Results 261 to 270 of about 143,695 (288)

Overexpression of a lethal methylase, M.TneDI, in E. coli BL21(DE3)

Biotechnology Letters, 2014
A pET-based vector pDH21 expressing the methylase, M.TneDI (recognizing CGCG) from Thermotoga was constructed, and transformed into E. coli BL21(DE3). Despite E. coli BL21(DE3) being McrBC positive, 30 transformants were isolated, which were suspected to be McrBC(-) mutants. The overexpression of M.TneDI was verified by SDS-PAGE analysis.
Hui, Xu, Dongmei, Han, Zhaohui, Xu
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Multiple Stressor-Induced Proteome Responses of Escherichia coli BL21(DE3)

Journal of Proteome Research, 2008
Through 2-DE based quantitative proteomics, the dynamic characteristics of overall proteome profiles of Escherichia coli BL21(DE3) were systematically analyzed in the presence of four different stressors. Dithiothreitol and 2-hydroxyethyl disulfide are a reducing and an oxidizing agent, respectively, which disturb the redox balance in cytoplasm, while ...
Kyung-Yeon, Han, Jin-Seung, Park, Hyuk-Seong, Seo, Keum-Young, Ahn, Jeewon, Lee   +4 more
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Expression and Purification of Tetanus Toxin Fragment C in Escherichia coli BL21(DE3)

Protein & Peptide Letters, 2020
Background: Tetanus is an infectious disease caused by Clostridium secreting tetanus toxin in anaerobic environment. The fragment C of Tetanus toxin (TTc) has been widely studied as a candidate vaccine to replace the existing tetanus toxoid vaccine. Objective: In this study, we established a simple method to purify recombinant protein TTc with ion ...
Pengdi, Chai, Xiuying, Pu, Jianqiang, Li, Xiaoyu, Xia, Jun, Ge, Amiao, Luo, Hui, Su, Weijie, Zhang, Jianzhong, Ma   +8 more
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A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

2021
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations.
Julian, Kopp, Oliver, Spadiut
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Escherichia coli BL21(DE3) chromosome contains a group II capsular gene cluster

Gene, 2006
During our study of de novo synthesis of Escherichia coli K1 capsular polysaccharides, we found that E. coli BL21(DE3) has a capsular gene cluster, similar to those of group II capsular E. coli strains. Analysis of the nucleotide sequence of the E. coli BL21(DE3) gene cluster showed homologues to all group II regions 1 and 3 genes and the presence of ...
Ekaterina N, Andreishcheva, Willie F, Vann   +1 more
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Discovery of novel feruloyl esterase activity of BioH in Escherichia coli BL21(DE3)

Biotechnology Letters, 2016
To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3.The gene encoding BioH was cloned and overexpressed in E. coli. The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ...
Le, Kang, Yajun, Bai, Yujie, Cai, Xiaohui, Zheng   +3 more
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Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3)

Applied Microbiology and Biotechnology, 2018
A novel method involving ethanol-induced increase in the heterologous recombinant protein expression in E. coli cells was commonly used in recent studies. However, the detailed mechanism of this method is still to be revealed. This work used comparative transcriptomic analysis and numerous experiments to uncover the mechanism of ethanol effects on the ...
Hongchen Zheng, Zhenxiao Yu, Wenju Shu, Xiaoping Fu, Xingya Zhao, Shibin Yang, Ming Tan, Jianyong Xu, Yihan Liu, Hui Song   +9 more
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STATISTICAL OPTIMIZATION OF GREEN FLUORESCENT PROTEIN PRODUCTION FROMEscherichia coliBL21(DE3)

Preparative Biochemistry and Biotechnology, 2012
An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design.
Few Ne, Chew, Wen Siang, Tan, Huey Chern, Boo, Beng Ti, Tey   +3 more
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Production of 1,3-Propanediol from Glucose by Recombinant Escherichia coli BL21(DE3)

Biotechnology and Bioprocess Engineering, 2018
A range of recombinant strains of Escherichia coli were developed to produce 1,3-propanediol (1,3-PDO), an important C3 diol, from glucose. Two modules, the glycerol-producing pathway converting dihydroxyacetone phosphate to glycerol and the 1,3-PDO-producing pathway converting glycerol to 1,3-PDO, were introduced into E. coli.
Lee, Jae Hyeon, Lama, Suman, Kim, Jung Rae, Park, Sung Hoon   +3 more
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Rapid label‐free quantitative analysis of the E. coli BL21(DE3) inner membrane proteome

PROTEOMICS, 2015
Biological membranes define cells and cellular compartments and are essential in regulating bidirectional flow of chemicals and signals. Characterizing their protein content therefore is required to determine their function, nevertheless, the comprehensive determination of membrane‐embedded sub‐proteomes remains challenging.
Malvina, Papanastasiou, Georgia, Orfanoudaki, Nikos, Kountourakis, Marina, Koukaki, Marios Frantzeskos, Sardis, Michalis, Aivaliotis, Konstantinos C, Tsolis, Spyridoula, Karamanou, Anastassios, Economou   +8 more
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