Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging. [PDF]
PLoS ONE, 2014 For a comprehensive understanding of cellular processes and potential dysfunctions therein, an analysis of the ubiquitous intracellular second messenger calcium is of particular interest.
Karolina Jahn, Carsten Hille
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FM1-43 reveals membrane recycling in adult inner hair cells of the mammalian cochlea [PDF]
, 2002 Neural transmission of complex sounds demands fast and sustained rates of synaptic release from the primary cochlear receptors, the inner hair cells (IHCs). The cells therefore require efficient membrane recycling.
Ashmore, JF +2 more
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Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging [PDF]
, 2007 We report a novel wavelength-flexible laser source for three-dimensional ultra-violet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband laser source extended from A = 331 nm into the visible region of the ...
Esposito, E., Harris, J., McConnell, G.
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Nature Neuroscience, 2020 Inference of action potentials (‘spikes’) from neuronal calcium signals is complicated by the scarcity of simultaneous measurements of action potentials and calcium signals (‘ground truth’).
Peter Rupprecht +10 more
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Multiphoton Intravital Calcium Imaging [PDF]
Current Protocols in Cytometry, 2018 AbstractMultiphoton intravital calcium imaging is a powerful technique that enables high‐resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2‐photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain.
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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis [PDF]
, 2020 Spontaneous intracellular calcium activity can be observed in a variety of cell types and is proposed to play critical roles in a variety of physiological processes.
Ablondi, Eileen F. +7 more
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Automatic cell segmentation by adaptive thresholding (ACSAT) for large-scale calcium imaging datasets [PDF]
, 2018 Advances in calcium imaging have made it possible to record from an increasingly larger number of neurons simultaneously. Neuroscientists can now routinely image hundreds to thousands of individual neurons.
Gritton, Howard J. +6 more
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Hybrid volumetric calcium imaging [PDF]
Nature Methods, 2019 Hybrid multiplexed sculpted light microscopy enables calcium imaging of volumes as large as half a cubic millimeter.
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In-vivo two-photon imaging of the honey bee antennal lobe [PDF]
, 2010 Due to the honey bee's importance as a simple neural model, there is a great need for new functional imaging modalities. Herein we report on the use of two-photon microscopy for in-vivo functional and morphological imaging of the honey bee's olfactory ...
Anfora, Gianfranco +6 more
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Dysfunction of cortical GABAergic neurons leads to sensory hyper-reactivity in a Shank3 mouse model of ASD. [PDF]
, 2020 Hyper-reactivity to sensory input is a common and debilitating symptom in individuals with autism spectrum disorders (ASD), but the neural basis underlying sensory abnormality is not completely understood.
Chen, Naiyan +16 more
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