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2023
Abstract The glycoprotein receptors, members of the large G protein-coupled receptors (GPCRs) family, are characterized by a large extracellular domains responsible of binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays most often using radiolabeling ...
Kamila Adamczuk, Adolfo Rivero-Müller
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Abstract The glycoprotein receptors, members of the large G protein-coupled receptors (GPCRs) family, are characterized by a large extracellular domains responsible of binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays most often using radiolabeling ...
Kamila Adamczuk, Adolfo Rivero-Müller
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Current Protocols in Neuroscience, 2006
AbstractBioluminescence resonance energy transfer (BRET) allows monitoring of protein‐protein interactions in real time in living cells. One candidate interacting protein is fused to a luminescent energy donor, such asRenillaluciferase, and the other to a fluorescent energy acceptor, such the green fluorescent protein (GFP), and the two are then ...
Fadi F, Hamdan +3 more
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AbstractBioluminescence resonance energy transfer (BRET) allows monitoring of protein‐protein interactions in real time in living cells. One candidate interacting protein is fused to a luminescent energy donor, such asRenillaluciferase, and the other to a fluorescent energy acceptor, such the green fluorescent protein (GFP), and the two are then ...
Fadi F, Hamdan +3 more
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BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) ASSAY OF THE RAS/RAF INTERACTION
2017The ERK/MAPK cascade plays a central role in the regulation of cellular growth, proliferation, and differentiation, and is dependent upon the binding of the Raf kinases to activated, GTP-bound Ras. The three Ras family members, HRas, KRas, and NRas, share 85% sequence identity, and yet show unique distribution within different cancer subtypes ...
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SPIE Proceedings, 2004
Estrogen receptor (ER) is a ligand-activated transcriptional factor, able to dimerize after activation and to bind specific DNA sequences (estrogen response elements), thus activating gene target transcription. Since ER homo- and hetero-dimerization (giving a-a and a-b isoforms) is a fundamental step for receptor activation, we developed an assay for ...
Elisa Michelini +4 more
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Estrogen receptor (ER) is a ligand-activated transcriptional factor, able to dimerize after activation and to bind specific DNA sequences (estrogen response elements), thus activating gene target transcription. Since ER homo- and hetero-dimerization (giving a-a and a-b isoforms) is a fundamental step for receptor activation, we developed an assay for ...
Elisa Michelini +4 more
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2016
The bioluminescent resonance energy transfer (BRET) assay has been extensively used in cell-based and in vivo imaging systems for detecting protein-protein interactions in the native environment of living cells. These protein-protein interactions are essential for the functional response of many signaling pathways to environmental chemicals.
Carlos, Coriano, Emily, Powell, Wei, Xu
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The bioluminescent resonance energy transfer (BRET) assay has been extensively used in cell-based and in vivo imaging systems for detecting protein-protein interactions in the native environment of living cells. These protein-protein interactions are essential for the functional response of many signaling pathways to environmental chemicals.
Carlos, Coriano, Emily, Powell, Wei, Xu
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Bioluminescence resonance energy transfer (BRET) systems and methods of use thereof
2007GAMBHIR SANJIV S, DE ABHIJIT
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A BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) SYSTEM AND ITS USE
2000JOLY ERIK +2 more
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