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[Cloning and expression of caiDE genes in E. coli BL21(DE3)].
Wei sheng wu xue bao = Acta microbiologica Sinica, 2004The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2.
J, Jiang, S, Duan, H, Zhang
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2019
The E. coli strain BL21 (DE3) is widely applied for the synthesis of recombinant products using the bacteriophage T7 polymerase-base expression systems. The level of target protein production in E. coli cells has been recently shown to depend on the type of carbon source in the growth media and the producer metabolic properties, especially by acetate ...
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The E. coli strain BL21 (DE3) is widely applied for the synthesis of recombinant products using the bacteriophage T7 polymerase-base expression systems. The level of target protein production in E. coli cells has been recently shown to depend on the type of carbon source in the growth media and the producer metabolic properties, especially by acetate ...
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Gezielte Optimierung von Escherichia coli BL21(DE3)
BIOspektrum, 2013Stephan Noll +7 more
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[High cell density culture of phosphotransacetylase mutants of Escherichia coli BL21(DE3)].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2001Cell culture, organic acid production and foreign protein (TNF) expression of E. coli BL21(DE3) and its pta mutant were investigated. Under shaking conditions, TNF expression in pta mutant increased by 23%. During the fed-batch culture without limitation of specific growth rates, the mutant reached a cell density as high as 32.5 g(DCW)/L and total TNF ...
W C, Zhang +4 more
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