The role of SUMOylation in biomolecular condensate dynamics and protein localization. [PDF]
Cell InsightAs a type of protein post-translational modification, SUMOylation is the process that attaches a small ubiquitin-like modifier (SUMO) to lysine residues of protein substrates. Not only do SUMO and ubiquitin exhibit structure similarity, but the enzymatic
Gutierrez-Morton E, Wang Y.
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Soft MatterBiomolecular condensates, including those formed by prion-like low complexity domains (LCDs) of proteins, are maintained by networks of molecular interactions.
Tan D, Aierken D, Garcia PL, Joseph JA.
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Converging frontiers in biomolecular condensate and synthetic cell research [PDF]
NPJ Biomed InnovBiomolecular condensate and artificial cell research, though distinct in origin, share a focus on studying and mimicking (sub)cellular compartments. Identifying converging frontiers offers opportunities to advance both fields.
Verwiel M, Erkamp N, Hest J.
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The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops. [PDF]
Nucleic Acids Res, 2021 The nucleolus is the site of ribosome biosynthesis encompassing the ribosomal DNA (rDNA) locus in a phase separated state within the nucleus. In budding yeast, we find the rDNA locus and Cdc14, a protein phosphatase that co-localizes with the rDNA ...
Lawrimore J +7 more
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Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition. [PDF]
BiomacromoleculesAccurate determination of biomolecular condensate volume reveals that destabilization of condensates can lead to either swelling or shrinking of condensates, giving fundamental insights into the regulation of the volume of cellular condensates ...
Smokers IBA, Spruijt E.
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ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells. [PDF]
J Neurochem, 2021 Mutations in Ubiquilin-2 (UBQLN2), a ubiquitin-binding shuttle protein involved in several protein quality control processes, can lead to amyotrophic lateral sclerosis (ALS).
Riley JF +4 more
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Regulation of biomolecular condensate dynamics by signaling. [PDF]
Curr Opin Cell Biol, 2021 Biomolecular condensates are mesoscopic biomolecular assemblies devoid of long range order that contribute to important cellular functions. They form reversibly, are stabilized by numerous but relatively weak intermolecular interactions, and their ...
Garcia-Cabau C, Salvatella X.
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Higher-order organization of biomolecular condensates [PDF]
Open Biology, 2021 A guiding principle of biology is that biochemical reactions must be organized in space and time. One way this spatio-temporal organization is achieved is through liquid–liquid phase separation (LLPS), which generates biomolecular condensates.
Charlotte M. Fare +3 more
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Multivalent tumor suppressor adenomatous polyposis coli promotes Axin biomolecular condensate formation and efficient β-catenin degradation. [PDF]
Sci Rep, 2020 The tumor suppressor adenomatous polyposis coli (APC) is frequently mutated in colorectal cancers. APC and Axin are core components of a destruction complex that scaffolds GSK3β and CK1 to earmark β-catenin for proteosomal degradation.
Li TM +5 more
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Topography-driven movement of biomolecular condensates. [PDF]
PLoS ONEBiomolecular condensates are assemblies of proteins or nucleic acids that exhibit liquid-like properties and organize intracellular biochemical reactions within many cells.
Matthias Pöllmann, Katja Zieske
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