Results 151 to 160 of about 6,388 (174)

BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) ASSAY OF THE RAS/RAF INTERACTION

2017
The ERK/MAPK cascade plays a central role in the regulation of cellular growth, proliferation, and differentiation, and is dependent upon the binding of the Raf kinases to activated, GTP-bound Ras. The three Ras family members, HRas, KRas, and NRas, share 85% sequence identity, and yet show unique distribution within different cancer subtypes ...
openaire   +1 more source

Firefly Luciferase-Based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assays

2016
We describe here the preparation of ratiometric luminescent probes that contain two well-separated emission peaks produced by a sequential bioluminescence resonance energy transfer (BRET)-fluorescence resonance energy transfer (FRET) process. The probes are single soluble fusion proteins consisting of a thermostable firefly luciferase variant that ...
openaire   +2 more sources

A suite of tools and application notes for in vivo protein interaction assays using bioluminescence resonance energy transfer (BRET).

The Plant Journal, 2006
C. Subramanian, J. Woo, Xue Cai, Xiaodong Xu, Stein Servick, C. Johnson, A. Nebenführ, A. V. von Arnim   +7 more
semanticscholar   +1 more source

DEVELOPMENT OF A SENSITIVE INSTRUMENT FOR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) APPLICATIONS

Bioluminescence and Chemiluminescence, 2002
F BERTHOLD, C H JOHNSON, A KANAUCHI, A HEDING, M PEUKERT, M HENNECKE, B HUTTER   +6 more
openaire   +1 more source

Bioluminescence Resonance Energy Transfer (BRET)‐coupled Annexin V‐functionalized Quantum Dots for Near‐Infrared Optical Detection of Apoptotic Cells

ChemBioChem, 2018
Setsuko Tsuboi, T. Jin
semanticscholar   +1 more source

Novel, isotype-specific sensors for protein kinase A subunit interaction based on bioluminescence resonance energy transfer (BRET).

Cellular Signalling, 2006
A. Prinz, M. Diskar, Andrea Erlbruch, F. Herberg   +3 more
semanticscholar   +1 more source

Probing intermolecular protein-protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET).

European Journal of Biochemistry, 2002
A. Jensen, J. L. Hansen, S. Sheikh, H. Bräuner‐Osborne   +3 more
semanticscholar   +1 more source

New Technologies: Bioluminescence Resonance Energy Transfer (BRET) for the Detection of Real Time Interactions Involving G-Protein Coupled Receptors

Pituitary, 2004
K. Pfleger, K. Eidne
semanticscholar   +1 more source

Using Bioluminescence Resonance Energy Transfer (BRET) to Characterize Agonist‐Induced Arrestin Recruitment to Modified and Unmodified G Protein‐Coupled Receptors

Current Protocols in Pharmacology, 2015
Jonathan Javitch, Nevin Lambert, Vsevolod Gurevich   +2 more
exaly  

Bioluminescence resonance energy transfer (BRET) systems and methods of use thereof

2007
GAMBHIR SANJIV S, DE ABHIJIT
openaire   +3 more sources