Results 131 to 140 of about 5,586,089 (360)

Iterative hybrid model based optimization of rAAV production

Biotechnology Progress, EarlyView.
Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller, Gerald Siegwart, Susanne Heider, Michael Sokolov, Angela Botros, Alexandra Umprecht, Moritz von Stosch, Mariano Nicolas Cruz Bournazou   +7 more
wiley   +1 more source

Epitopes Expressed in Different Adenovirus Capsid Proteins Induce Different Levels of Epitope-Specific Immunity

Journal of Virology, 2005
On the basis of the concept that the capsid proteins of adenovirus (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with ...
Anja Krause, Ju Joh, N. Hackett, P. Roelvink, J. Bruder, T. Wickham, I. Kovesdi, R. Crystal, S. Worgall   +8 more
semanticscholar   +1 more source

A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is one of the most widely used viral vectors for gene therapy. It is used in very high doses for the treatment of many diseases, making large‐scale production for clinical applications challenging. We have established a synthetic biology‐based platform to construct stable production cell lines, which ...
Yu‐Chieh Lin, Han‐Jung Kuo, Min Lu, Thomas Mahl, George Aslanidi, Wei‐Shou Hu   +5 more
wiley   +1 more source

Epitope mapping of Grapevine virus A capsid protein

Archives of Virology, 2002
Previously characterized monoclonal antibodies (MAbs) to Grapevine virus A (GVA) showed a differential reactivity against intact or partially destabilized virus particles [2]. In the present study, this differential reactivity was confirmed and several peptides reacting with a panel of four different antibodies were identified by the PEPSCAN method of ...
M Dell'Orco, P Saldarelli, A Minafra, D Boscia, D Gallitelli   +4 more
openaire   +4 more sources

Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry.

Proceedings of the National Academy of Sciences of the United States of America, 2005
Gene product (gp) 24 of bacteriophage T4 forms the pentameric vertices of the capsid. Using x-ray crystallography, we found the principal domain of gp24 to have a polypeptide fold similar to that of the HK97 phage capsid protein plus an additional ...
A. Fokine, P. Leiman, M. Shneider, B. Ahvazi, Karen M. Boeshans, A. Steven, L. Black, V. Mesyanzhinov, M. Rossmann   +8 more
semanticscholar   +1 more source

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293‐based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper ...
Han‐Jung Kuo, Prahalad Srinivasan, Yu‐Chieh Lin, Min Lu, Carissa Rungkittikhun, Qi Zhang, Wei‐Shou Hu   +6 more
wiley   +1 more source

Nucleolar localization of potato leafroll virus capsid proteins.

Journal of General Virology, 2005
Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene.
S. Haupt, T. Stroganova, E. Ryabov, Sang Hyon Kim, G. Fraser, G. Duncan, M. Mayo, H. Barker, M. Taliansky   +8 more
semanticscholar   +1 more source

Promising Prodiginins Biological Activities

Chemistry &Biodiversity, EarlyView.
ABSTRACT Prodiginins are a large family of at least 34 pyrrolic compounds, including the well‐studied red pigment prodigiosin. Prodiginins are produced by several microorganisms displaying broad biological activities, including antimicrobial, antiviral, antiparasitic, antiproliferative, and immunosuppressive activities.
María F. Ladetto, Melisa E. Gantner, Boris E. Rodenak‐Kladniew, Santiago Rodriguez, María L. Cuestas, Alan Talevi, Guillermo R. Castro   +6 more
wiley   +1 more source

First Phosphonic‐Type Inhibitors and Activity‐Based Probes Specific to the O'nyong‐nyong Virus Capsid Protease

ChemMedChem, EarlyView.
The serine capsid protease (CP) catalyzes a crucial process of the alphaviral replication cycle; therefore, it constitutes an interesting therapy target. Herein, the O'nyong‐nyong virus CP profile activity with a specific fluorogenic substrate is analyzed and examines the inhibitory activity of a new series of phosphonic analogs of tryptophan and ...
Karolina Torzyk‐Jurowska, Agnieszka Łupicka‐Słowik, Renata Grzywa, Yuliya Chykunova, Krzysztof Pyrć, Marcin Sieńczyk   +5 more
wiley   +1 more source

Using computational approaches to study dengue virus capsid assembly

Computational and Mathematical Biophysics, 2019
Dengue viral capsid plays a significant role in viral life cycle of dengue, especially in vial genome protection and virus-cell fusion. Revealing mechanisms of the viral capsid protein assembly may lead to the discovery of anti-viral drugs that inhibit ...
Salas Gicela G Saucedo, Hernandez Alan E Lopez, He Jiadi, Karki Chitra, Xie Yixin, Sun Shengjie, Xian Yuejiao, Li Lin   +7 more
doaj   +1 more source