Results 201 to 210 of about 210,338 (264)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Interfacial pH Dynamics in Lattice-Nitrogen Protonation to Ammonia. [PDF]
J Am Chem SocYesudoss DK +3 more
europepmc +1 more source
Multi‑omics integration in osteoarthritis: Unraveling cell‑type‑specific gene‑metabolite networks for precision medicine (Review). [PDF]
Int J Mol MedYang Y +5 more
europepmc +1 more source
Direct Observation of Topologically Enhanced Interfacial Miscibility in Ring-Linear Polymers. [PDF]
ACS Cent SciKim SE +6 more
europepmc +1 more source
Data-driven identification of cell subtypes for single-cell transcriptomic data with Subtypist. [PDF]
Brief BioinformYao Y +7 more
europepmc +1 more source