Genome-wide profiling of human cap-independent translation-enhancing elements. [PDF]
Nat Methods, 2013 We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing.
Wellensiek BP +10 more
europepmc +8 more sources
A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation [PDF]
Scientific Reports, 2021 Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE ...
Brandon M. Trainor +4 more
doaj +2 more sources
Cap-Independent mRNA Translation in Germ Cells [PDF]
International Journal of Molecular Sciences, 2019 Cellular mRNAs in plants and animals have a 5′-cap structure that is accepted as the recognition point to initiate translation by ribosomes. Consequently, it was long assumed that the translation initiation apparatus was built solely for a cap-dependent (CD) mechanism.
Keiper, Brett D., Brett D. Keiper
core +5 more sources
Mapping of sequences in the 5' region and 3' UTR of tomato ringspot virus RNA2 that facilitate cap-independent translation of reporter transcripts in vitro. [PDF]
PLoS ONE, 2021 Tomato ringspot virus (ToRSV, genus Nepovirus, family Secoviridae, order Picornavirales) is a bipartite positive-strand RNA virus, with each RNA encoding one large polyprotein. ToRSV RNAs are linked to a 5'-viral genome-linked protein (VPg) and have a 3'
Dinesh Babu Paudel, Hélène Sanfaçon
doaj +2 more sources
The Cap-Independent Translation of Survivin 5′UTR and HIV-1 IRES Sequences Is Inhibited by Oxidative Stress Produced by H. pylori Gamma-Glutamyl Transpeptidase Activity [PDF]
BiomoleculesBackground: Survivin is an anti-apoptotic protein highly expressed during embryonic development and, in adults, mainly in the gastrointestinal epithelium. Its levels decrease in human gastric tissue and cultured cells upon exposure to Helicobacter pylori
Mariaignacia Rubilar +9 more
doaj +2 more sources
A Dual Interaction Between the 5′- and 3′-Ends of the Melon Necrotic Spot Virus (MNSV) RNA Genome Is Required for Efficient Cap-Independent Translation [PDF]
Frontiers in Plant Science, 2018 In eukaryotes, the formation of a 5′-cap and 3′-poly(A) dependent protein–protein bridge is required for translation of its mRNAs. In contrast, several plant virus RNA genomes lack both of these mRNA features, but instead have a 3′-CITE (for cap ...
Manuel Miras +7 more
doaj +2 more sources
DDX3 Regulates the Cap‐Independent Translation of the Japanese Encephalitis Virus via Its Interactions with PABP1 and the Untranslated Regions of the Viral Genome [PDF]
Advanced ScienceThe translation of global cellular proteins is almost completely repressed in cells with flavivirus infection, while viral translation remains efficient. The mechanisms of flaviviruses evade host translational shutoff are largely unknown.
Chenxi Li +13 more
doaj +2 more sources
Cap-Independent Translational Control of Carcinogenesis [PDF]
Frontiers in Oncology, 2016 Translational regulation has been shown to play an important role in cancer and tumor progression. Despite this fact, the role of translational control in cancer is an understudied and under appreciated field, most likely due to the technological hurdles
Beth eWalters, Sunnie R. Thompson
doaj +3 more sources
Internal Ribosome Entry Sites Mediate Cap-Independent Translation of Bmi1 in Nasopharyngeal Carcinoma [PDF]
Frontiers in Oncology, 2020 Bmi1 is overexpressed in multiple human cancers. We previously reported the oncogenic function and the transcription regulation mechanisms of Bmi1 in nasopharyngeal carcinoma (NPC).
Hongbo Wang +16 more
doaj +2 more sources
3' cap-independent translation enhancers of plant viruses. [PDF]
Annu Rev Microbiol, 2013 In the absence of a 5′ cap, plant positive-strand RNA viruses have evolved a number of different elements in their 3′ untranslated region (UTR) to attract initiation factors and/or ribosomes to their templates. These 3′ cap-independent translational enhancers (3′ CITEs) take different forms, such as I-shaped, Y-shaped, T-shaped, or pseudoknotted ...
Simon AE, Miller WA.
europepmc +4 more sources