Tissue clearing and imaging methods for cardiovascular development
iScience, 2021 Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová +4 more
doaj
Robust model-based analysis of single-particle tracking experiments with Spot-On. [PDF]
, 2018 Single-particle tracking (SPT) has become an important method to bridge biochemistry and cell biology since it allows direct observation of protein binding and diffusion dynamics in live cells.
Darzacq, Xavier +5 more
core +2 more sources
Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
iScience, 2023 Summary: To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield ...
Christoph Drobek +5 more
doaj
An Optimal Likelihood Free Method for Biological Model Selection [PDF]
arXiv, 2022 Systems biology seeks to create math models of biological systems to reduce inherent biological complexity and provide predictions for applications such as therapeutic development. However, it remains a challenge to determine which math model is correct and how to arrive optimally at the answer.
arxiv
Prediction of Structures and Interactions from Genome Information [PDF]
In: H. Nakamura (ed.) Integrative Structural Biology with Hybrid
Methods, Advances in Experimental Medicine and Biology 1105, chap. 9.
Springer Nature Singapore Pte Ltd. (2018), 2017 Predicting three dimensional residue-residue contacts from evolutionary information in protein sequences was attempted already in the early 1990s. However, contact prediction accuracies of methods evaluated in CASP experiments before CASP11 remained quite low, typically with $<20$% true positives.
arxiv +1 more source
In-Cell Structural Biology by NMR: The Benefits of the Atomic Scale.
Chemical Reviews, 2022 In-cell structural biology aims at extracting structural information about proteins or nucleic acids in their native, cellular environment. This emerging field holds great promise and is already providing new facts and outlooks of interest at both ...
François-Xavier Theillet
semanticscholar +1 more source
Robust Detection of Hierarchical Communities from Escherichia coli Gene Expression Data [PDF]
, 2012 Determining the functional structure of biological networks is a central goal of systems biology. One approach is to analyze gene expression data to infer a network of gene interactions on the basis of their correlated responses to environmental and ...
A Beyer +64 more
core +6 more sources
Sensitive label-free imaging of brain samples using FxClear-based tissue clearing technique
iScience, 2021 Summary: Optical clearing has emerged as a powerful tool for volume imaging. Although volume imaging with immunostaining have been successful in many protocols, yet obtaining homogeneously stained thick samples remains challenging.
Boram Lee +12 more
doaj
Genetic tool development in marine protists: emerging model organisms for experimental cell biology
Nature Methods, 2019 Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life.
Drahomíra Faktorová +112 more
semanticscholar +1 more source
Time of day as a critical variable in biology
BMC Biology, 2022 Background Circadian rhythms are important for all aspects of biology; virtually every aspect of biological function varies according to time of day. Although this is well known, variation across the day is also often ignored in the design and reporting ...
Randy J. Nelson +9 more
semanticscholar +1 more source