Results 81 to 90 of about 3,454,458 (298)

Efomycins K and L From a Termite-Associated Streptomyces sp. M56 and Their Putative Biosynthetic Origin

Frontiers in Microbiology, 2019
Two new elaiophylin derivatives, efomycins K (1) and L (2), and five known elaiophylin derivatives (3–7) were isolated from the termite-associated Streptomyces sp. M56. The structures were determined by 1D and 2D NMR and HR-ESIMS analyses and comparative
Jonathan L. Klassen, Seoung Rak Lee, Michael Poulsen, Christine Beemelmanns, Ki Hyun Kim   +4 more
doaj   +1 more source

Protocols for yTREX/Tn5‐based gene cluster expression in Pseudomonas putida

Microbial Biotechnology, 2020
Summary Bacterial gene clusters, which represent a genetic treasure trove for secondary metabolite pathways, often need to be activated in a heterologous host to access the valuable biosynthetic products.
Robin Weihmann, Andreas Domröse, Thomas Drepper, Karl‐Erich Jaeger, Anita Loeschcke   +4 more
doaj   +1 more source

Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction [PDF]

, 2005
Rückert C, Koch DJ, Rey DA, et al. Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction. BMC Genomics. 2005;6(1): 121.Background: Corynebacterium glutamicum
Rey, DA, Rückert, Christian ; https://orcid.org/, Albersmeier, Andreas, Koch, DJ, Mormann, S, Pühler, Alfred ; https://orcid.org/, Kalinowski, Jörn   +6 more
core   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Biosynthetic Gene Cluster for the Polyenoyltetramic Acid α-Lipomycin [PDF]

Antimicrobial Agents and Chemotherapy, 2006
ABSTRACT The gram-positive bacterium Streptomyces aureofaciens Tü117 produces the acyclic polyene antibiotic α-lipomycin. The entire biosynthetic gene cluster ( lip gene cluster) was cloned and characterized.
Bihlmaier, C., Welle, E., Hofmann, C., Welzel, K., Vente, A., Breitling, E., Müller, M., Glaser, S., Bechthold, A.   +8 more
openaire   +3 more sources

MIBiG 2.0: a repository for biosynthetic gene clusters of known function [PDF]

, 2019
Fueled by the explosion of (meta)genomic data, genome mining of specialized metabolites has become a major technology for drug discovery and studying microbiome ecology.
Tracanna, V., Selem-Mojica, N., Weber, T., Navarro-Muñoz, J. C., van Santen, J. A., Lund, G., Medema, M. H., Terlouw, B. R., Collemare, J., Shaw, S., Andreu, V. P., Sisto, A. C., Duran, H. G. S., Charkoudian, L. K., Robinson, S. L., Epstein, S. C., Kautsar, S. A., Blin, K., van der Hooft, J. J. J., Alanjary, M., Linington, R. G.   +20 more
core   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Identification and engineering of 32 membered antifungal macrolactone notonesomycins

Microbial Cell Factories, 2020
Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186.
Falicia Goh, Mingzi M. Zhang, Tian Ru Lim, Kia Ngee Low, Choy Eng Nge, Elena Heng, Wan Lin Yeo, Fernanda L. Sirota, Sharon Crasta, Zann Tan, Veronica Ng, Chung Yan Leong, Huibin Zhang, Alexander Lezhava, Swaine L. Chen, Shawn S. Hoon, Frank Eisenhaber, Birgit Eisenhaber, Yoganathan Kanagasundaram, Fong T. Wong, Siew Bee Ng   +20 more
doaj   +1 more source

Discovery of the lomaiviticin biosynthetic gene cluster in Salinispora pacifica [PDF]

Tetrahedron, 2014
The lomaiviticins are a family of cytotoxic marine natural products that have captured the attention of both synthetic and biological chemists due to their intricate molecular scaffolds and potent biological activities. Here we describe the identification of the gene cluster responsible for lomaiviticin biosynthesis in Salinispora pacifica strains DPJ ...
Jeffrey E, Janso, Brad A, Haltli, Alessandra S, Eustáquio, Kerry, Kulowski, Abraham J, Waldman, Li, Zha, Hitomi, Nakamura, Valerie S, Bernan, Haiyin, He, Guy T, Carter, Frank E, Koehn, Emily P, Balskus   +11 more
openaire   +2 more sources

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source