Results 121 to 130 of about 6,660,257 (317)

Machine Learning‐Enhanced Random Matrix Theory Design for Human Immunodeficiency Virus Vaccine Development

Advanced Intelligent Discovery, EarlyView.
This study integrates random matrix theory (RMT) and principal component analysis (PCA) to improve the identification of correlated regions in HIV protein sequences for vaccine design. PCA validation enhances the reliability of RMT‐derived correlations, particularly in small‐sample, high‐dimensional datasets, enabling more accurate detection of ...
Mariyam Siddiqah, Muhammad Zulqarnain Zeb, Muhammad Husnain Zeb, Babar Shabbir   +3 more
wiley   +1 more source

Protein chainmail: catenated protein in viral capsids.

Cell, 1998
The capsid shells of bacteriophage HK97 and several other phages contain polypeptides that are covalently linked into complexes so large that they do not enter polyacrylamide gels after denaturation. The enormous apparent size of these protein complexes in HK97 derives from a novel protein topology.
openaire   +2 more sources

Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody

Animal Research and One Health, EarlyView.
This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng, Xuyan Xiang, Xinyu Qi, Hanrong Zhou, Peiyu Xiao, Fandan Meng, Tongqing An, Haiwei Wang   +7 more
wiley   +1 more source

Near-atomic structure of a giant virus

Nature Communications, 2019
Nucleocytoplasmic large DNA viruses are a group of viruses that infect many eukaryotic hosts. Here, the authors provide a 3.5 Å resolution icosahedrally-averaged capsid structure of Paramecium bursaria chlorella virus 1 and show how 1800 minor capsid ...
Qianglin Fang, Dongjie Zhu, Irina Agarkova, Jagat Adhikari, Thomas Klose, Yue Liu, Zhenguo Chen, Yingyuan Sun, Michael L. Gross, James L. Van Etten, Xinzheng Zhang, Michael G. Rossmann   +11 more
doaj   +1 more source

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

Transportin 3 promotes a nuclear maturation step required for efficient HIV-1 integration.

PLoS Pathogens, 2011
The HIV/AIDS pandemic is a major global health threat and understanding the detailed molecular mechanisms of HIV replication is critical for the development of novel therapeutics.
Lihong Zhou, Elena Sokolskaja, Clare Jolly, William James, Sally A Cowley, Ariberto Fassati   +5 more
doaj   +1 more source

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Oral Squamous Cell Carcinoma‐Risk Factors, Diagnosis, Treatment, and Prevention

Cancer Nexus, EarlyView.
ABSTRACT Oral squamous cell carcinoma (OSCC) usually originates from the precancerous lesions of oral mucosa and accounts for approximately 90% of oral cancers. In‐depth understanding of pathogenesis, hallmarks, and etiological factors is a crucial prerequisite for advancing the diagnosis and treatment for OSCC patients. This review aims to present the
Liping Wang, Xiaole Liu, Mengjie Chen, Ying Hu, Lilei Zhu   +4 more
wiley   +1 more source

Site-Specific N-Glycosylation on the AAV8 Capsid Protein

Viruses, 2018
Adeno associated virus (AAV) is a versatile gene delivery tool, which has been approved as a human gene therapy vector for combating genetic diseases. AAV capsid proteins are the major components that determine the tissue specificity, immunogenicity and ...
Arya Aloor, Junping Zhang, Ebtesam A. Gashash, Aishwarya Parameswaran, Matthew Chrzanowski, Cheng Ma, Yong Diao, Peng George Wang, Weidong Xiao   +8 more
doaj   +1 more source

Capsid protein heterogeneity in turnip mosaic virus

Virology, 1976
Abstract The effect of capsid protein heterogeneity on various virus properties in purified turnip mosaic virus was investigated. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate (SDS) revealed three distinct capsid protein species, designated as fast form (MW 27,000), intermediate form (MW 29,000), slow form (MW 36,000 ...
Plant Pathology Department, University of Florida, Gainesville, Florida 32611 USA ( host institution ), Hiebert, Ernest, McDonald, J.G.   +2 more
openaire   +3 more sources