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Calcium Imaging in Neuron Cell Death [PDF]
, 2014 Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron cell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity. Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt ) in neurons concurrently with apoptosis and neuron ...
Calvo-Rodríguez, María +2 more
openaire +4 more sources
A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium
Nature Communications, 2021 Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden +12 more
doaj +1 more source
, 2014 The recent development of genetically encoded calcium indicators enables monitoring in vivo the activity of neuronal populations. Most analysis of these calcium transients relies on linear regression analysis based on the sensory stimulus applied or the ...
Chavez, Mario +4 more
core +3 more sources
Brain SciencesCalcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions.
Márton Péter, László Héja
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Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy.
PLoS ONE, 2015 Imaging intracellular calcium concentration via reporters that change their fluorescence properties upon binding of calcium, referred to as calcium imaging, has revolutionized our way to probe neuronal activity non-invasively.
Marcel A Lauterbach +4 more
doaj +1 more source
Estimating firing rates from calcium signals in locust projection neurons in vivo
Frontiers in Neural Circuits, 2007 Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe.
Laurent C Moreaux, Gilles Laurent
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eLife, 2020 The ‘building-block’ model of inositol trisphosphate (IP3)-mediated Ca2+ liberation posits that cell-wide cytosolic Ca2+ signals arise through coordinated activation of localized Ca2+ puffs generated by stationary clusters of IP3 receptors (IP3Rs). Here,
Jeffrey T Lock, Ian Parker
doaj +1 more source
CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data
BMC Neuroscience, 2022 Background Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced ...
Madhavi Tippani +9 more
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Journal of Vascular Surgery Cases and Innovative Techniques, 2023 Vascular calcification contributes to morbidity and poor clinical outcomes for patients with peripheral artery disease; however, the traditional assessment of the calcium burden using computed tomography (CT) imaging or angiography represents already ...
Ting-Heng Chou, PhD +5 more
doaj +1 more source
Continuous volumetric imaging via an optical phase-locked ultrasound lens
, 2016 In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning ...
Cui, M. +7 more
core +1 more source