Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system [PDF]
Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody ...
Andrew Thompsett +22 more
core +1 more source
Background The amino acid-producing Gram-positive Corynebacterium glutamicum is auxotrophic for biotin although biotin ring assembly starting from the precursor pimeloyl-CoA is still functional.
Schneider Jens +6 more
doaj +1 more source
Impedimetric aptasensors without signal amplification exhibit poor sensitivity for the detection of small molecule contaminants. In this work, biotinylated nanoparticles of biotin-FNPs were readily prepared by the self-assembly of biotin-phenylalanine ...
Ming La +6 more
doaj +1 more source
The Crystal Structure of Monovalent Streptavidin. [PDF]
The strong interaction between streptavidin (SA) and biotin is widely utilized in biotechnological applications. A SA variant, monovalent SA, was developed with a single and high affinity biotin-binding site within the intact tetramer.
Biswas, Sangita +3 more
core +1 more source
Effects of Biotin-Avidin Interactions on Hydrogel Swelling
Affinity interactions between the small molecule biotin and the protein avidin have been used extensively to functionalize biomaterials. More recently, researchers have leveraged the changes in biotin-avidin affinity that occur upon biotin conjugation to
Talaial B. Alina +3 more
doaj +1 more source
Impaired biotin status in anticonvulsant therapy [PDF]
In 264 epileptics undergoing long-term therapy with anticonvulsants, significantly reduced plasma biotin levels were found compared with a normal control group: 74% of the epileptics had biotin levels for those treated with sodium valproate were higher ...
Bonjour +7 more
core +1 more source
Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels [PDF]
A method is described for indirect electron microscopic visualization and mapping of tRNA and other short transcripts hybridized to DNA. This method depends upon the attachment of the electron-dense protein ferritin to the RNA, the binding being mediated
Angerer, Lynne M. +4 more
core +2 more sources
Biotin interference with laboratory test results [PDF]
Introduction: Biotin interference in laboratory tests is a growing concern, particularly due to the widespread use of biotin not only as a therapeutic agent but also as a supplement in the cosmetic industry.
Strilić David Z. +2 more
doaj +1 more source
Effect of supplementation of biotin in total mixed ration of dairy cows on rumen fermentation characteristics by in vitro gas production technique [PDF]
An experiment was conducted to assess the effect of supplementation of biotin on rumen fermentation parameters and microbial biomass production by in vitro gas production technique. Biotin was supplemented to the substrate - total mixed ration (TMR) at
K. Jasmine Rani +5 more
doaj +1 more source
Effects of peripartum biotin supplementation of dairy cows on milk production and milk composition with emphasis on fatty acids profile [PDF]
Forty Holstein dairy cows receiving a 38% concentrate diet based on maize silage were assigned to either a control group, either a biotin group, receiving 20 mg of biotin per day from 15 days before expected calving date and for 120 days after calving ...
Enjalbert, Francis +2 more
core +1 more source

