Results 151 to 160 of about 7,863 (172)

Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly

2008
Bioluminescence Resonance Energy Transfer (BRET) measures protein interactions within 10 nm of each other. Aside from its ability to probe for interactions at high resolution, this technique operates in live, intact cells, and offers a high throughput method of detection. Thus far, BRET has been widely used in measuring G protein receptor dimerization.
Gina M, Whitaker, Eric A, Accili
openaire   +2 more sources

Bioanalytical Systems Based on Bioluminescence Resonance Energy Transfer Using Firefly Luciferase

Combinatorial Chemistry & High Throughput Screening, 2015
Bioanalytical systems based on the Bioluminescence Resonance Energy Transfer (BRET) are widely used in fundamental biochemical studies, as well as for screening and analysis of biologically active compounds. The Renilla luciferase is the most often used energy donor in this system despite the fact that it has low bioluminescence quantum yield and ...
Darya V, Smirnova, Natalia N, Ugarova
openaire   +2 more sources

Monitoring the Activation State of the Insulin Receptor Using Bioluminescence Resonance Energy Transfer

Molecular Pharmacology, 2001
We have developed a procedure based on bioluminescence resonance energy transfer (BRET) to monitor the activation state of the insulin receptor in vitro. Human insulin receptor cDNA was fused to either Renilla luciferase (Rluc) or enhanced yellow fluorescent protein (EYFP) coding sequences. Fusion insulin receptors were partially purified by wheat-germ
N, Boute, K, Pernet, T, Issad
openaire   +2 more sources

Dimerization of the melanocortin 4 receptor: A study using bioluminescence resonance energy transfer

Peptides, 2006
The melanocortin 4 receptor is important in the regulation of satiety. In this study we have investigated the propensity of the MC4 receptor to homodimerize. MC4 receptors with either a modified green fluorescent protein (GFP(2)) or Renilla luciferase (RLuc) at their C-terminus were constructed.
Sarah A, Nickolls, Rich A, Maki
openaire   +2 more sources

Glycoprotein-glycoprotein Receptor Binding Detection Using Bioluminescence Resonance Energy Transfer

Endocrinology
Abstract The glycoprotein receptors, members of the large G protein-coupled receptor family, are characterized by a large extracellular domains responsible for binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays, most often using radiolabeling but also by thermal shift
Kamila Adamczuk, Thu Ha Ngo, Jakub Czapiński, Adolfo Rivero-Müller   +3 more
openaire   +3 more sources

Analysis of Protein–Protein Interactions Using Bioluminescence Resonance Energy Transfer

2009
Knowledge of how and when proteins interact in living cells is fundamental to our understanding of cellular biology, and bioluminescence resonance energy transfer (BRET) provides an increasingly popular mechanism for studying these interactions in real time.
openaire   +2 more sources

Detection of ERα-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer

2009
Bioluminescent Resonance Energy Transfer is a naturally occurring phenomenon that can be exploited to explore protein-protein interactions in real-time in intact cells and cellular extracts. It detects energy transferred between a bioluminescent donor enzyme (Renilla luciferase) fusion protein and a fluorescent (GFP(2), a mutant of Green Fluorescent ...
Tamika T, Duplessis, Kristen L, Koterba, Brian G, Rowan   +2 more
openaire   +2 more sources

Firefly Luciferase-Based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assays

2016
We describe here the preparation of ratiometric luminescent probes that contain two well-separated emission peaks produced by a sequential bioluminescence resonance energy transfer (BRET)-fluorescence resonance energy transfer (FRET) process. The probes are single soluble fusion proteins consisting of a thermostable firefly luciferase variant that ...
openaire   +2 more sources

Ratiometric Detection of Zn2+ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors

Chemistry, 2023
Jing Luen Wai, Ying Lu
exaly  

A red-shifted Bioluminescence Resonance Energy Transfer (BRET) biosensing system for rapid measurement of plasmin activity in human plasma

Analytica Chimica Acta, 2020
Felix Weihs, Helen Dacres, Alex PEH
exaly