Results 121 to 130 of about 5,586,089 (360)
Animal Research and One Health, EarlyView.Rabies Transmission and Control in the Somali Region. Introduction: Rabies is a fatal zoonotic disease caused by the Lyssavirus, posing a significant threat to both wildlife and public health in the Somali Region of Ethiopia. Key Points: 1. Primary Hosts: Wild carnivores such as foxes, jackals, raccoons, skunks, bats, and honey badgers are the main ...
Hassan Abdi Hussein
wiley +1 more source
Electrostatics and the Assembly of an RNA Virus [PDF]
, 2004 Electrostatic interactions play a central role in the assembly of single-stranded RNA viruses. Under physiological conditions of salinity and acidity, virus capsid assembly requires the presence of genomic material that is oppositely charged to the core proteins. In this paper we apply basic polymer physics and statistical mechanics methods to the self-
arxiv +1 more source
Site-Specific N-Glycosylation on the AAV8 Capsid Protein
Viruses, 2018 Adeno associated virus (AAV) is a versatile gene delivery tool, which has been approved as a human gene therapy vector for combating genetic diseases. AAV capsid proteins are the major components that determine the tissue specificity, immunogenicity and ...
Arya Aloor +8 more
doaj +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green +9 more
wiley +1 more source
Combinatorial rules of icosahedral capsid growth [PDF]
arXiv, 2005 A model of growth of icosahedral viral capsids is proposed. It takes into account the diversity of hexamers' compositions, leading to definite capsid size. We show that the observed yield of capsid production implies a very high level of self-organization of elementary building blocks.
arxiv
Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification
Biotechnology and Bioengineering, EarlyView.Peptide‐functionalized affinity resins with high adenovirus binding capacity, recovery, and contaminant removal afford excellent productivity, and reduced impurities in a three‐step purification process. ABSTRACT Adenoviral vectors (AdVs) are gaining prominence in cancer therapy and vaccine development, posing the need for a modern AdV manufacturing ...
Yuxuan Wu +7 more
wiley +1 more source
Mechanisms of virus assembly [PDF]
, 2014 Viruses are nanoscale entities containing a nucleic acid genome encased in a protein shell called a capsid, and in some cases surrounded by a lipid bilayer membrane. This review summarizes the physics that govern the processes by which capsids assembles within their host cells and in vitro.
arxiv +1 more source
Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production
Biotechnology and Bioengineering, EarlyView.ABSTRACT Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this
Juan J. Romero +6 more
wiley +1 more source
Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome [PDF]
, 2018 The formation of a viral capsid -- the highly-ordered protein shell that surrounds the genome of a virus -- is the canonical example of self-assembly. The capsids of many positive-sense RNA viruses spontaneously assemble from in vitro mixtures of the coat protein and RNA.
arxiv +1 more source
Biotechnology Progress, EarlyView.Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd +2 more
wiley +1 more source