Results 11 to 20 of about 31,732 (250)
Scratching the surface of RGD-directed AAV capsid engineering. [PDF]
Mol Ther, 2021 Zolotukhin S +3 more
europepmc +3 more sources
In Vivo Capsid Engineering of Bacteriophages for Oriented Surface Conjugation. [PDF]
ACS Appl Bio Mater, 2022 The current state-of-the-art in bacteriophage (phage) immobilization onto magnetic particles is limited to techniques that are less expensive and/or facile but nonspecific or those that are more expensive and/or complicated but ensure capsid-down orientation of the phages, as necessary to preserve infectivity and performance in subsequent applications (
Hufziger KA +5 more
europepmc +3 more sources
Engineered Capsids for Efficient Gene Delivery to the Retina and Cornea [PDF]
Human Gene Therapy, 2020 Adeno-associated viral (AAV) vectors represent an ideal vehicle for human gene transfer. One advantage to the AAV vector system is the availability of multiple naturally occurring serotypes that provide selective tropisms for various target cells. Strategies to enhance the properties of the natural AAV isolates have been developed and can be divided ...
Amy, Frederick +9 more
openaire +2 more sources
Engineering the AAV capsid to evade immune responses [PDF]
Current Opinion in Biotechnology, 2019 Gene therapy is progressively emerging as a promising and powerful therapeutic modality, and adeno-associated virus (AAV) is a major delivery vehicle for such therapies. Among the most significant challenges that limit AAV's utility, however, is the immune response it elicits. Antibodies elicited by prior exposure to natural virus or vector can bind to
Barnes, Christopher +2 more
openaire +4 more sources
Pathogens, 2022 Parvoviruses are a diverse family of small, non-enveloped DNA viruses that infect a wide variety of species, tissues and cell types. For over half a century, their intriguing biology and pathophysiology has fueled intensive research aimed at dissecting ...
Jonas Becker, Julia Fakhiri, Dirk Grimm
doaj +1 more source
A versatile toolkit for overcoming AAV immunity
Frontiers in Immunology, 2022 Recombinant adeno-associated virus (AAV) is a promising delivery vehicle for in vivo gene therapy and has been widely used in >200 clinical trials globally.
Xuefeng Li +5 more
doaj +1 more source
Engineering the PP7 Virus Capsid as a Peptide Display Platform [PDF]
ACS Nano, 2019 As self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the genetic modification and structural characterization of the Leviviridae PP7 capsid protein as a platform for the presentation of functional polypeptides.
Liangjun Zhao +4 more
openaire +2 more sources
Binding of external ligands onto an engineered virus capsid [PDF]
Protein Engineering, Design and Selection, 2001 The development of novel delivery systems for therapeutic substances includes targeting of the carriers to a specific site or tissue within the body of the recipient. This can be accomplished by appropriate receptor-binding domains and requires linking of these domains to the carrier.
U, Schmidt, R, Rudolph, G, Böhm
openaire +2 more sources
Structural Insights into the Human Astrovirus Capsid
Viruses, 2021 Astroviruses (AstVs) are non-enveloped, positive single-stranded RNA viruses that cause a wide range of inflammatory diseases in mammalian and avian hosts.
Matthew Ykema, Yizhi J. Tao
doaj +1 more source
Structural basis of receptor usage by the engineered capsid AAV-PHP.eB
Molecular Therapy - Methods & Clinical Development, 2022 Adeno-associated virus serotype 9 (AAV9) is a promising gene therapy vector for treating neurodegenerative diseases due to its ability to penetrate the blood-brain barrier. PHP.eB was engineered from AAV9 by insertion of a 7-amino acid peptide and point mutation of neighboring residues, thereby enhancing potency in the central nervous system.
Seongmin Jang +4 more
openaire +5 more sources