Bio-Protocol, 2017 Fertilization calcium waves are a conserved trigger for animal development; however, genetic analysis of these waves has been limited due to the difficulty of imaging in vivo fertilization.
Jun Takayama +2 more
doaj +1 more source
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. [PDF]
, 2020 Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a
Barson, Daniel +7 more
core
Simultaneous whole-animal 3D-imaging of neuronal activity using light field microscopy [PDF]
, 2014 3D functional imaging of neuronal activity in entire organisms at single cell level and physiologically relevant time scales faces major obstacles due to trade-offs between the size of the imaged volumes, and spatial and temporal resolution.
A Cheng +35 more
core +3 more sources
High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators
iScience, 2019 Summary: Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical ...
Urs A.T. Hofmann +6 more
doaj +1 more source
Dendritic spike saturation of endogenous calcium buffer and induction of postsynaptic cerebellar LTP. [PDF]
PLoS ONE, 2008 The architecture of parallel fiber axons contacting cerebellar Purkinje neurons retains spatial information over long distances. Parallel fiber synapses can trigger local dendritic calcium spikes, but whether and how this calcium signal leads to plastic ...
Marco Canepari, Kaspar E Vogt
doaj +1 more source
Automatic cell segmentation by adaptive thresholding (ACSAT) for large-scale calcium imaging datasets [PDF]
, 2018 Advances in calcium imaging have made it possible to record from an increasingly larger number of neurons simultaneously. Neuroscientists can now routinely image hundreds to thousands of individual neurons.
Gritton, Howard J. +6 more
core +1 more source
Calcium Sensor for Photoacoustic Imaging
Journal of the American Chemical Society, 2017 We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction ...
Sheryl Roberts +17 more
openaire +5 more sources
Reading out a spatiotemporal population code by imaging neighbouring parallel fibre axons in vivo. [PDF]
, 2015 The spatiotemporal pattern of synaptic inputs to the dendritic tree is crucial for synaptic integration and plasticity. However, it is not known if input patterns driven by sensory stimuli are structured or random.
Häusser, M, Wilms, CD
core +1 more source
Focus ion beam/scanning electron microscopy characterization of osteoclastic resorption of calcium phosphate substrates [PDF]
, 2017 This article presents the application of dual focused ion beam/scanning electron microscopy (FIB-SEM) imaging for preclinical testing of calcium phosphates with osteoclast precursor cells and how this high-resolution imaging technique is able to reveal ...
Baldini, Nicola +6 more
core +2 more sources
Laser induced calcium oscillations in fluorescent calcium imaging
General physiology and biophysics, 2018 Phototoxicity is the most common problem investigators may encounter when performing live cell imaging. It develops due to excess laser exposure of cells loaded with fluorophores and can lead to often overlooked but significant artifacts, such as massive increase of intracellular Ca2+ concentration, which would make data interpretation problematic ...
Vincze, János +7 more
openaire +4 more sources