Purified human BRCA2 stimulates RAD51-mediated recombination. [PDF]
Nature, 2010 Mutation of the breast cancer susceptibility gene, BRCA2, leads to breast and ovarian cancers. Mechanistic insight into the functions of human BRCA2 has been limited by the difficulty of isolating this large protein (3,418 amino acids).
Carreira, Aura +2 more
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Abnormal Cytokinesis in Cells Deficient in the Breast Cancer Susceptibility Protein BRCA2
Science, 2004 Germ-line mutations inactivating BRCA2 predispose to cancer. BRCA2-deficient cells exhibit alterations in chromosome number (aneuploidy), as well as structurally aberrant chromosomes. Here, we show that BRCA2 deficiency impairs the completion of cell division by cytokinesis.
Daniels, Matthew J +3 more
openaire +5 more sources
Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein. [PDF]
Mol Cell, 2023 Belan O +10 more
europepmc +2 more sources
On the Mechanism of Hyperthermia-Induced BRCA2 Protein Degradation. [PDF]
Cancers (Basel), 2019 The DNA damage response (DDR) is a designation for a number of pathways that protects our DNA from various damaging agents. In normal cells, the DDR is extremely important for maintaining genome integrity, but in cancer cells these mechanisms counteract therapy-induced DNA damage.
van den Tempel N +8 more
europepmc +6 more sources
Functional Restoration of BRCA2 Protein by Secondary BRCA2 Mutations in BRCA2 -Mutated Ovarian Carcinoma [PDF]
Cancer Research, 2009 Abstract Acquired platinum resistance is a serious problem in the treatment of ovarian carcinomas. However, the mechanism of the drug resistance has not been elucidated. Here, we show functional significance of restoration of BRCA2 protein by secondary BRCA2 mutations in acquired drug resistance of BRCA2-mutated ovarian carcinoma ...
Sakai, Wataru +9 more
openaire +3 more sources
The BRCA2-Interacting Protein BCCIP Functions in RAD51 and BRCA2 Focus Formation and Homologous Recombinational Repair [PDF]
Molecular and Cellular Biology, 2005 Homologous recombinational repair (HRR) of DNA damage is critical for maintaining genome stability and tumor suppression. RAD51 and BRCA2 colocalization in nuclear foci is a hallmark of HRR. BRCA2 has important roles in RAD51 focus formation and HRR of DNA double-strand breaks (DSBs). We previously reported that BCCIPalpha interacts with BRCA2. We show
Huimei, Lu +7 more
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Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2 [PDF]
Oncogene, 2003 Mutations in the BRCA1 and BRCA2 genes predispose women to familial, early-onset breast cancer. Both the BRCA1 and BRCA2 proteins appear to function in the homologous recombination pathway of DNA double-strand break repair. Both BRCA1 and BRCA2 have also been implicated in transcription by RNA polymerase II, for both proteins have domains which, when ...
Johnson M S, Wong +2 more
openaire +2 more sources
A new interaction between BRCA2 and DDX5 promotes the repair of DNA breaks at transcribed chromatin
Molecular & Cellular Oncology, 2021 In a recent report, we have revealed a new interaction between the BRCA2 DNA repair associated protein (BRCA2) and the DEAD-box helicase 5 (DDX5) at DNA breaks that promotes unwinding DNA-RNA hybrids within transcribed chromatin and favors repair ...
Belen Gómez-González +3 more
doaj +1 more source
Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA. [PDF]
, 2010 BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and
Doty, Tammy +3 more
core +2 more sources
BMC Molecular and Cell Biology, 2021 Background Breast cancer 2, early onset (BRCA2) is a tumor suppressor gene. The protein encoded by this gene plays an important role in homologous recombination (HR)-mediated DNA repair. Deleterious mutations in BRCA2 and downregulation of its expression
Yasunaga Yoshikawa +4 more
doaj +1 more source