Results 11 to 20 of about 338,983 (344)
Biotin, a universal and essential cofactor: Synthesis, ligation and regulation.
FEMS Microbiology Reviews, 2021 Biotin is a covalently attached enzyme cofactor required for intermediary metabolism in all three domains of life. Several important human pathogens (e.g. Mycobacterium tuberculosis) require biotin synthesis for pathogenesis.
Chaiyos Sirithanakorn, J. Cronan
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Ligand specificity of group I biotin protein ligase of Mycobacterium tuberculosis. [PDF]
PLoS ONE, 2008 BACKGROUND: Fatty acids are indispensable constituents of mycolic acids that impart toughness & permeability barrier to the cell envelope of M. tuberculosis.
Sudha Purushothaman +4 more
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Biotin's Lessons in Drug Design.
Journal of Medicinal Chemistry, 2021 At the heart of drug design is the discovery of molecules that bind with high affinity to their drug targets. Biotin forms the strongest known noncovalent ligand-protein interactions with avidin and streptavidin, achieving femtomolar and picomolar ...
D. McConnell
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Sensors, 2023 Biotin, or vitamin B7, is essential for metabolic reactions. It must be obtained from external sources such as food and biotin/vitamin supplements because it is not biosynthesized by mammals.
Fatema Kaladari +4 more
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Streptavidin/biotin: Tethering geometry defines unbinding mechanics
Science Advances, 2020 Single-molecule force spectroscopy reveals how the mechanical stability of a widely used biomolecular complex varies fourfold. Macromolecules tend to respond to applied forces in many different ways. Chemistry at high shear forces can be intriguing, with
Steffen M. Sedlak +3 more
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BMC Microbiology, 2012 Background The amino acid-producing Gram-positive Corynebacterium glutamicum is auxotrophic for biotin although biotin ring assembly starting from the precursor pimeloyl-CoA is still functional.
Schneider Jens +6 more
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Electrochemistry Communications, 2020 Impedimetric aptasensors without signal amplification exhibit poor sensitivity for the detection of small molecule contaminants. In this work, biotinylated nanoparticles of biotin-FNPs were readily prepared by the self-assembly of biotin-phenylalanine ...
Ming La +6 more
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Journal of Cell Biology, 2012 Proximity-dependent biotin identification (BioID) is a new approach making use of biotin ligase fusion proteins for the identification of both interacting and neighboring proteins in their native cellular environment.
K. Roux, D. Kim, M. Raida, B. Burke
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AACC Guidance Document on Biotin Interference in Laboratory Tests.
The Journal of Applied Laboratory Medicine, 2020 BACKGROUND Laboratory tests that use streptavidin-biotin binding mechanisms have the potential to be affected by high circulating biotin concentrations, which would produce positive and negative interference in biotinylated competitive and noncompetitive
Danni Li +4 more
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Mechanisms of biotin-regulated gene expression in microbes
Synthetic and Systems Biotechnology, 2016 Biotin is an essential micronutrient that acts as a co-factor for biotin-dependent metabolic enzymes. In bacteria, the supply of biotin can be achieved by de novo synthesis or import from exogenous sources.
J. Satiaputra +3 more
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