Results 161 to 170 of about 81,744 (305)

Automated and scalable expansion of human liver organoids for translational applications. [PDF]

J Transl Med
Ten Dam MJM, Jno Baptiste-Sam JS, Schwanen RS, van Uden L, Verstegen MMA, van der Laan LJW, Fuchs SA, Das R, Spee B.   +8 more
europepmc   +1 more source

Asymmetric Dialysis: Truly Unified and Simultaneous Single‐Pass Concentration and Buffer Exchange

Biotechnology and Bioengineering, EarlyView.
Asymmetric dialysis, a novel one‐step continuous process combining ultrafiltration and diafiltration, achieves high product concentrations (up to 200 g/L) with low pressures and reduces buffer consumption by 75% compared to conventional methods. This sustainable, low‐cost technology demonstrates significant potential for integration into continuous ...
Ujwal Patil, Michelle Chen, Irina Ramos, Jon Coffman   +3 more
wiley   +1 more source

Fixed-Bed Bioreactor Culture Enhances Yield and Reparative Properties of hTERT Mesenchymal Stem Cell Extracellular Vesicles. [PDF]

Cells
Cuba Z, Godinho L, Choudhury S, Patil K, Williams A, Zhou W, Howard M, Aryal SP, Clayton KA, Routenberg DA, Liotta LA, Couch H, Kashanchi F, Branscome H.   +13 more
europepmc   +1 more source

A Modular Mechanistic In Silico Model for In Vitro Transcription Process Yield and Product Quality Prediction

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In vitro transcription (IVT) plays a critical role in the manufacture of mRNA vaccines and therapeutics. Optimizing mRNA yield and ensuring product quality, such as capping efficiency and integrity, are essential but mechanistically complex. This study presents a modular mechanistic model of the IVT process to advance scientific understanding ...
Keqi Wang, Keilung Choy, Eli Reiser, Jinxiang Pei, Hua Zheng, Aparajita Dasgupta, Fuqiang Cheng, Guogang Dong, Bhanu Chandra Mulukutla, Joshua Mannheimer, Carolyn Huang, Hooman Farsani, Wei Xie   +12 more
wiley   +1 more source

Nicotinamide Reverses the Warburg Effect in CHO Cell Culture

Biotechnology and Bioengineering, EarlyView.
Abstract The Warburg effect, the preferential conversion of glucose‐derived pyruvat to lactate despite available oxygen, is a key feature of Chinese hamster ovary (CHO) cell culture. Lactate accumulation in recombinant protein‐producing cell culture is an inefficient usage of glucose, as well as being deleterious to cells.
James Morrissey, Ayca Cankorur‐Cetinkaya, Luigi Grassi, Annie J. Harwood‐Stamper, Jonathan Welsh, Ryte Poskute, Kasia Kozakowska‐McDonnell, Cleo Kontoravdi   +7 more
wiley   +1 more source

Stirred tank bioreactor process for chikungunya vaccine candidate VEEV-ΔC-CHIKV. [PDF]

PLoS One
Chang T, Deng C, Xie L, Zheng Y, Zhang Y, Liu J, Fu Z, Zhang B, An Q, Tian D.   +9 more
europepmc   +1 more source

Detergent Virus Inactivation in Chromatography Systems

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Detergent treatment is a widely utilized virus‐inactivation step in therapeutic protein manufacturing to safeguard products. Traditionally, this operation is performed in an incubation vessel in batch mode. In this investigation, a methodology was developed to enable virus inactivation via a post‐load, detergent‐containing wash within a bind ...
Kang Cai, Etienne Utiger, Chris Afdahl, Rayan Zamat, Jennifer Anderson, Yeldys De Armas, Gisela Ferreira   +6 more
wiley   +1 more source

Production of an enzymatic cocktail by Aspergillus japonicus in a stirred-tank bioreactor for application in animal feed. [PDF]

World J Microbiol Biotechnol
de Oliveira Simas AL, Almeida AP, Glienke NN, de Melo Santana Q, Silva Galeano RM, Kiefer C, de Souza Nascimento KMR, de Moraes Polizeli MLT, Masui DC, Zanoelo FF, Giannesi GC.   +10 more
europepmc   +1 more source

Dynamic, Unconstrained Optimization of Secreted Enzyme Production in Fed‐Batch Fermentation Using Reinforcement Learning

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Reinforcement learning (RL) has been used to control a wide range of dynamic processes, especially ones that are too complex to model well or have stochastic environmental perturbations. Fed‐batch fermentations are subject to changes in starting cell growth rates and process variations that can affect cell growth and secreted target production.
Sai Harish Uthravalli, Sakib Ferdous, J. Michael Hess, Nigel F. Reuel   +3 more
wiley   +1 more source

Robust ammonia oxidation by "<i>Candidatus</i> Nitrosacidococcus tergens" across a broad pH range. [PDF]

mBio
Peterse IF, Frey C, Egas RA, Nuijten GHL, Veraart AJ, Lücker S.   +5 more
europepmc   +1 more source