Results 161 to 170 of about 96,256 (296)

Cardiogenesis of embryonic stem cells with liquid marble micro-bioreactor

, 2015
A liquid marble micro-bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2-ESC) suspension onto a polytetrafluoroethylene (PTFE) particle bed. The Oct4B2-ESC aggregates to form embryoid bodies (EBs) with relatively uniform
Peggy Chan (4971193), Kanika Jain (2884439), Fatemeh Sarvi (15269417), Tina Arbatan (20025789), Paul Verma (639226), Kerry Hourigan (420265), Mark Thompson (533940), Wei Shen (149127)   +7 more
core  

Assessing the performance of aged field-scale woodchip bioreactors in Iowa. [PDF]

J Environ Qual
Buseman A, Soupir M, Christianson LE.
europepmc   +1 more source

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing

Biotechnology Progress, EarlyView.
Abstract Maintaining consistent quality in the manufacturing of biotherapeutic proteins in mammalian cell culture is challenging, with unplanned deviations causing inconsistencies and potential batch failure. Current methods for monitoring and controlling critical process parameters (CPPs) rely on slow, labor‐intensive offline analyses.
Matthew Banner, Daniel Ray, Irfan Ahanger, Fabio Zurlo, Noah Hitchcock, Rajesh Mistry, Kasia Kozakowska‐McDonnell, Matthew Cheeks, Richard Turner, Jonathan Welsh, Suzanne S. Farid, Michael Thomas, Stephen Goldrick   +12 more
wiley   +1 more source

Improving the reproducibility of mixing-time experiments in stirred single-use bioreactors. [PDF]

Appl Microbiol Biotechnol
Barth I, Holbeck C, Capitain C, Schirmeister T, Kampeis P.   +4 more
europepmc   +1 more source

In‐line Raman spectroscopy real‐time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control

Biotechnology Progress, EarlyView.
Abstract Traditional off‐line analysis methods to quantify residual metabolite concentrations in a drug substance fermentation process consume valuable time, require costly resources, and demand potentially hazardous manual operations when working with pathogenic biological organisms.
Griffin P. Thomas, Brian C. Marks, Barbara B. Alvarado‐Hernandez, Matthew Woodling, James O'Connor, Roberto Ortiz, John Higgins, Jon Shanter   +7 more
wiley   +1 more source

Bioreactor Technology for Medicinal Plant In Vitro Cultures: Systems, Applications, and Future Perspectives. [PDF]

Biology (Basel)
Zhang S, Ma M, Liu Y, Jiang H, Gao J, Yang Q, Wei K.   +6 more
europepmc   +1 more source

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo‐Voigt hard modeling during anion‐exchange chromatography

Biotechnology Progress, EarlyView.
Abstract In biopharmaceutical manufacturing, protein aggregation is a critical quality attribute, necessitating rapid and reliable analytical strategies during downstream processes like anion‐exchange chromatography (AEX). While Raman spectroscopy enables continuous monitoring of protein secondary structure, standard data‐driven regression models ...
Jakob Heyer‐Müller, Robin Schiemer, Lars Robbel, Michael Schmitt, Jürgen Hubbuch   +4 more
wiley   +1 more source

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials. [PDF]

Appl Microbiol Biotechnol
Barth I, Fitschen J, Sieblist C, Husemann U, Kues D, Neubrand C, Bouvier C, Schirmer C, Tscheschke B, Vassilev I, Kampeis P.   +10 more
europepmc   +1 more source

Scalable catalyst production process for oleate hydratase whole‐cell biocatalysis

Biotechnology Progress, EarlyView.
This study establishes a scalable lactose‐induced fed‐batch fermentation for an Escherichia coli whole‐cell biocatalyst containing an oleate hydratase. Operation at a higher growth rate markedly increased biomass yields while maintaining catalyst activity and was demonstrated at 150 L scale.
Rebekka Horstmann, Mario Beckers, Jörn Viell, Fabian Allan Watt, Valentin Steier, Marcel Mann, Jørgen B. Magnus   +6 more
wiley   +1 more source

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP)

Biotechnology Progress, EarlyView.
Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Aylin Mohammadzadehmarandi, Nate Heller, Mohammadreza Ahmadimashhadi, Jon Petrone, Nicholas Marchand, Siavash Darvishmanesh, Ka Zhang, Stefano Menegatti, Andrew L. Zydney   +8 more
wiley   +1 more source