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Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells
2019Detection of apoptotic cells is crucial for understanding the mechanism of diseases and for therapy development. So far, visible-emitting fluorescent probes such as FITC-labeled Annexin V has been widely used for the detection of apoptotic cells. However, such probes cannot be applied to noninvasive imaging in the near-infrared (NIR) region.
Setsuko, Tsuboi, Takashi, Jin
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Biosensors and Bioelectronics, 2011
Here we report the design of a bioluminescence resonance energy transfer (BRET)-based sensing system that could detect nucleic acid target in 5 min with high sensitivity and selectivity. The sensing system is based on adjacent binding of oligonucleotide probes labeled with Renilla luciferase (Rluc) and quantum dot (Qd) on the nucleic acid target.
Manoj Kumar +2 more
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Here we report the design of a bioluminescence resonance energy transfer (BRET)-based sensing system that could detect nucleic acid target in 5 min with high sensitivity and selectivity. The sensing system is based on adjacent binding of oligonucleotide probes labeled with Renilla luciferase (Rluc) and quantum dot (Qd) on the nucleic acid target.
Manoj Kumar +2 more
exaly +3 more sources
2023
Abstract The glycoprotein receptors, members of the large G protein-coupled receptors (GPCRs) family, are characterized by a large extracellular domains responsible of binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays most often using radiolabeling ...
Kamila Adamczuk, Adolfo Rivero-Müller
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Abstract The glycoprotein receptors, members of the large G protein-coupled receptors (GPCRs) family, are characterized by a large extracellular domains responsible of binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays most often using radiolabeling ...
Kamila Adamczuk, Adolfo Rivero-Müller
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Methods in Molecular Biology, 2016
Bioluminescence resonance energy transfer (BRET) is a prominent biophysical technology for monitoring molecular interactions, and has been widely used to study protein-protein interactions (PPI) in live cells. This technology requires proteins of interest to be associated with an energy donor (i.e., luciferase) and an acceptor (e.g., fluorescent ...
Haian Fu, Fu Haian
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Bioluminescence resonance energy transfer (BRET) is a prominent biophysical technology for monitoring molecular interactions, and has been widely used to study protein-protein interactions (PPI) in live cells. This technology requires proteins of interest to be associated with an energy donor (i.e., luciferase) and an acceptor (e.g., fluorescent ...
Haian Fu, Fu Haian
exaly +3 more sources
Biosensors and Bioelectronics, 2014
We have previously shown that a genetically encoded bioluminescent resonance energy transfer (BRET) biosensor, comprising maltose binding protein (MBP) flanked by a green fluorescent protein (GFP(2)) at the N-terminus and a variant of Renilla luciferase (RLuc2) at the C-terminus, has superior sensitivity and limits of detection for maltose, compared ...
Murat Gel, Yonggang Zhu, Helen Dacres
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We have previously shown that a genetically encoded bioluminescent resonance energy transfer (BRET) biosensor, comprising maltose binding protein (MBP) flanked by a green fluorescent protein (GFP(2)) at the N-terminus and a variant of Renilla luciferase (RLuc2) at the C-terminus, has superior sensitivity and limits of detection for maltose, compared ...
Murat Gel, Yonggang Zhu, Helen Dacres
exaly +3 more sources
Current Protocols in Neuroscience, 2006
AbstractBioluminescence resonance energy transfer (BRET) allows monitoring of protein‐protein interactions in real time in living cells. One candidate interacting protein is fused to a luminescent energy donor, such asRenillaluciferase, and the other to a fluorescent energy acceptor, such the green fluorescent protein (GFP), and the two are then ...
Fadi F, Hamdan +3 more
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AbstractBioluminescence resonance energy transfer (BRET) allows monitoring of protein‐protein interactions in real time in living cells. One candidate interacting protein is fused to a luminescent energy donor, such asRenillaluciferase, and the other to a fluorescent energy acceptor, such the green fluorescent protein (GFP), and the two are then ...
Fadi F, Hamdan +3 more
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SPIE Proceedings, 2004
Estrogen receptor (ER) is a ligand-activated transcriptional factor, able to dimerize after activation and to bind specific DNA sequences (estrogen response elements), thus activating gene target transcription. Since ER homo- and hetero-dimerization (giving a-a and a-b isoforms) is a fundamental step for receptor activation, we developed an assay for ...
Elisa Michelini +4 more
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Estrogen receptor (ER) is a ligand-activated transcriptional factor, able to dimerize after activation and to bind specific DNA sequences (estrogen response elements), thus activating gene target transcription. Since ER homo- and hetero-dimerization (giving a-a and a-b isoforms) is a fundamental step for receptor activation, we developed an assay for ...
Elisa Michelini +4 more
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BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) ASSAY OF THE RAS/RAF INTERACTION
2017The ERK/MAPK cascade plays a central role in the regulation of cellular growth, proliferation, and differentiation, and is dependent upon the binding of the Raf kinases to activated, GTP-bound Ras. The three Ras family members, HRas, KRas, and NRas, share 85% sequence identity, and yet show unique distribution within different cancer subtypes ...
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2016
The bioluminescent resonance energy transfer (BRET) assay has been extensively used in cell-based and in vivo imaging systems for detecting protein-protein interactions in the native environment of living cells. These protein-protein interactions are essential for the functional response of many signaling pathways to environmental chemicals.
Carlos, Coriano, Emily, Powell, Wei, Xu
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The bioluminescent resonance energy transfer (BRET) assay has been extensively used in cell-based and in vivo imaging systems for detecting protein-protein interactions in the native environment of living cells. These protein-protein interactions are essential for the functional response of many signaling pathways to environmental chemicals.
Carlos, Coriano, Emily, Powell, Wei, Xu
openaire +2 more sources