Results 251 to 260 of about 143,695 (288)

The HaMYB22–HaGST3.2 module mediates salt stress response in sunflower

Journal of Integrative Plant Biology, EarlyView.
The transcription factors HaMYB22, HaMYB120, and HaMYB121 cooperatively activate the transcription of the glutathione S‐transferase gene HaGST3.2, thereby facilitating the removal of reactive oxygen species induced by salt stress in sunflowers and improving their salt tolerance.
Siqi Zhang, Yuliang Han, Qixiu Huang, Weijun Guo, Shurui Dong, Xinxin Li, Qian Zhang, Juncheng Zhang, Yijun Meng, Zhonghua Lei, Maohong Cai, Tao Chen   +11 more
wiley   +1 more source

The transcription factors TaABI4 and TaNAC‐A1 fine‐tune TaCYP94‐A1 transcription to modulate seed dormancy in wheat

Journal of Integrative Plant Biology, EarlyView.
A module involving the transcription factors TaABI4 and TaNAC‐A1, and the cytochrome P450 TaCYP94‐A1 precisely regulates seed dormancy levels through plant hormone crosstalk, providing targets for improving resistance to pre‐harvest sprouting in wheat.
Litian Zhang, Yuhui Fang, Yunfei Zhou, Xianlai Nie, Shujun Cao, Wei Gao, Yu Wang, Lianghua Dai, Lin Ye, Jie Lu, Chuanxi Ma, Cheng Chang, Haiping Zhang   +12 more
wiley   +1 more source

Transcriptional adapter ADA2 regulates yield and end‐use quality through liquid–liquid phase separation in wheat

Journal of Integrative Plant Biology, EarlyView.
The transcriptional adapter ADA2 interacts with the histone acetyltransferase GCN5 to regulate starch and storage protein content in wheat grains through histone acetylation. ADA2 undergoes phase separation and is modulated by GCN5, affecting histone acetyltransferase activity and gene transcription.
Xiaobang Zhang, Ruijie Zhang, Yuhan Zhang, Tao Yang, Longfei Wu, Yuan Cao, Jun Xiao, Jigang Li, Xin Gao, Xinyou Cao, Jie Liu, Mingming Xin, Yuan Zong, Zhongfu Ni, Qixin Sun, Yingyin Yao   +15 more
wiley   +1 more source

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On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains

Journal of Biotechnology, 2019
The CRISPR-Cas9 system is a new tool that has been extensively used for genome editing. The system is composed of a Cas9 endonuclease, which has the function of cleaving DNA at a specific site, and a guide RNA (gRNA), which contains the sequence of the cleavage site that is the target of editing.
Gabriela Pannunzio, Carmignotto, Adriano Rodrigues, Azzoni   +1 more
openaire   +4 more sources

The toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3)

Protein Expression and Purification, 2004
Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system.
Laurence, Dumon-Seignovert, Guillaume, Cariot, Laurent, Vuillard   +2 more
openaire   +4 more sources

Genome Sequences of Escherichia coli B strains REL606 and BL21(DE3)

Journal of Molecular Biology, 2009
Escherichia coli K-12 and B have been the subjects of classical experiments from which much of our understanding of molecular genetics has emerged. We present here complete genome sequences of two E. coli B strains, REL606, used in a long-term evolution experiment, and BL21(DE3), widely used to express recombinant proteins.
Jeong, H Jeong, Haeyoung, Barbe, V Barbe, Valerie, Lee, CH Lee, Choong Hoon, Vallenet, D Vallenet, David, Yu, DS Yu, Dong Su, Choi, SH Choi, Sang-Haeng, Couloux, A Couloux, Arnaud, Lee, SW Lee, Seung-Won, Yoon, SH Yoon, Sung Ho, Cattolico, L Cattolico, Laurence, Hur, CG Hur, Cheol-Goo, Park, HS Park, Hong-Seog, Segurens, B Segurens, Beatrice, Kim, SC Kim, Sun-Chang, Oh, TK Oh, Tae Kwang, Lenski, RE Lenski, Richard E., Studier, FW Studier, F. William, Daegelen, P Daegelen, Patrick, Kim, JF Kim, Jihyun F.   +18 more
openaire   +5 more sources

Construction of a synthetic YdbK-dependent pyruvate:H2 pathway in Escherichia coli BL21(DE3)

Metabolic Engineering, 2009
A synthetic pyruvate:H(2) pathway was constructed in Escherichia coli BL21(DE3) by co-expression of six proteins: E. coli YdbK, Clostridium pasteurianum [4Fe-4S]-ferredoxin, and Clostridium acetobutylicum HydF, HydE, HydG, and HydA. The effect of cofactor addition and host strain on H(2) yield and fermentation product accumulation was studied, together
M Kalim, Akhtar, Patrik R, Jones
openaire   +4 more sources

The dynamic changes of glycogen molecular structure in Escherichia coli BL21(DE3)

Carbohydrate Polymers, 2021
Diurnal alteration of glycogen molecular structure has been identified in healthy mice. Recently, both fragile (disintegration in dimethyl sulfoxide) and stable (not disintegrating in DMSO) glycogen particles were found in Escherichia coli. However, how glycogen structure changes dynamically in E. coli is not clear.
Mengmeng Wang, Qinghua Liu, Fen Li, Jiawei Tang, Xuesong Xiong, Yingying Yang, Pei Ju, Ziyi Wang, Robert G. Gilbert, Liang Wang   +9 more
openaire   +2 more sources

Optimized expression and refolding of human keratoepithelin in BL21 (DE3)

Protein Expression and Purification, 2004
Keratoepithelin (KE) is an extracellular protein participating in cell adhesion and differentiation. Mutations of the KE gene (on 5q31 in humans) cause deposition of abnormal proteins (amyloid and non-amyloid) in corneal stroma and lead to several corneal dystrophies in humans.
Ching, Yuan, Janice M, Reuland, Lyndon, Lee, Andrew J W, Huang   +3 more
openaire   +2 more sources