Results 31 to 40 of about 362,233 (261)
Calcium imaging in the optical stretcher
Optics Express, 2011 The Microfluidic Optical Stretcher (MOS) has previously been shown to be a versatile tool to measure mechanical properties of single suspended cells. In this study we combine optical stretching and fluorescent calcium imaging. A cell line transfected with a heat sensitive cation channel was used as a model system to show the versatility of the setup ...
Markus, Gyger +6 more
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Brain SciencesCalcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions.
Márton Péter, László Héja
doaj +1 more source
A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
Nature Communications, 2021 Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden +12 more
doaj +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Cup‐like nuclei are a distinctive morphological feature observed in certain cases of acute lymphoblastic leukemia (ALL). We provide evidence that they characterize DUX4/ERG ALL independently of IKZF1 deletion and reveal marked mitochondrial accumulation in this ALL subset.
Chloé Arfeuille +9 more
wiley +1 more source
Hybrid volumetric calcium imaging [PDF]
Nature Methods, 2019 Hybrid multiplexed sculpted light microscopy enables calcium imaging of volumes as large as half a cubic millimeter.
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Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy.
PLoS ONE, 2015 Imaging intracellular calcium concentration via reporters that change their fluorescence properties upon binding of calcium, referred to as calcium imaging, has revolutionized our way to probe neuronal activity non-invasively.
Marcel A Lauterbach +4 more
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Pediatric Blood &Cancer, EarlyView.ABSTRACT Primary cutaneous anaplastic large cell lymphoma (pcALCL) is a rare pediatric CD30‐positive T‐cell lymphoproliferative disorder with an excellent prognosis, but its genomic drivers are poorly defined. We report three children with skin‐limited disease demonstrating striking molecular heterogeneity, including NPM::ALK, NUP214::FRK, and a novel ...
Shoshana Greenberger +7 more
wiley +1 more source
Laser induced calcium oscillations in fluorescent calcium imaging
General physiology and biophysics, 2018 Phototoxicity is the most common problem investigators may encounter when performing live cell imaging. It develops due to excess laser exposure of cells loaded with fluorophores and can lead to often overlooked but significant artifacts, such as massive increase of intracellular Ca2+ concentration, which would make data interpretation problematic ...
Vincze, János +7 more
openaire +4 more sources
Estimating firing rates from calcium signals in locust projection neurons in vivo
Frontiers in Neural Circuits, 2007 Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe.
Laurent C Moreaux, Gilles Laurent
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Pediatric Blood &Cancer, EarlyView.ABSTRACT Background Central nervous system (CNS) neuroblastoma, FOXR2‐activated, is a recently recognized entity in the WHO CNS5 classification, defined by activation of the FOXR2 transcription factor and unique histopathological features. This review synthesizes available literature and pooled clinical data, providing insight into demographics ...
Sudarshawn Damodharan +1 more
wiley +1 more source