Results 51 to 60 of about 2,798,160 (306)
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
Laser induced calcium oscillations in fluorescent calcium imaging
General physiology and biophysics, 2018 Phototoxicity is the most common problem investigators may encounter when performing live cell imaging. It develops due to excess laser exposure of cells loaded with fluorophores and can lead to often overlooked but significant artifacts, such as massive increase of intracellular Ca2+ concentration, which would make data interpretation problematic ...
Vincze, János +7 more
openaire +4 more sources
Brain SciencesCalcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions.
Márton Péter, László Héja
doaj +1 more source
Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy.
PLoS ONE, 2015 Imaging intracellular calcium concentration via reporters that change their fluorescence properties upon binding of calcium, referred to as calcium imaging, has revolutionized our way to probe neuronal activity non-invasively.
Marcel A Lauterbach +4 more
doaj +1 more source
A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
Nature Communications, 2021 Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden +12 more
doaj +1 more source
FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source
Imaging single-channel calcium microdomains [PDF]
Cell Calcium, 2006 The Ca(2+) microdomains generated around the mouth of open ion channels represent the basic building blocks from which cytosolic Ca(2+) signals are constructed. Recent improvements in optical imaging techniques now allow these microdomains to be visualized as single channel calcium fluorescence transients (SCCaFTs), providing information about channel ...
Angelo, Demuro, Ian, Parker
openaire +2 more sources
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Calcium imaging analysis tools
, 2022 Matlab scripts to analyze calcium imaging data generated by the Inscopix. See DEADME.txt for details. The OASIS Matlab deconvolution package is needed. The programs also align imaging data with EEG data generated by the Neuralynx system. EEG2022Dist (DIO:
Yueqing Peng
core +1 more source
Estimating firing rates from calcium signals in locust projection neurons in vivo
Frontiers in Neural Circuits, 2007 Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe.
Laurent C Moreaux, Gilles Laurent
doaj +1 more source