Results 51 to 60 of about 359,534 (258)

Correlative multiphoton-STED microscopy of podocyte calcium levels and slit diaphragm ultrastructure in the renal glomerulus

Scientific Reports
In recent years functional multiphoton (MP) imaging of vital mouse tissues and stimulation emission depletion (STED) imaging of optically cleared tissues allowed new insights into kidney biology.
Eva Wiesner, Julia Binz-Lotter, Agnes Hackl, David Unnersjö-Jess, Nelli Rutkowski, Thomas Benzing, Matthias J. Hackl   +6 more
doaj   +1 more source

Computational Methods for Neuron Segmentation in Two-Photon Calcium Imaging Data: A Survey

Applied Sciences, 2022
Calcium imaging has rapidly become a methodology of choice for real-time in vivo neuron analysis. Its application to large sets of data requires automated tools to annotate and segment cells, allowing scalable image segmentation under reproducible ...
Waseem Abbas, David Masip
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux

Cell Reports, 2015
Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging.
Rebecca W. Beerman, Molly A. Matty, Gina G. Au, Loren L. Looger, Kingshuk Roy Choudhury, Philipp J. Keller, David M. Tobin   +6 more
doaj   +1 more source

Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator

Frontiers in Cell and Developmental Biology, 2022
Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light ...
Mikhail E. Matlashov, Jorge Vera, Ludmila A. Kasatkina, Kamran Khodakhah, Vladislav V. Verkhusha, Vladislav V. Verkhusha   +5 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Calcium Imaging Analysis of Cellular Responses to Hypercapnia and Hypoxia in the NTS of Newborn Rat Brainstem Preparation

Frontiers in Physiology, 2021
It is supposed that the nucleus of the solitary tract (NTS) in the dorsal medulla includes gas sensor cells responsive to hypercapnia or hypoxia in the central nervous system.
Hiroshi Onimaru, Itaru Yazawa, Kotaro Takeda, Isato Fukushi, Isato Fukushi, Yasumasa Okada   +5 more
doaj   +1 more source

Calcium imaging in a FlAsH [PDF]

Nature Methods, 2007
A new calcium indicator called Calcium Green FlAsH allows researchers to monitor rapid and localized calcium dynamics in the cell.
openaire   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Improvements in Simultaneous Sodium and Calcium Imaging [PDF]

Frontiers in Cellular Neuroscience, 2019
High speed imaging of ion concentration changes in neurons is an important and growing tool for neuroscientists. We previously developed a system for simultaneously measuring sodium and calcium changes in small compartments in neurons (Miyazaki and Ross, 2015).
Kenichi Miyazaki, Kenichi Miyazaki, John E. Lisman, John E. Lisman, William N. Ross, William N. Ross   +5 more
openaire   +4 more sources