Results 81 to 90 of about 488,335 (365)
Sensors, 2013 A cantilever-based protein biosensor has been developed providing a customizable multilayer platform for the detection of antibodies. It consists of a biotin-terminated PEG layer pre-functionalized on the gold-coated cantilever surface, onto which ...
Christoph Gerber +5 more
doaj +1 more source
An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]
, 2013 Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Butt, Louise +9 more
core +3 more sources
A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner
Molecular Oncology, EarlyView.We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp +13 more
wiley +1 more source
Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
Nature Methods, 2017 Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide ...
N. Udeshi +12 more
semanticscholar +1 more source
Development and Evaluation of an Anti-Biotin Interference Method in Biotin-Streptavidin Immunoassays
Diagnostics, 2022 The strong non-covalent interaction between biotin and streptavidin places streptavidin-based assays, used by many laboratories, at an increased risk of interference by biotin.
Dong Liu +7 more
doaj +1 more source
Magnetic Nanoparticle Relaxation Dynamics-based Magnetic Particle Spectroscopy (MPS) for Rapid and Wash-free Molecular Sensing [PDF]
, 2019 Magnetic nanoparticles (MNPs) have been extensively used as contrasts and tracers for bioimaging, heating sources for tumor therapy, carriers for controlled drug delivery, and labels for magnetic immunoassays. Here, we describe a MNP relaxation dynamics-based magnetic particle spectroscopy (MPS) method for the quantitative detection of molecular ...
arxiv +1 more source
Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]
, 1990 A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Anna Jauch +22 more
core +1 more source
Molecular Oncology, EarlyView.Human cytomegalovirus infection is common in normal prostate epithelium, prostate tumor tissue, and prostate cancer cell lines. CMV promotes cell survival, proliferation, and androgen receptor signaling. Anti‐CMV pharmaceutical compounds in clinical use inhibited cell expansion in prostate cancer models in vitro and in vivo, motivating investigation ...
Johanna Classon +13 more
wiley +1 more source
Synthetic mimics of biotin/(strept)avidin.
Chemical Society Reviews, 2017 Biotin/(strept)avidin self-assembly is a powerful platform for nanoscale fabrication and capture with many different applications in science, medicine, and nanotechnology. However, biotin/(strept)avidin self-assembly has several well-recognized drawbacks
Wenqi Liu +3 more
semanticscholar +1 more source
On-chip label-free biosensing based on active whispering gallery mode resonators pumped by a light-emitting diode [PDF]
, 2019 Biosensing based on whispering-gallery mode (WGM) resonators has been continuously studied with great attention due to its excellent sensitivity guaranteeing the label-free detection. However, its practical impact is insignificant to date despite notable achievements in academic research.
arxiv +1 more source