Results 11 to 20 of about 4,965,597 (147)

Systematic multi-trait AAV capsid engineering for efficient gene delivery [PDF]

Nature Communications, 2022
Broadening gene therapy applications requires manufacturable vectors that efficiently transduce target cells in humans and preclinical models. Conventional selections of adeno-associated virus (AAV) capsid libraries are inefficient at searching the vast ...
Fatma-Elzahraa Eid, Albert T. Chen, Ken Y. Chan, Qin Huang, Qingxia Zheng, Isabelle G. Tobey, Simon Pacouret, Pamela P. Brauer, Casey Keyes, Megan Powell, Jencilin Johnston, Binhui Zhao, Kasper Lage, Alice F. Tarantal, Yujia A. Chan, Benjamin E. Deverman   +15 more
doaj   +6 more sources

Engineering the AAV capsid to evade immune responses [PDF]

Current Opinion in Biotechnology, 2019
Gene therapy is progressively emerging as a promising and powerful therapeutic modality, and adeno-associated virus (AAV) is a major delivery vehicle for such therapies. Among the most significant challenges that limit AAV's utility, however, is the immune response it elicits. Antibodies elicited by prior exposure to natural virus or vector can bind to
Barnes, Christopher, Scheideler, Olivia, Schaffer, David   +2 more
openaire   +6 more sources

In Vivo Capsid Engineering of Bacteriophages for Oriented Surface Conjugation

ACS Applied Bio Materials, 2022
The current state-of-the-art in bacteriophage (phage) immobilization onto magnetic particles is limited to techniques that are less expensive and/or facile but nonspecific or those that are more expensive and/or complicated but ensure capsid-down orientation of the phages, as necessary to preserve infectivity and performance in subsequent applications (
Kathryn A. Hufziger, Emma L. Farquharson, Brenda G. Werner, Qingmin Chen, Julie M. Goddard, Sam R. Nugen   +5 more
openaire   +3 more sources

Structure-based engineering of papillomavirus major capsid L1: controlling particle assembly [PDF]

Virology Journal, 2007
The outer shell of the papillomavirus particle is comprised of 72 pentamers of the major capsid L1 protein arranged on a T = 7 icosahedral lattice. The recombinant L1 can form T = 7 virus-like particles in vitro.
Dasgupta Jhimli, Bishop Brooke, Chen Xiaojiang S   +2 more
doaj   +3 more sources

Expanding Adeno-Associated Viral Capsid Engineering to Multiple Variable Regions for Diversified Tropism [PDF]

, 2023
Adeno-associated virus research is critical for the advancement of gene therapy and treatment of myriad debilitating genetic disorders. Targeted delivery of genetic components to a tissue or cell population remains a bottleneck for gene therapy, but the ...
Goertsen, David Gerald
core   +1 more source

Engineered Capsids for Efficient Gene Delivery to the Retina and Cornea [PDF]

Human Gene Therapy, 2020
Adeno-associated viral (AAV) vectors represent an ideal vehicle for human gene transfer. One advantage to the AAV vector system is the availability of multiple naturally occurring serotypes that provide selective tropisms for various target cells. Strategies to enhance the properties of the natural AAV isolates have been developed and can be divided ...
Amy, Frederick, Jennifer, Sullivan, Lin, Liu, Matthew, Adamowicz, Michael, Lukason, Jasmine, Raymer, Zhengyu, Luo, Xiaoying, Jin, Kollu Nageswara, Rao, Catherine, O'Riordan   +9 more
openaire   +2 more sources

Engineering the PP7 Virus Capsid as a Peptide Display Platform [PDF]

ACS Nano, 2019
As self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the genetic modification and structural characterization of the Leviviridae PP7 capsid protein as a platform for the presentation of functional polypeptides.
Liangjun Zhao, Mykhailo Kopylov, Clinton S. Potter, Bridget Carragher, M. G. Finn   +4 more
openaire   +2 more sources

Arthritogenic Alphavirus Capsid Protein

, 2021
In the past two decades Old World and arthritogenic alphavirus have been responsible for epidemics of polyarthritis, causing high morbidity and becoming a major public health concern. The multifunctional arthritogenic alphavirus capsid protein is crucial
Taylor, Adam, Shambhavi Rao, Adam Taylor, Rao, Shambhavi   +3 more
core   +1 more source

Binding of external ligands onto an engineered virus capsid [PDF]

Protein Engineering, Design and Selection, 2001
The development of novel delivery systems for therapeutic substances includes targeting of the carriers to a specific site or tissue within the body of the recipient. This can be accomplished by appropriate receptor-binding domains and requires linking of these domains to the carrier.
U, Schmidt, R, Rudolph, G, Böhm
openaire   +2 more sources

A capsid-encoded PPxY-motif facilitates adenovirus entry. [PDF]

, 2010
Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and ...
Segura-Morales, Carolina, Ruzsics, Zsolt, Kremer, Eric J, Wiethoff, Christopher M, Kremer Eric J., Baptist Jammart, Wodrich, Harald, Jammart, Baptist, Seelmeir Sigrid, Jammart Baptist, Christopher M Wiethoff, Wiethoff, Christopher M., Ruzsics Zsolt, Segura-Morales Carolina, Seelmeir, Sigrid, Carolina Segura-Morales, Henaff, Daniel, Zsolt Ruzsics, Kremer, Eric J., Coux, Olivier, Harald Wodrich, Henaff Daniel, Sigrid Seelmeir, Coux Olivier, Wodrich Harald, Eric J Kremer, Daniel Henaff, Olivier Coux, Wiethoff Christopher M.   +28 more
core   +1 more source