Results 121 to 130 of about 322,466 (259)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Mitochondrial biogenesis in mammals: The role of endogenous nitric oxide

, 2003
Nitric oxide was found to trigger mitochondrial biogenesis in cells as diverse as brown adipocytes and 3T3-L1, U937, and HeLa cells. This effect of nitric oxide was dependent on guanosine 3′,5′-monophosphate (cGMP) and was mediated by the induction of ...
Francolini, Maura, Moncada, Salvador, R. Bracale, Carruba, Michele O., S. Moncada, M. Francolini, C. Sciorati, E. Nisoli, Valerio, Alessandra, C. Paolucci, Clementi, Emilio, Tonello, Cristina, Bracale, Renata, M.O. Carruba, C. Tonello, V. Cozzi, A. Valerio, Nisoli, Enzo, Paolucci, Clara, E. Clementi, Cozzi, Valeria, Sciorati, Clara   +21 more
core   +1 more source

USP30‐Mediated Deubiquitination of PEX5 Suppresses Pexophagy to Drive Tubular Injury in Diabetic Kidney Disease

Advanced Science, EarlyView.
USP30 accumulation in diabetic renal tubular epithelial cells deubiquitinates PEX5, suppresses pexophagy, and drives dysfunctional peroxisome accumulation and tubular injury. USP30 depletion restores PEX5 ubiquitination and pexophagy, improves peroxisomal function, and alleviates tubular damage, highlighting USP30 as a therapeutic target in diabetic ...
Jia Li, Chaoyang Hua, Guangpu Li, Furong Zhai, Jingjing Ren, Shaokang Pan, Qi Feng, Jianguo Wen, Dongwei Liu, Zhangsuo Liu, Jiayu Duan   +10 more
wiley   +1 more source

SPARC Drives Podocyte Mitochondrial Damage and Ferroptosis in Diabetic Kidney Disease Following Klotho Deficiency

Advanced Science, EarlyView.
Klotho deficiency promotes podocyte mitochondrial dysfunction and ferroptosis through activation of the PKCα/CUX1/SPARC/TGFβ‐RII axis. SPARC emerges as a key mediator linking Klotho loss to podocyte injury in DKD and other kidney injury models, suggesting broader implications for CKD progression.
Qing Yang, Huimin Jiang, Chenyang Xiong, Xindi Zhou, Qinqin Tan, Han Xiao, Lan Qiu, Chun Gan, Yaxi Chen, Haiping Yang, Wei Jiang, Qiu Li   +11 more
wiley   +1 more source

Co-regulation of pre-mRNA alternative splicing and microRNA biogenesis

, 2013
This study focuses on two ubiquitous post-transcriptional gene expression regulatory mechanisms: host gene pre-messenger RNA (pre-mRNA) alternative splicing and microRNA biogenesis.
Reich, Ashley, \u2713
core  

Biogenesis of inner membrane proteins in Escherichia coli

, 2012
The inner membrane proteome of the model organism Escherichia coli is composed of inner membrane proteins, lipoproteins and peripherally attached soluble proteins. Our knowledge of the biogenesis of inner membrane proteins is rapidly increasing.
Yu, Z., Wagner, Samuel, Wagner, S., Luirink, S.; id_orcid, Luirink, Joen, de Gier, Jan-Willem, Luirink, S., Yu, Zhong, de Gier, Jan-Willem,, de Gier, J.W.   +9 more
core   +1 more source

Photobiomodulation‑Engineered Extracellular Vesicles Enhance Neural Differentiation via UFL1‑Mediated UFMylation in Spinal Cord Injury

Advanced Science, EarlyView.
Photobiomodulated microglia release engineered extracellular vesicles that deliver UFL1 to the injured spinal cord. UFL1 competitively binds p53 with MDM2, inhibiting p53 ubiquitination and stabilizing p53 signaling. This dual mechanism simultaneously promotes anti‐inflammatory microglial polarization and directs neural stem cell differentiation toward
Yunxiao Fang, Zuomeng Wu, Ruocheng Guo, Cancan Wang, Yue Qin, Yixiang Dong, Aoyun Li, Ao Liu, Chongyu Jia, Tianyu Han, Peiwen Song, Lei Chen, Cailiang Shen   +12 more
wiley   +1 more source

Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis

, 2018
The paralogous Brr6 and Brl1 are conserved integral membrane proteins of the nuclear envelope (NE) with an unclear role in nuclear pore complex (NPC) biogenesis. Here, we analyzed double-degron mutants of Brr6/Brl1 to understand this function.
Timo Sachsenheimer, Annett Neuner, Britta Brügger, Christian Lüchtenborg, Wanlu Zhang, Elmar Schiebel, Diana Rüthnick   +6 more
core   +1 more source

A Micropeptide Component of the OGDH Complex Modulates ATP Production in the TCA Cycle

Advanced Science, EarlyView.
This study identified a mitochondrial micropeptide encoded by a non‐canonical open reading frame (alt‐ORF) within the CTSB gene. This micropeptide named APPM (ATP production‐promoting micropeptide) is a novel component of the OGDHc complex in the TCA cycle. APPM localizes to the inner mitochondrial membrane and directly binds to OGDH, the E1 subunit of
Xinqiang Yin, Weiyan Qi, Lifen Lin, Huilin Zheng, Guoxiang Zhao, Quan Chen, Guoqiang Shao, Yifan Wu, Jialiang Hu, Hanmei Xu   +9 more
wiley   +1 more source

What a unicellular parasite can tell us about mitochondrial biogenesis?

, 2013
Most of what we know about mitochondrial biogenesis stems from work in yeast and mammals, which are quite closely related. To understand the conserved features of mitochondria and the evolutionary forces that shaped it, it is important to study a more ...
Schneider, André
core