Results 21 to 30 of about 362,233 (261)
Fluorescent sensors for imaging of interstitial calcium [PDF]
Nature Communications, 2023 Abstract Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues.
Ariel A. Valiente-Gabioud +8 more
openaire +5 more sources
A Novel Method to Quantify Calcium Response Pattern and Oscillation Using Fura2 and Acridine Orange
Journal of Pharmacological Sciences, 2004 To study calcium imaging data of cell populations that have various response patterns in peak amplitude and frequency of calcium oscillation in response to stimulation, comprehensive characterization based on statistical analysis of each response is ...
Mitsuhiro Morita +4 more
doaj +1 more source
Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging. [PDF]
PLoS ONE, 2014 For a comprehensive understanding of cellular processes and potential dysfunctions therein, an analysis of the ubiquitous intracellular second messenger calcium is of particular interest.
Karolina Jahn, Carsten Hille
doaj +1 more source
Calcium Sensor for Photoacoustic Imaging
Journal of the American Chemical Society, 2017 We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction ...
Sheryl Roberts +17 more
openaire +5 more sources
Bio-Protocol, 2017 Fertilization calcium waves are a conserved trigger for animal development; however, genetic analysis of these waves has been limited due to the difficulty of imaging in vivo fertilization.
Jun Takayama +2 more
doaj +1 more source
High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators
iScience, 2019 Summary: Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical ...
Urs A.T. Hofmann +6 more
doaj +1 more source
Multiphoton Intravital Calcium Imaging [PDF]
Current Protocols in Cytometry, 2018 AbstractMultiphoton intravital calcium imaging is a powerful technique that enables high‐resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2‐photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain.
openaire +2 more sources
CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data
BMC Neuroscience, 2022 Background Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced ...
Madhavi Tippani +9 more
doaj +1 more source
Dendritic spike saturation of endogenous calcium buffer and induction of postsynaptic cerebellar LTP. [PDF]
PLoS ONE, 2008 The architecture of parallel fiber axons contacting cerebellar Purkinje neurons retains spatial information over long distances. Parallel fiber synapses can trigger local dendritic calcium spikes, but whether and how this calcium signal leads to plastic ...
Marco Canepari, Kaspar E Vogt
doaj +1 more source
eLife, 2020 The ‘building-block’ model of inositol trisphosphate (IP3)-mediated Ca2+ liberation posits that cell-wide cytosolic Ca2+ signals arise through coordinated activation of localized Ca2+ puffs generated by stationary clusters of IP3 receptors (IP3Rs). Here,
Jeffrey T Lock, Ian Parker
doaj +1 more source