Results 171 to 180 of about 111,398 (363)

Scaling‐down biopharmaceutical production processes via a single multi‐compartment bioreactor (SMCB)

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Biopharmaceutical production processes often use mammalian cells in bioreactors larger than 10,000 L, where gradients of shear stress, substrate, dissolved oxygen and carbon dioxide, and pH are likely to occur. As former tissue cells, producer cell lines such as Chinese hamster ovary (CHO) cells sensitively respond to these mixing ...
Lena Gaugler, Yannic Mast, Jürgen Fitschen, Sebastian Hofmann, Michael Schlüter, Ralf Takors   +5 more
wiley   +1 more source

Growth of Vero E-6 cells on microcarriers in a cell bioreactor [PDF]

, 1990
L A White, Edwin W. Ades
openalex   +1 more source

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndhairo, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Biotechnological production processes are sustainable approaches for the production of biobased components such as amino acids for food and feed industry. Scale‐up from ideal lab‐scale bioreactors to large‐scale processes is often accompanied by loss in productivity.
Manh Dat Hoang, Dieu Thi Doan, Marlen Schmidt, Harald Kranz, Andreas Kremling, Anna‐Lena Heins   +5 more
wiley   +1 more source

Flux Sampling Suggests Metabolic Signatures of High Antibody‐Producing CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Chinese hamster ovary (CHO) cells remain the industry standard for producing numerous therapeutic proteins, particularly monoclonal antibodies (mAbs). However, achieving higher recombinant protein titers remains an ongoing challenge and a fundamental understanding of the cellular mechanism driving improved bioprocess performance remains ...
Kate E. Meeson, Joanne Watson, Susan Rosser, Ellie Hawke, Andrew Pitt, Tessa Moses, Leon Pybus, Magnus Rattray, Alan J. Dickson, Jean‐Marc Schwartz   +9 more
wiley   +1 more source

Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Acetobacterium woodii is known to produce mainly acetate from CO2 and H2, but the production of higher value chemicals is desired for the bioeconomy. Using chain‐elongating bacteria, synthetic co‐cultures have the potential to produce longer‐chained products such as caproic acid.
Jan Herzog, Alexander Mook, Lotta Guhl, Miriam Bäumler, Matthias H. Beck, Dirk Weuster‐Botz, Frank R. Bengelsdorf, An‐Ping Zeng   +7 more
wiley   +1 more source

Design challenges for space bioreactors [PDF]

The design of bioreactors for operation under conditions of microgravity presents problems and challenges. Absence of a significant body force such as gravity can have profound consequences for interfacial phenomena. Marangoni convection can no longer be
Petersen, G. R., Seshan, P. K.
core   +1 more source

Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, EarlyView.
The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale, Luisa Blöbaum, Alexander Grünberger, Lisbeth Olsson   +3 more
wiley   +1 more source

FAIR research data management as community approach in bioengineering

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Research data management (RDM) requires standards, policies, and guidelines. Findable, accessible, interoperable, and reusable (FAIR) data management is critical for sustainable research. Therefore, collaborative approaches for managing FAIR‐structured data are becoming increasingly important for long‐term, sustainable RDM.
Martina Rehnert, Ralf Takors
wiley   +1 more source

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source