Results 141 to 150 of about 7,863 (172)

Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions

2006
The bioluminescence resonance energy transfer (BRET) methodology allows for the study of protein-protein interactions as well as conformational changes within proteins or molecular complexes. BRET is a highly versatile technique that can be applied to in vitro studies using purified proteins, crude cell membranes, cell fractions obtained by ...
Tarik, Issad, Ralf, Jockers
openaire   +2 more sources

Biodegradable Polymer Nanoparticles for Photodynamic Therapy by Bioluminescence Resonance Energy Transfer

Biomacromolecules, 2017
Conventional photodynamic therapy is severely constrained by the limited light-penetration depth in tissue. Here, we show efficient photodynamic therapy (PDT) mediated by bioluminescence resonance energy transfer (BRET) that overcomes the light-penetration limitation.
Yingkun Yang, Weiying Hou, Siyang Liu, Kai Sun, Minyong Li, Changfeng Wu   +5 more
openaire   +2 more sources

Analysis of in vitro SUMOylation using bioluminescence resonance energy transfer (BRET)

Biochemical and Biophysical Research Communications, 2009
We demonstrated in vitro small ubiquitin-like modifier (SUMO)-mediated modification (SUMOylation) of RanGTPase activating protein-1 (RanGAP1) by using bioluminescence resonance energy transfer (BRET) for studying protein interactions. Renilla luciferase (Rluc) was fused to SUMO, and RanGAP1, the binding partner of SUMO, was fused to enhanced yellow ...
Kim, YP Kim, Young-Pil, Jin, Z Jin, Zongwen, Kim, EK Kim, Eun-Kyung, Park, SY Park, Sun-Young, Oh, YH Oh, Young-Hee, Kim, HS Kim, Hak-Sung   +5 more
openaire   +3 more sources

Demonstration of a Homogeneous Noncompetitive Immunoassay Based on Bioluminescence Resonance Energy Transfer

Analytical Biochemistry, 2001
We describe a noncompetitive homogeneous bioluminescent immunoassay based on the antigen-dependent reassociation of antibody variable domains (open sandwich bioluminescent immunoassay, OS-BLIA). The reassociation of two chimeric proteins, an antibody heavy-chain fragment (V(H))-Renilla luciferase (Rluc) and an antibody light-chain fragment (V(L ...
Ryoichi Arai, Kouhei Tsumoto, Hiroshi Ueda   +2 more
exaly   +3 more sources

Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays

2009
Recent advances in imaging assays based on bioluminescence resonance energy transfer (BRET) have made it possible to study protein/protein interactions in living cells under physiological conditions. Here we describe protocols for these assays including relevant positive and negative controls, and we also show how they can be combined with protein ...
Robitaille, Melanie, Heroux, Isabelle, Baragli, Alessandra, Hebert, Terence E.   +3 more
openaire   +5 more sources

Probing Bioluminescence Resonance Energy Transfer in Quantum Rod–Luciferase Nanoconjugates

ACS Nano, 2016
We describe the necessary design criteria to create highly efficient energy transfer conjugates containing luciferase enzymes derived from Photinus pyralis (Ppy) and semiconductor quantum rods (QRs) with rod-in-rod (r/r) microstructure. By fine-tuning the synthetic conditions, CdSe/CdS r/r-QRs were prepared with two different emission colors and three ...
Rabeka, Alam, Liliana M, Karam, Tennyson L, Doane, Kaitlin, Coopersmith, Danielle M, Fontaine, Bruce R, Branchini, Mathew M, Maye   +6 more
openaire   +2 more sources

Engineering Aspects of Bioluminescence Resonance Energy Transfer Systems

2013
Development of optical probes for sensing biological functions in vivo is in high demand in modern biology. With several inherent advantages, the bioluminescence resonance energy transfer (BRET)-based sensors are rapidly expanding and showing great utilities in the study of protein–protein interactions (PPIs), protein dimerization, signal transduction,
Abhijit De, Rohit Arora, Akshi Jasani
openaire   +1 more source

Measuring IL-1β Processing by Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor

2016
IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and
Vincent, Compan, Pablo, Pelegrín
openaire   +3 more sources

Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer

2016
Chemokine receptors recruit the multifunctional scaffolding protein beta arrestin in response to binding of their chemokine ligands. Given that arrestin recruitment represents a signaling axis that is in part independent from G-protein signaling, it has become a hallmark of G protein-coupled receptor functional selectivity. Therefore, quantification of
J, Bonneterre, N, Montpas, C, Boularan, C, Galés, N, Heveker   +4 more
openaire   +2 more sources

Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling

2015
Bioluminescence resonance energy transfer (BRET) is a well-established technique for investigating G protein-coupled receptor (GPCR) pharmacology. BRET enables the monitoring of molecular proximity through the use of heterologously expressed proteins of interest and/or fluorophore-labeled ligands.
Elizabeth K M, Johnstone, Kevin D G, Pfleger   +1 more
openaire   +2 more sources