Results 21 to 30 of about 2,798,160 (306)

Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain

eLife, 2020
1300 nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and perturbation to the sample.
Tianyu Wang, Chunyan Wu, Dimitre G Ouzounov, Wenchao Gu, Fei Xia, Minsu Kim, Xusan Yang, Melissa R Warden, Chris Xu   +8 more
doaj   +1 more source

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [PDF]

, 2013
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby.
Looger, Loren L, Tolö, Johan, Michael B Orger, Leon eLagnado, Tian, Lin, Pulver, Stefan R., Marvin, Jonathan S, Gottschalk, Alexander, Lin eTian, Leon Lagnado, Carreras Calderón, Nicole, Jasper eAkerboom, Kristen E Severi, Eric R Schreiter, Kristen E. Severi, Ronak ePatel, Alexander Gottschalk, Matthias ePrigge, Nicole Carreras Calderón, Kim, Douglas S, Lin Tian, Cornelia I Bargmann, Calderon, Nicole Carreras, Marvin, Jonathan S., Sebastian eKügler, Jasper Akerboom, Douglas S Kim, Douglas S. Kim, Orger, Michael B, Tsai-Wen eChen, Macklin, John J., Sebastian Wabnig, Tsai-Wen Chen, Elisabeth Fischer, Alexander eGottschalk, Elisabeth eFischer, Sarkisyan, Karen S, Marvin, Jonathan, Schreiter, Eric, Matthias Prigge, Wardill, Trevor J, Andrew eGordus, John J Macklin, Schüler, Christina, Trevor J. Wardill, Ronak Patel, Sebastian eWabnig, Severi, Kristen E., Nicole eCarreras Calderón, Severi, Kristen E, Loren L. Looger, Trevor J Wardill, Karen S. Sarkisyan, Christina Schüler, Fischer, Elisabeth, Patel, Ronak, Hegemann, Peter, Stefan R Pulver, Pulver, Stefan R, Kügler, Sebastian, Wardill, Trevor, Eric R. Schreiter, Loren L Looger, Wabnig, Sebastian, Jonathan S. Marvin, Johan Tolö, Sebastian Kügler, Schreiter, Eric R., Patel, Ronak Bhailal, Cornelia I. Bargmann, Sarkisyan, Karen S., Schueler, Christina, John J. Macklin, Andrew Gordus, Karen S Sarkisyan, Chen, Tsai-Wen, Bargmann, Cornelia I., Jonathan S Marvin, Looger, Loren L., Kim, Douglas S., Orger, Michael B., Schreiter, Eric R, Lagnado, Leon, Johan eTolö, Peter Hegemann, Orger, Michael, Prigge, Matthias, Bargmann, Cornelia I, Gordus, Andrew, Christina eSchüler, Stefan R. Pulver, Michael B. Orger, Wardill, Trevor J., Severi, Kristen, Bargmann, Cornelia, Peter eHegemann, Akerboom, Jasper, Macklin, John J, Pulver, Stefan R; id_orcid   +98 more
core   +1 more source

Imaging large cohorts of single ion channels and their activity [PDF]

, 2013
As calcium is the most important signaling molecule in neurons and secretory cells, amongst many other cell types, it follows that an understanding of calcium channels and their regulation of exocytosis is of vital importance.
Euan R. Brown, Katia eHiersemenzel, Brown, Euan R; id_orcid, Katia Hiersemenzel, Rory R. Duncan, Duncan, Rory R, Hiersemenzel, Katia, Rory R Duncan, Euan R Brown   +8 more
core   +1 more source

Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells

BioTechniques, 2018
Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics.
Igor Razlivanov, Teresa Liew, Eira Watts Moore, Alaa Al-Kathiri, Tayma Bartram, Dmitriy Kuvshinov, Anton Nikolaev   +6 more
doaj   +1 more source

Calcium Imaging and Electrophysiology of hippocampal Activity under Anesthesia and natural Sleep in Mice

Scientific Data, 2022
Measurement(s) brain activity measurement • pupil dilation Technology Type(s) two-photon calcium imaging • local field potential recording • videography Factor Type(s) calcium imaging data • local field potentials Sample Characteristic - Organism Mus ...
Andrey Formozov, Mattia Chini, Alexander Dieter, Wei Yang, Jastyn A. Pöpplau, Ileana L. Hanganu-Opatz, J. Simon Wiegert   +6 more
doaj   +1 more source

Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging. [PDF]

PLoS ONE, 2014
For a comprehensive understanding of cellular processes and potential dysfunctions therein, an analysis of the ubiquitous intracellular second messenger calcium is of particular interest.
Karolina Jahn, Carsten Hille
doaj   +1 more source

Calcium imaging for analgesic drug discovery

Neurobiology of Pain, 2022
Somatosensation and pain are complex phenomena involving a rangeofspecialised cell types forming different circuits within the peripheral and central nervous systems.
Federico Iseppon, John E. Linley, John N. Wood   +2 more
doaj   +1 more source

Calcium Imaging Studies in Enteric Glia. [PDF]

Methods Mol Biol
Calcium (Ca2+) imaging is a versatile technique to study cellular activity and is a particularly important tool to study electrically passive cells such as glia. Enteric glia play important roles in gut physiology, and their activity can be captured using Ca2+ imaging.
Thomasi B, Jamka J, Gulbransen BD.
europepmc   +3 more sources

A Novel Method to Quantify Calcium Response Pattern and Oscillation Using Fura2 and Acridine Orange

Journal of Pharmacological Sciences, 2004
To study calcium imaging data of cell populations that have various response patterns in peak amplitude and frequency of calcium oscillation in response to stimulation, comprehensive characterization based on statistical analysis of each response is ...
Mitsuhiro Morita, Jinichi Susuki, Takanori Moto, Chitose Higuchi, Yoshihisa Kudo   +4 more
doaj   +1 more source

Neuronal Graphs: A Graph Theory Primer for Microscopic, Functional Networks of Neurons Recorded by Calcium Imaging

Frontiers in Neural Circuits, 2021
Connected networks are a fundamental structure of neurobiology. Understanding these networks will help us elucidate the neural mechanisms of computation. Mathematically speaking these networks are “graphs”—structures containing objects that are connected.
Carl J. Nelson, Stephen Bonner
doaj   +1 more source