F1000Research, 2021 Techniques for calcium imaging were first demonstrated in the mid-1970s, whilst tools to analyse these markers of cellular activity are still being developed and improved today.
Miranda Robbins +3 more
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A Novel Method to Quantify Calcium Response Pattern and Oscillation Using Fura2 and Acridine Orange
Journal of Pharmacological Sciences, 2004 To study calcium imaging data of cell populations that have various response patterns in peak amplitude and frequency of calcium oscillation in response to stimulation, comprehensive characterization based on statistical analysis of each response is ...
Mitsuhiro Morita +4 more
doaj +1 more source
Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging. [PDF]
PLoS ONE, 2014 For a comprehensive understanding of cellular processes and potential dysfunctions therein, an analysis of the ubiquitous intracellular second messenger calcium is of particular interest.
Karolina Jahn, Carsten Hille
doaj +1 more source
A genetically encoded reporter of synaptic activity in vivo [PDF]
, 2009 To image synaptic activity within neural circuits, we tethered the genetically encoded calcium indicator (GECI) GCaMP2 to synaptic vesicles by fusion to synaptophysin. The resulting reporter, SyGCaMP2, detected the electrical activity of neurons with two
B Granseth +35 more
core +1 more source
Nonspecific, Reversible Inhibition of Voltage-Gated Calcium Channels by CaMKII Inhibitor CK59 [PDF]
, 2013 Investigation of kinase-related processes often uses pharmacological inhibition to reveal pathways in which kinases are involved. However, one concern about using such kinase inhibitors is their potential lack of specificity.
Kahrls, Andrew S., Mynlieff, Michelle
core +2 more sources
Multiphoton Intravital Calcium Imaging [PDF]
Current Protocols in Cytometry, 2018 AbstractMultiphoton intravital calcium imaging is a powerful technique that enables high‐resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2‐photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain.
openaire +2 more sources
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis [PDF]
, 2020 Spontaneous intracellular calcium activity can be observed in a variety of cell types and is proposed to play critical roles in a variety of physiological processes.
Ablondi, Eileen F. +7 more
core
FocusStack and StimServer: a new open source MATLAB toolchain for visual stimulation and analysis of two-photon calcium neuronal imaging data [PDF]
, 2014 Two-photon calcium imaging of neuronal responses is an increasingly accessible technology for probing population responses in cortex at single cell resolution, and with reasonable and improving temporal resolution. However, analysis of two-photon data is
Kampa, Björn M., Muir, Dylan R.
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Hybrid volumetric calcium imaging [PDF]
Nature Methods, 2019 Hybrid multiplexed sculpted light microscopy enables calcium imaging of volumes as large as half a cubic millimeter.
openaire +2 more sources
Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling. [PDF]
, 2014 One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment.
Bonora, M +9 more
core +3 more sources