Results 201 to 210 of about 81,744 (305)

Rapid development of a transferable Raman model using high‐throughput cell culture for monitoring monoclonal antibody titer

Biotechnology Progress, EarlyView.
Abstract Monoclonal antibody (mAb) titer monitoring is a key capability during process development and optimization, enabling timely decision making and increasing the speed of development. Raman spectroscopy is a prominent process analytical technology (PAT), but resource‐efficient calibration strategies for the development of transferable models are ...
Alexandra Umprecht, Nicholas Uth, Haenah Kim, Oliver Spadiut, Yang Yang   +4 more
wiley   +1 more source

Impact of process parameters on IgG glycosylation in CHO systems: a comprehensive quantitative analysis. [PDF]

MAbs
Bravo-Venegas J, Rodriguez-Siza J, Vergara M, Torres M, Dickson A, Toledo JR, Molina MC, Hermoso MA, Berríos J, Altamirano C.   +9 more
europepmc   +1 more source

Multivariate phase‐dependent optimization of bioprocesses boosts performance and quality—Why timing (of exposure) matters

Biotechnology Progress, EarlyView.
Abstract Applying a single parameter set to describe complex mammalian kinetics often is too simplistic, as it fails to capture sensitive cell‐to‐environment interactions that may be exploited to optimize production performance. To resolve this time dependency, intra‐experimental parameter shifts as part of design of dynamic experiments (DoDE) can be ...
Samuel Kienzle, Lisa Junghans, Anja Wittmann, Stefan Wieschalka, Ralf Takors, Nicole Erika Radde, Beate Presser, Verena Nold   +7 more
wiley   +1 more source

Editorial: Advanced bioprocessing strategies for tissue engineering and biomimetic modelling applications. [PDF]

Front Bioeng Biotechnol
Silva JC, Rodrigues CAV, Velliou E, Massai D.   +3 more
europepmc   +1 more source

On‐deck pH measurement with integrated feedback control on a micro‐scale platform for monoclonal antibody low pH viral inactivation

Biotechnology Progress, EarlyView.
Integrating an automated, on‐deck pH measurement method with feedback control enables precise, real‐time pH adjustment during low pH viral inactivation on a micro‐scale platform. Abstract The increasing demand for efficient monoclonal antibody manufacturing has accelerated the adoption of high throughput process development (HTPD) platforms, which ...
Paras Sharma, Nikolas von den Eichen, Sabine Schweisgut, Lars Robbel, Michael Schmitt, Daniel G. Bracewell   +5 more
wiley   +1 more source

Anaerobic Bioconversion of Mixed Fruit Waste into Organic Acids and a Multifunctional Enzymatic Bioproduct in a Stirred-Tank Bioreactor Using <i>Wickerhamomyces</i> sp. UFFS-CE-3.1.2. [PDF]

Microorganisms
Longo VD, Silveira NMFP, Amorim MP, Silva EFD, Baldasso IS, Fornari AC, Nazari MT, Alves SL, Treichel H.   +8 more
europepmc   +1 more source

Machine learning‐assisted clone selection for intensified cell culture processes

Biotechnology Progress, EarlyView.
Abstract Intensified fed‐batch processes are becoming increasingly prevalent among biomanufacturers due to their superior space–time yields relative to traditional, non‐intensified fed‐batch processes. However, the shift towards intensified manufacturing has unexpectedly made optimal clone selection more challenging.
Nicolas Wolnick, John Schmitt, Christian Siltanen, Julia Armstrong, Shannon Hendrick, Ariel Atchley, Brandon Downey   +6 more
wiley   +1 more source

Correction: Analysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor. [PDF]

Microbiome
Granato A, Renwick S, Yau C, Kong T, Daigneault MC, Knip M, DIABIMMUNE Study Group, Allen-Vercoe E, Danska JS.   +8 more
europepmc   +1 more source

β‐1,4‐galactosyltransferase 1 inhibitors modulate cellular N‐glycan profiles

Biotechnology Progress, EarlyView.
Graphical representation of the study workflow used to investigate the effects of known in vitro galactosyltransferase inhibitors on cell culture performance and recombinant IgG galactosylation. Abstract N‐glycosylation is a key post‐translational modification of proteins influencing their physicochemical properties as well as biological activity. This
Jaka Kranjc, Stane Pajk, Marko Anderluh, Anja Pišlar, Matjaž Brinc   +4 more
wiley   +1 more source

Development of a pandemic H5N1 influenza vaccine using MDCK suspension cells: A scalable and efficient production platform. [PDF]

Hum Vaccin Immunother
Zhang S, Wang M, Jin D, Zhang J, Wang J, Ma Z, Yang J.   +6 more
europepmc   +1 more source