Results 21 to 30 of about 17,904 (252)

Polarity of c-di-GMP synthesis and degradation

microLife, 2023
AbstractThe bacterial cell pole has long been recognized as a defined compartment for enzymatic activities that are important or even vital for the cell. Polarity of diguanylate cyclases and phosphodiesterases, enzymes that synthesize and degrade the second messenger c-di-GMP, has now been demonstrated for several bacterial systems.
Vanessa Kreiling, Kai M Thormann
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Visualization of c-di-GMP in multicellular Dictyostelium stages

Frontiers in Cell and Developmental Biology, 2023
The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold Dictyostelium discoideum among eukaryotes. Dictyostelium cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores.
Hayato Ide, Yukihisa Hayashida, Yusuke V. Morimoto, Yusuke V. Morimoto   +3 more
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Identification of Cyclic-di-GMP-Modulating Protein Residues by Bidirectionally Evolving a Social Behavior in Pseudomonas fluorescens

mSystems, 2022
Modulation of the intracellular cyclic di-GMP (c-di-GMP) pool is central to the formation of structured bacterial communities. Genome annotations predict the presence of dozens of conserved c-di-GMP catalytic enzymes in many bacterial species, but the ...
Collin Kessler, Wook Kim
doaj   +1 more source

Thiophosphate Analogs of c-di-GMP: Impact on Polymorphism [PDF]

Nucleosides, Nucleotides & Nucleic Acids, 2009
Seven phosphorothioate analogs of c-di-GMP (all diastereomers of mono-, di-, and trithiophosphates) were prepared to assess the impact of the thioate substitutions on c-di-GMP polymorphism using 1D (1)H and (31)P NMR, along with 2D NOESY and DOSY, for both the Na(+) and K(+) salts.
Jianwei, Zhao, Elizabeth, Veliath, Seho, Kim, Barbara L, Gaffney, Roger A, Jones   +4 more
openaire   +2 more sources

Multiple activities of c-di-GMP in Pseudomonas aeruginosa [PDF]

Nucleic Acids Symposium Series, 2009
Survival strategies of many bacterial pathogens, including Pseudomonas aeruginosa, are linked to their ability to form surface associated communities called biofilms. The biofilm life style allows these organisms to persist in various tissues, avoid clearance by innate host defences and significantly enhanced their resistance to antibiotics.
Stephen, Lory, Massimo, Merighi, Mamoru, Hyodo   +2 more
openaire   +2 more sources

Mutant structure of metabolic switch protein in complex with monomeric c-di-GMP reveals a potential mechanism of protein-mediated ligand dimerization

Scientific Reports, 2023
Bacterial second messengers c-di-GMP and (p)ppGpp have broad functional repertoires ranging from growth and cell cycle control to the regulation of biofilm formation and virulence.
Badri Nath Dubey, Viktoriya Shyp, Geoffrey Fucile, Holger Sondermann, Urs Jenal, Tilman Schirmer   +5 more
doaj   +1 more source

A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins

Bio-Protocol, 2015
Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many processes in bacteria including biofilm formation, motility, and virulence (Hengge, 2009). Analysis of c-di-GMP binding properties of bacterial proteins is an important step to
Disha Srivastava, Christopher Waters
doaj   +1 more source

Protein Deacetylase CobB Interplays with c-di-GMP [PDF]

SSRN Electronic Journal, 2018
Abstract As a ubiquitous bacterial secondary messenger, c-di-GMP plays key regulatory roles in processes such as bacterial motility and transcription regulation. CobB is the Sir2 family protein deacetylase that controls energy metabolism, chemotaxis and DNA supercoiling in many bacteria.
Xu, Zhaowei, Zhang, Hainan, Zhang, Xingrun, Liu, Chengxi, Jiang, Hewei, Wu, Fanlin, Qian, Lili, Czajkowsky, Daniel M., Guo, Shujuan, Bi, Lijun, Wang, Shihua, Li, Haitao, Tan, Minjia, Feng, Lei, Hou, Jingli, Tao, Sheng-ce   +15 more
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Evolution of a σ–(c-di-GMP)–anti-σ switch [PDF]

Proceedings of the National Academy of Sciences, 2021
SignificanceDiverse bacterial lifestyle transitions are controlled by the nucleotide second messenger c-di-GMP, including virulence, motility, and biofilm formation. To control such fundamentally distinct processes, the set of genes under c-di-GMP control must have gone through several shifts during bacterial evolution.
Maria A. Schumacher, Kelley A. Gallagher, Neil A. Holmes, Govind Chandra, Max Henderson, David T. Kysela, Richard G. Brennan, Mark J. Buttner   +7 more
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Cyclic Diguanylate Regulates Virulence Factor Genes via Multiple Riboswitches in Clostridium difficile

mSphere, 2018
The intracellular signaling molecule cyclic diguanylate (c-di-GMP) regulates many processes in bacteria, with a central role in controlling the switch between motile and nonmotile lifestyles.
Robert W. McKee, Carissa K. Harvest, Rita Tamayo   +2 more
doaj   +1 more source