Results 31 to 40 of about 359,534 (258)

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

open access: yesNature Communications, 2021
Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden   +12 more
doaj   +1 more source

Calcium Imaging in Neuron Cell Death [PDF]

open access: yes, 2014
Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron cell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity. Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt ) in neurons concurrently with apoptosis and neuron ...
Calvo-Rodríguez, María   +2 more
openaire   +4 more sources

Dendritic spike saturation of endogenous calcium buffer and induction of postsynaptic cerebellar LTP. [PDF]

open access: yesPLoS ONE, 2008
The architecture of parallel fiber axons contacting cerebellar Purkinje neurons retains spatial information over long distances. Parallel fiber synapses can trigger local dendritic calcium spikes, but whether and how this calcium signal leads to plastic ...
Marco Canepari, Kaspar E Vogt
doaj   +1 more source

Calcium imaging in the optical stretcher

open access: yesOptics Express, 2011
The Microfluidic Optical Stretcher (MOS) has previously been shown to be a versatile tool to measure mechanical properties of single suspended cells. In this study we combine optical stretching and fluorescent calcium imaging. A cell line transfected with a heat sensitive cation channel was used as a model system to show the versatility of the setup ...
Markus, Gyger   +6 more
openaire   +2 more sources

FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks

open access: yesBrain Sciences
Calcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions.
Márton Péter, László Héja
doaj   +1 more source

IP3 mediated global Ca2+ signals arise through two temporally and spatially distinct modes of Ca2+ release

open access: yeseLife, 2020
The ‘building-block’ model of inositol trisphosphate (IP3)-mediated Ca2+ liberation posits that cell-wide cytosolic Ca2+ signals arise through coordinated activation of localized Ca2+ puffs generated by stationary clusters of IP3 receptors (IP3Rs). Here,
Jeffrey T Lock, Ian Parker
doaj   +1 more source

CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data

open access: yesBMC Neuroscience, 2022
Background Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced ...
Madhavi Tippani   +9 more
doaj   +1 more source

Hybrid volumetric calcium imaging [PDF]

open access: yesNature Methods, 2019
Hybrid multiplexed sculpted light microscopy enables calcium imaging of volumes as large as half a cubic millimeter.
openaire   +2 more sources

Emapalumab for Immune Effector Cell‐Associated Hemophagocytic Lymphohistiocytosis‐Like Syndrome Following CD19‐Directed CAR‐T in Two Patients With B‐ALL: Clinical and Biomarker Correlates

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Immune effector cell‐associated hemophagocytic lymphohistiocytosis‐like syndrome (IEC‐HS) is a life‐threatening hyperinflammatory toxicity distinct from cytokine release syndrome (CRS) and neurotoxicity following chimeric antigen receptor T‐cell (CAR‐T) therapy. In a single‐institution retrospective cohort of pediatric and young adult patients
Thomas J. Galletta   +6 more
wiley   +1 more source

Detection of multivessel calcific disease progression in a patient with chronic limb-threatening ischemia using fluorine-18 sodium fluoride positron emission tomography imaging

open access: yesJournal of Vascular Surgery Cases and Innovative Techniques, 2023
Vascular calcification contributes to morbidity and poor clinical outcomes for patients with peripheral artery disease; however, the traditional assessment of the calcium burden using computed tomography (CT) imaging or angiography represents already ...
Ting-Heng Chou, PhD   +5 more
doaj   +1 more source

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