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BRET Q-Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase-Dye-Antibody Fusion with Enhanced Response.

Analytical Chemistry, 2021
A quenchbody (Q-body) is an immunosensor comprising an antibody fragment containing an antigen-binding site that is site-specifically labeled with a fluorescent dye.
Riho Takahashi   +3 more
semanticscholar   +1 more source

BRET Nano Q-Body: A Nanobody-Based Ratiometric Bioluminescent Immunosensor for Point-of-Care Testing

ACS Sensors
We developed a nanobody-based homogeneous bioluminescent immunosensor to achieve a one-pot detection for point-of-care testing (POCT). This immunosensor was named BRET nano Q-body as its emission color changes via bioluminescence resonance energy ...
Tetsuya Kitaguchi   +2 more
exaly   +2 more sources

Engineering BRET-Sensor Proteins

2017
FRET-sensors have become important tools for intracellular imaging, but their dependence on external illumination presents some limitations, such as photobleaching and phototoxicity, which limit measurements over extended periods of time. Fluorescence measurements also suffer from autofluorescence and light scattering, which hampers in vivo imaging and
Remco Arts   +2 more
openaire   +4 more sources

Bret Easton Ellis

2004
Revisione e aggiornamento della voce dallo stesso titolo pubblicata in Voci dagli Stati Uniti. Prosa & Poesia & Teatro del Secondo Novecento, C. Ricciardi, V.M. De Angelis, a cura di, Roma: Centro stampa d'Ateneo, 2004, pp. 577-586.
openaire   +3 more sources

A red-shifted Bioluminescence Resonance Energy Transfer (BRET) biosensing system for rapid measurement of plasmin activity in human plasma.

Analytica Chimica Acta, 2020
Proteases are key signalling molecules for many physiological processes and their dysregulation is implicated in the progression of a range of diseases. Sensitive methods to measure protease activities in complex biological samples are critical for rapid
F. Weihs, Alex Peh, H. Dacres
semanticscholar   +1 more source

Development and characterisation of a compact device for rapid real-time-on-chip detection of thrombin activity in human serum using bioluminescence resonance energy transfer (BRET).

Biosensors & bioelectronics, 2020
Bioluminescence resonance energy transfer (BRET) is a sensitive optical detection method that can monitor changes in the relative orientation and the physical proximity of molecules in real-time.
F. Weihs   +5 more
semanticscholar   +1 more source

Byron and Bret Harte

The Byron Journal, 2004
Bret Harte (1836-1902), one of the most popular American writers of the late nineteenth century, alluded to Byron in his writings more often than he referred to any other poet except Longfellow and perhaps Browning. As early as September 1860, he noted in an autobiographical essay that in adolescence he had 'read Byron, Moore, and Bulwer's early works,
openaire   +1 more source

β-arrestin-2 BRET biosensors detect different β-arrestin-2 conformations in interaction with GPCRs.

ACS Sensors, 2019
β-arrestins are critical regulators of G protein-coupled receptors (GPCRs) that desensitize G protein signaling, promote receptor internalization and initiate signaling on their own.
Atsuro Oishi, J. Dam, R. Jockers
semanticscholar   +1 more source

Bret Easton Ellis

2015
Bereits als College-Student 1985 erster Romanerfolg; wurde danach als ›Stimme‹ seiner Generation gehandelt; 1987 Umzug nach New York; 1991 Skandalerfolg mit American Psycho; gilt seitdem als Reprasentant des literarischen Tabubruchs und umstrittenster Vertreter des sogenannten ›Brat Pack‹, einer in Anlehnung an das ›Rat Pack‹ der 1950er Jahre ...
openaire   +1 more source

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