
openaire   +2 more sources

Experimental and computational workflow for the analysis of tRNA pools from eukaryotic cells by mim-tRNAseq

open access: yesSTAR Protocols, 2022
Summary: Quantifying tRNAs is crucial for understanding how they regulate mRNA translation but is hampered by their extensive sequence similarity and premature termination of reverse transcription at multiple modified nucleotides.
Andrew Behrens, Danny D. Nedialkova
doaj   +1 more source

Phenotypic Characterization and Whole Genome Analysis of a Strong Biofilm-Forming Staphylococcus aureus Strain Associated With Subclinical Bovine Mastitis in Colombia

open access: yesFrontiers in Veterinary Science, 2020
Staphylococcus aureus represent a serious threat to public health due to food safety, antibiotic resistance, and the potential zoonotic transmission of strains between dairy cattle and humans. Biofilm formation by S.
Giovanny Torres   +5 more
doaj   +1 more source

Genetically-biased fertilization in APOBEC1 complementation factor (A1cf) mutant mice

open access: yesScientific Reports, 2022
Meiosis, recombination, and gametogenesis normally ensure that gametes combine randomly. But in exceptional cases, fertilization depends on the genetics of gametes from both females and males. A key question is whether their non-random union results from
Naoki Hirose   +8 more
doaj   +1 more source

Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions

open access: yesFrontiers in Plant Science, 2017
Lipid droplets (LDs) are cell compartments specialized for oil storage. Although their role and biogenesis are relatively well documented in seeds, little is known about their composition, structure and function in senescing leaves where they also ...
Lysiane Brocard   +18 more
doaj   +1 more source

Mutant-based model of two independent pathways for carotenoid-mediated chloroplast biogenesis in Arabidopsis embryos [PDF]

open access: yes, 2019
Chloroplasts are essential for autonomous plant growth, and their biogenesis is a complex process requiring both plastid and nuclear genome. One of the essential factors required for chloroplast biogenesis are carotenoids.
Colinas Martinez, Maite   +3 more
core   +1 more source

Biogenesis of Glyoxysomes [PDF]

open access: yes, 1980
Biosynthesis of isocitrate lyase, a tetrameric enzyme of the glyoxysomal matrix, was studied in Neurospora crassa, in which the formation of glyoxysomes was induced by a substitution of sucrose medium by acetate medium. * 1.
Neupert, Walter, Zimmermann, Richard
core   +1 more source

Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System

open access: yesMolecular Therapy: Nucleic Acids, 2013
Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system ...
Jesse B Owens   +5 more
doaj   +1 more source

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