Results 51 to 60 of about 129,911 (267)

Structural characterization of the carbohydrate-binding module of NanA sialidase, a pneumococcal virulence factor

, 2015
This research was supported by the University of St Andrews and grants provided by the Medical Research Council.Background: Streptococcus pneumoniae Neuraminidase A (NanA) is a multi-domain protein anchored to the bacterial surface.
Helen Connaris, Yang, Lei, Garry L. Taylor, Taylor, Garry Lindsay, Lei Yang, Potter, Jane Alexandra, Connaris, Helen, Jane A. Potter   +7 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Characterization of strain degeneration in the RL-P37 strain lineage of Trichoderma reesei

Biotechnology for Biofuels and Bioproducts
Spontaneous strain degeneration, defined as the loss of an essential biological function during prolonged usage, is frequently observed in microorganisms and poses a significant challenge to the biotechnology industry.
Caroline Danner, Armin Gabriel, Christian Zimmermann, Robert L. Mach, Yuriy Karpenko, Igor Nikolaev, Sharief Barends, Astrid R. Mach-Aigner   +7 more
doaj   +1 more source

Systems approaches to predict the functions of glycoside hydrolases during the life cycle of Aspergillus niger using developmental mutants ∆brlA and ∆flbA.

PLoS ONE, 2015
BackgroundThe filamentous fungus Aspergillus niger encounters carbon starvation in nature as well as during industrial fermentations. In response, regulatory networks initiate and control autolysis and sporulation.
Jolanda M van Munster, Benjamin M Nitsche, Michiel Akeroyd, Lubbert Dijkhuizen, Marc J E C van der Maarel, Arthur F J Ram   +5 more
doaj   +1 more source

Metatranscriptomic holobiont analysis of carbohydrate-active enzymes in the millipede Telodeinopus aoutii (Diplopoda, Spirostreptida)

Frontiers in Ecology and Evolution, 2022
As important decomposers of soil organic matter, millipedes contribute to lignocellulose decomposition and nutrient cycling. The degradation of lignocellulose requires the action of several carbohydrate-active enzymes (CAZymes) and, in most invertebrates,
Puspendu Sardar, Vladimír Šustr, Alica Chroňáková, František Lorenc, František Lorenc   +4 more
doaj   +1 more source

The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site [PDF]

, 1998
Background: The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) catalyses the cellular uptake and subsequent phosphorylation of carbohydrates.
van Montfort, R.L M, Dijkstra, B.W., Dijkstra, Bauke W., Robillard, G.T., Kalk, Kor H., Kouwijzer, Milou L.C.E.,, Pijning, Tjaard, van Montfort, Rob LM, van Montfort, Robert, Montfort, Rob L.M. van,, Dijkstra, Bauke W.,, Robillard, George T.,, Kalk, Kor H, Pijning, T., Kouwijzer, Milou LCE, Hanqyi, I., Robillard, George T, Dijkstra, Bauke W, Kalk, K.H., Pijning, Tjaard,, Pijning, Tjaard; id_orcid, Kouwijzer, Marie, Kalk, Kor H.,, Hangyi, Ilona,, Robillard, George T., Hangyi, Ilona, Kouwijzer, M.L C E   +26 more
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Structural and functional properties of pectin and lignin–carbohydrate complexes de-esterases: a review

Bioresources and Bioprocessing, 2018
Biological conversion of plant biomass into commercially valuable products is one of the highly studied subjects in the last two decades. Studies were continuously being conducted to understand and develop efficient enzymes for the breakdown and ...
Ayyappa Kumar Sista Kameshwar, Wensheng Qin   +1 more
doaj   +1 more source